SKP2
SKP2，即S期激酶相關(guān)蛋白2，別名p45、FBXW2、SKP2。SKP2是E3泛素連接酶SCF復(fù)合體的組成元件之一，包含F(xiàn)-box序列、連接子序列、C-末端及10個(gè)富含亮氨酸重復(fù)序列結(jié)構(gòu)域（LRR），其中LRR在底物識別中起重要作用。SKP2可誘導(dǎo)K48連接的泛素化，導(dǎo)致蛋白酶體介導(dǎo)的標(biāo)記底物的蛋白水解，影響多種細(xì)胞周期相關(guān)蛋白的穩(wěn)定性，如p27、FOXO1和CARM1等。SKP2通過控制細(xì)胞周期相關(guān)蛋白的穩(wěn)定性，參與細(xì)胞周期調(diào)控、增殖、遷移和腫瘤發(fā)生發(fā)展，其表達(dá)水平與多種腫瘤的發(fā)生、發(fā)展、預(yù)后密切相關(guān)。
熱銷產(chǎn)品
Recombinant Human S-phase kinase-associated protein 2 (SKP2) (CSB-EP613392HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
SKP2 Recombinant Monoclonal Antibody (CSB-RA155372A0HU)
驗(yàn)證數(shù)據(jù)
Immunofluorescence staining of HepG2 Cells with CSB-RA155372A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA155372A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
SKP2 Antibodies
SKP2 for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA021362GA01HU | SKP2 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA613392LA01HU | SKP2 Antibody | Human | ELISA, WB, IHC |
| CSB-PA613392LD01HU | SKP2 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA004098 | SKP2 Antibody | Human | WB, IHC, IF, ELISA |
| CSB-RA155372A0HU | SKP2 Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
| CSB-RA224072A0HU | SKP2 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF |
SKP2 Proteins
SKP2 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP331655SVG CSB-EP331655SVG CSB-BP331655SVG CSB-MP331655SVG CSB-EP331655SVG-B |
Recombinant Saccharomyces cerevisiae F-box protein SKP2 (SKP2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SKP2 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP613392HU CSB-BP613392HU CSB-MP613392HU CSB-EP613392HU-B |
Recombinant Human S-phase kinase-associated protein 2 (SKP2) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP613392HU | Recombinant Human S-phase kinase-associated protein 2 (SKP2) | E.coli |
