TFRC

TFRC，全稱轉鐵蛋白受體1，也被稱為CD71、TFR1、p90。該蛋白在細胞表面以同源二聚體的形式存在，對細胞生長至關重要。TFRC的主要功能是促進細胞鐵的吸收，通過受體介導的內吞作用將鐵離子從轉鐵蛋白中釋放到細胞內，從而維持細胞內鐵穩態。TFRC在多種腫瘤中高表達，并且與腫瘤的發生、侵襲和轉移有關，是一種重要的癌基因。其表達程度與疾病的惡性程度相關，過表達可促進腫瘤細胞生長、增殖和轉移，因此成為開發抗體偶聯藥物的新靶點。目前，全球已有多款針對TFRC的藥物進入臨床試驗階段，藥物類型涉及單抗、雙抗、融合蛋白、ADC等，其中Avidity的AOC-1001是全球首款AOC藥物，已進入臨床二期。此外，TFRC還參與了T細胞的激活過程，為T細胞的激活提供了所需的第二刺激信號，抗TFR1抗體有望成為研究淋巴細胞活化的一種新的高選擇性探針。

熱銷產品

Recombinant Human Transferrin receptor protein 1 (TFRC), partial (Active) (CSB-MP3648HU)

驗證數據

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2μg/mL can bind Anti-TFRC recombinant antibody (CSB-RA023441MA1HU)， the EC₅₀ is 3.305-8.220 ng/mL.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2 μg/ml can bind Human TF (CSB-MP023412HU). The EC₅₀ is 33.20-40.09 ng/mL.

TFRC Recombinant Monoclonal Antibody (CSB-RA023441MA1HU) captured on Protein A Chip can bind Recombinant Human TFRC protein with an affinity constant of 0.117 nM as detected by MetaSPR Assay (WeSPRTM 200).

TFRC Antibody (CSB-PA07219A0Rb)

驗證數據

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, MCF7 whole cell lysate
All lanes: TFRC antibody at 1:5000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa

IHC image of CSB-PA07219A0Rb diluted at 1:150 and staining in paraffin-embedded Human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-PA07219A0Rb diluted at 1:150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 cell with CSB-PA07219A0Rb at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Human soluble transferrin receptor,sTfR ELISA Kit (CSB-E09100h)

驗證數據

貨號：CSB-E09100h

規格：96T/48T

靈敏度：Request Information

檢測范圍：Request Information

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

TFRC Antibody

TFRC Protein

TFRC ELISA Kit

TFRC Antibodies

TFRC for Homo sapiens (Human)

產品貨號	產品名稱	種屬反應性	應用類型
CSB-PA023441GA01HU	TFRC Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA07219B0Rb	TFRC Antibody, HRP conjugated	Human	ELISA
CSB-PA07219D0Rb	TFRC Antibody, Biotin conjugated	Human	ELISA
CSB-PA07219A0Rb	TFRC Antibody	Human	ELISA, WB, IHC, IF
CSB-PA001477	TFRC Antibody	Human,Mouse	WB, IHC, ELISA
CSB-PA005607	TFRC Antibody	Human	WB, ELISA
CSB-PA010092	Phospho-TFRC (S24) Antibody	Human,Mouse	WB, IHC, ELISA
CSB-PA104885	TFRC Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA834425	TFRC Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-RA023441MA1HU	TFRC Recombinant Monoclonal Antibody	Human	ELISA

TFRC Proteins

TFRC Proteins for Equus caballus (Horse)

產品貨號	產品名稱	來源
CSB-YP651210HO CSB-EP651210HO CSB-BP651210HO CSB-MP651210HO CSB-EP651210HO-B	Recombinant Horse Transferrin receptor protein 1 (TFRC), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Pongo abelii (Sumatran orangutan)

產品貨號	產品名稱	來源
CSB-YP719275PYX CSB-EP719275PYX CSB-BP719275PYX CSB-MP719275PYX CSB-EP719275PYX-B	Recombinant Pongo abelii Transferrin receptor protein 1 (TFRC), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Mus musculus (Mouse)

產品貨號	產品名稱	來源
CSB-YP726792MO CSB-BP726792MO CSB-MP726792MO CSB-EP726792MO-B	Recombinant Mouse Transferrin receptor protein 1 (Tfrc), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Sus scrofa (Pig)

產品貨號	產品名稱	來源
CSB-YP816838PI CSB-BP816838PI CSB-MP816838PI CSB-EP816838PI-B	Recombinant Pig Transferrin receptor protein 1 (TFRC), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Gallus gallus (Chicken)

產品貨號	產品名稱	來源
CSB-YP856194CH CSB-EP856194CH CSB-BP856194CH CSB-MP856194CH CSB-EP856194CH-B	Recombinant Chicken Transferrin receptor protein 1 (TFRC), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Rattus norvegicus (Rat)

產品貨號	產品名稱	來源
CSB-YP858708RA1 CSB-BP858708RA1 CSB-MP858708RA1 CSB-EP858708RA1-B	Recombinant Rat Transferrin receptor protein 1 (Tfrc), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

產品貨號	產品名稱	來源
CSB-YP867082DO CSB-BP867082DO CSB-MP867082DO CSB-EP867082DO-B	Recombinant Dog Transferrin receptor protein 1 (TFRC), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TFRC Proteins for Felis catus (Cat) (Felis silvestris catus)

產品貨號	產品名稱	來源
CSB-YP882000CA CSB-EP882000CA CSB-BP882000CA CSB-MP882000CA CSB-EP882000CA-B	Recombinant Cat Transferrin receptor protein 1 (TFRC), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli