Bovine C-reactive protein,CRP ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛C反應蛋白(CRP)酶聯免疫試劑盒（CSB-E08577b）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CRP含量。CRP即C反應蛋白，是一種急性時相反應蛋白，在機體受到感染或組織損傷時，血漿中CRP水平會急劇上升。其可激活補體和加強吞噬細胞功能，在炎癥反應中發揮重要作用。目前圍繞它在疾病診斷、病情監測及藥物靶點等方面的研究較多。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，適用于獸醫研究、畜牧業動物健康監測及炎癥相關分子機制探索等科研領域，例如通過動態監測CRP水平評估牛群感染性疾病的發展趨勢或疫苗免疫效果。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CRP
Uniprot No.：

C4T8B4
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

1.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-102
1:400	Average %	85
	Range %	81-88
1:800	Average %	89
	Range %	83-96

回收率：

The recovery of bovine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	97	92-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.