Bovine Alpha-2-macroglobulin(A2M) ELISA kit

Bovine Alpha-2-macroglobulin(A2M) ELISA kit

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中文名稱：

牛α2巨球蛋白(A2M)酶聯免疫試劑盒
貨號：

CSB-EL001004BO
規格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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牛α2巨球蛋白(A2M)酶聯免疫試劑盒（CSB-EL001004BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的A2M含量。A2M即α?-巨球蛋白，是血漿中一種多功能糖蛋白。它能抑制多種蛋白水解酶活性，參與細胞的生長、修復等過程。在研究機制上，主要關注其對蛋白酶的捕獲作用、與細胞表面受體的相互作用，以及在疾病發生發展中所扮演的角色。試劑盒檢測范圍為0.312 μg/mL- 20 μg/mL，適用于科研場景中牛類動物模型的炎癥機制研究、免疫調控分析或蛋白酶活性相關疾病模型的分子機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

A2MAlpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
縮寫：

A2M
Uniprot No.：

Q7SIH1
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL- 20 μg/mL
靈敏度：

0.078 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine A2M in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	90-103
1:200	Average %	90
	Range %	82-94
1:400	Average %	101
	Range %	95-106
1:800	Average %	108
	Range %	101-112

回收率：

The recovery of bovine A2M spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-91
EDTA plasma (n=4)	93	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
20	0.127	0.131	0.129
10	0.248	0.233	0.241
5	0.490	0.523	0.507
2.5	0.909	0.911	0.910
1.25	1.439	1.464	1.452
0.625	1.853	1.789	1.821
0.312	2.460	2.400	2.430
0	2.795	2.702	2.749

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia Dudek K, et al,Bmc Veterinary Research,2019

功能：

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
基因功能參考文獻：
1. Mutations in the promoter region and the 3'-untranslated region of the A2M gene are involved in the mastitis susceptibility in dairy cows. PMID: 25237709
2. We report here for the first time the direct isolation of Spp24 covalently bound to alpha2-macroglobulins as a core complex (Spp24-alpha2M) from bovine calf serum. PMID: 22949401
3. Exon 29 c.3535A>T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis. PMID: 22923050
4. These results suggest the possible involvement of cathepsin E in disruption of the structural and functional integrity of alpha 2-macroglobulin in the endolysosome system. PMID: 12631277
5. Alpha2-macroglobulin is a substrate and an endogenous inhibitor for ADAMTS-4 and ADAMTS-5 PMID: 14715656
6. Amounts of alpha(2)-M receptor in granulosa cells were strongly inversely associated with concentrations of estradiol in dominant and subordinate follicles. PMID: 15001551
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亞細胞定位：

Secreted.
蛋白家族：

Protease inhibitor I39 (alpha-2-macroglobulin) family
組織特異性：

Plasma.
數據庫鏈接：

KEGG: bta:513856

STRING: 9913.ENSBTAP00000006167

UniGene: Bt.60794

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.