Biotin ELISA Kit
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中文名稱(chēng)：生物素檢測(cè)試劑
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貨號(hào)：CSB-E16209
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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促銷(xiāo)：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：生物素檢測(cè)試劑（CSB-E16209）檢測(cè)血清、血漿樣本中的Biotin含量。Biotin即生物素，它是水溶性維生素B族成員。作為羧化酶輔酶參與體內(nèi)多種重要代謝反應(yīng)。研究上，其常作為標(biāo)記物用于分子生物學(xué)和生物醫(yī)學(xué)，如蛋白質(zhì)純化、免疫檢測(cè)等，能與抗生物素蛋白特異結(jié)合，助力相關(guān)機(jī)制研究與應(yīng)用。試劑盒檢測(cè)范圍為12.5 pg/mL-200 pg/mL，靈敏度為4.986 pg/mL。適用于科研領(lǐng)域，包括但不限于膳食營(yíng)養(yǎng)研究、代謝性疾病動(dòng)物模型分析以及生物素相關(guān)分子機(jī)制探索，幫助研究者精準(zhǔn)量化微量生物素水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：Biotin
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樣本類(lèi)型：serum, plasma from human, mouse and other species
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檢測(cè)范圍：12.5 pg/mL-200 pg/mL
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靈敏度：4.986 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of Biotin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Determination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits E Kürk?ü Kahraman, O Donma,Journal of Clinical Laboratory Analysis,2025
- Prospective Analytical Controlled Study Evaluating Serum Biotin, Vitamin B12, and Folic Acid in Patients with Premature Canities Daulatabad D.et al,Int J Trichology,2017
產(chǎn)品評(píng)價(jià)
樣品類(lèi)型：蛋白（重組/純化） 蛋白 純化
樣品信息：人
稀釋比：1:100
產(chǎn)品評(píng)價(jià)： 我用biotin Elisa kit主要用于檢測(cè)純化后帶biotin的蛋白，經(jīng)實(shí)驗(yàn)檢測(cè)后發(fā)現(xiàn)標(biāo)曲符合要求，R2 大于0.98，經(jīng)標(biāo)曲換算后的蛋白濃度符合預(yù)期，試劑盒使用說(shuō)明準(zhǔn)確清晰。
By 李老師
相關(guān)問(wèn)答
Would you pls help to provide the following information regarding Biotin ELISA Kit(CSB-E16209):
1. What is the standard?
2. What is the antibody in this kit?
The standard is purified biotin；
This is a competitive kit and there is only mouse monoclonal antibody in the kit.
Pls let me know if you have any further questions. Thank you.
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