TPT1

TPT1蛋白，全稱為翻譯控制腫瘤蛋白（Translationally Controlled Tumor Protein），是一種高度保守的多功能蛋白，在細(xì)胞生長和增殖中發(fā)揮重要作用。它參與鈣結(jié)合和微管穩(wěn)定，作為負(fù)調(diào)節(jié)因子，可抑制細(xì)胞凋亡。TPT1蛋白常見別名包括TCTP，在多種生物過程中發(fā)揮關(guān)鍵作用。研究表明，TPT1蛋白與多種疾病相關(guān)，如肌萎縮側(cè)索硬化癥，其表達(dá)水平在患者血清中顯著升高，可作為潛在生物標(biāo)志物用于疾病診斷和監(jiān)測。此外，TPT1蛋白在腫瘤發(fā)生發(fā)展中也扮演重要角色，相關(guān)藥物研發(fā)正逐步深入，探索其作為治療靶點的可能性。

熱銷產(chǎn)品

Recombinant Human Translationally-controlled tumor protein (TPT1) (CSB-EP024134HU)

驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

TPT1 Recombinant Monoclonal Antibody (CSB-RA214107A0HU)

驗證數(shù)據(jù)

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
All lanes: TPT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa

IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating TCTP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA214107A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)

TPT1 Antibody

TPT1 Protein

TPT1 ELISA Kit

TPT1 Antibodies

TPT1 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA024134GA01HU	TPT1 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA588140	TPT1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA002968	TPT1 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA024134LA01HU	TPT1 Antibody	Human	ELISA, IHC, IF
CSB-PA024134LC01HU	TPT1 Antibody, FITC conjugated	Human
CSB-PA024134LD01HU	TPT1 Antibody, Biotin conjugated	Human	ELISA
CSB-RA214107A0HU	TPT1 Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, IHC, IF, IP

TPT1 Proteins

TPT1 Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP024134HU CSB-BP024134HU CSB-EP024134HU-B	Recombinant Human Translationally-controlled tumor protein (TPT1)	Yeast Baculovirus In Vivo Biotinylation in E.coli
CSB-EP024134HU	Recombinant Human Translationally-controlled tumor protein (TPT1)	E.coli
CSB-EP024134HUa0	Recombinant Human Translationally-controlled tumor protein (TPT1)	E.coli
CSB-MP024134HU	Recombinant Human Translationally-controlled tumor proteinv (SMPD1)	Mammalian cell