Bovine C-reactive protein,CRP ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E08577b）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CRP含量。CRP即C反應(yīng)蛋白，是一種急性時相反應(yīng)蛋白，在機(jī)體受到感染或組織損傷時，血漿中CRP水平會急劇上升。其可激活補(bǔ)體和加強(qiáng)吞噬細(xì)胞功能，在炎癥反應(yīng)中發(fā)揮重要作用。目前圍繞它在疾病診斷、病情監(jiān)測及藥物靶點(diǎn)等方面的研究較多。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，適用于獸醫(yī)研究、畜牧業(yè)動物健康監(jiān)測及炎癥相關(guān)分子機(jī)制探索等科研領(lǐng)域，例如通過動態(tài)監(jiān)測CRP水平評估牛群感染性疾病的發(fā)展趨勢或疫苗免疫效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CRP
Uniprot No.：

C4T8B4
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

1.25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-102
1:400	Average %	85
	Range %	81-88
1:800	Average %	89
	Range %	83-96

回收率：

The recovery of bovine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	97	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.