SMURF1
SMURF1，即Smad特異性E3泛素蛋白連接酶1，也稱(chēng)為hSMURF1、KIAA1625。SMURF1是一種HECT型E3泛素連接酶，主要在人體細(xì)胞中表達(dá)，尤其是在胚胎發(fā)育過(guò)程中的外胚層組織中高度表達(dá)。它的作用機(jī)制是通過(guò)與BMP（骨形態(tài)發(fā)生蛋白）通路中的Smad效應(yīng)子相互作用，導(dǎo)致Smad蛋白泛素化和降解，從而在調(diào)節(jié)細(xì)胞運(yùn)動(dòng)、細(xì)胞信號(hào)傳導(dǎo)和細(xì)胞極性方面起關(guān)鍵作用。SMURF1在細(xì)胞分化、組織形成和腫瘤發(fā)生等過(guò)程中具有重要的意義。SMURF1在體內(nèi)負(fù)調(diào)控成骨細(xì)胞分化和骨形成，并且與多種疾病相關(guān)，包括神經(jīng)管閉合缺陷、Brachydactyly Type A2、Fanconi貧血等。
熱銷(xiāo)產(chǎn)品
Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial (CSB-EP875720HU1a0)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
SMURF1 Antibody (CSB-PA875720LA01HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: U251 whole cell lysate, A549 whole cell lysate
All lanes: SMURF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 84 KDa
Observed band size: 87 KDa
Exposure time：5min
Immunofluorescence staining of A549 cells with CSB-PA875720LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
SMURF1 Antibodies
SMURF1 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-PA021860GA01HU | SMURF1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA026284 | SMURF1 Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA875720LA01HU | SMURF1 Antibody | Human | ELISA, WB, IF |
| CSB-PA875720LB01HU | SMURF1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA875720LC01HU | SMURF1 Antibody, FITC conjugated | Human | |
| CSB-PA875720LD01HU | SMURF1 Antibody, Biotin conjugated | Human | ELISA |
| CSB-MA875720A0m | SMURF1 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
SMURF1 Proteins
SMURF1 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP875137MO CSB-EP875137MO CSB-BP875137MO CSB-MP875137MO CSB-EP875137MO-B |
Recombinant Mouse E3 ubiquitin-protein ligase SMURF1 (Smurf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SMURF1 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP875720HU CSB-EP875720HU CSB-BP875720HU CSB-MP875720HU CSB-EP875720HU-B |
Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP875720HU1 | Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial | E.coli |
| CSB-EP875720HU1a0 | Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial | E.coli |
SMURF1 Proteins for Xenopus laevis (African clawed frog)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP886105XBE CSB-EP886105XBE CSB-BP886105XBE CSB-MP886105XBE CSB-EP886105XBE-B |
Recombinant Xenopus laevis E3 ubiquitin-protein ligase SMURF1 (smurf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SMURF1 ELISA Kit
SMURF1 ELISA Kit for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 樣本類(lèi)型 | 靈敏度 |
|---|---|---|---|
| CSB-EL021860MO | Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 7.8 pg/mL |
