RAF1
RAF1，即絲裂原活化蛋白激酶激酶1，別名CRAF、Raf-1、Raf激酶等。它是一種由RAF1基因編碼的蛋白激酶，屬于RAF家族成員，在多種細(xì)胞類型中表達(dá)。RAF1的作用機(jī)制涉及作為絲裂原活化蛋白激酶（MAPK）信號(hào)通路中的一個(gè)關(guān)鍵調(diào)節(jié)因子，通過磷酸化并激活下游的MEK1/2，進(jìn)而激活ERK1/2，調(diào)控細(xì)胞增殖、分化、遷移和存活。RAF1參與了多種細(xì)胞生命活動(dòng)的調(diào)控，包括細(xì)胞周期控制、細(xì)胞應(yīng)激反應(yīng)、細(xì)胞凋亡等。RAF1的功能異常與多種疾病有關(guān)，尤其是與癌癥的發(fā)生發(fā)展密切相關(guān)。RAF1的突變或異常激活可能導(dǎo)致腫瘤細(xì)胞的無限制增殖和抗藥性，使其成為癌癥治療中的一個(gè)潛在靶點(diǎn)。
熱銷產(chǎn)品
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) (CSB-EP019284HUc7)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Phospho-RAF1 (S621) Recombinant Monoclonal Antibody (CSB-RA019284A621phHU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.525μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA019284A621phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
RAF1 Antibodies
RAF1 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA019284LA01HU | RAF1 Antibody | Human | ELISA, WB, IHC, IF, IP |
| CSB-PA291059 | Phospho-RAF1 (Ser259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA151308 | Phospho-RAF1 (Ser338) Antibody | Human,Mouse | ELISA,WB |
| CSB-PA242762 | RAF1 (Ab-259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA209480 | RAF1 (Ab-338) Antibody | Human,Mouse | ELISA,WB,IHC |
| CSB-PA445340 | Phospho-RAF1 (Ser621) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA580751 | Phospho-RAF1 (Tyr341) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA299750 | Phospho-RAF1 (Thr269) Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA148049 | Phospho-RAF1 (Ser642) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA948259 | Phospho-RAF1 (Ser301) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA000615 | Phospho-RAF1 (S259) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
| CSB-PA000660 | Phospho-RAF1 (S338) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA003921 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
| CSB-PA003922 | RAF1 Antibody | Human,Mouse,Rat,Monkey | WB, IF, ELISA |
| CSB-PA003923 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA003926 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA040148 | Phospho-RAF1 (S43) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA040149 | Phospho-RAF1 (S301) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA040150 | Phospho-RAF1 (S642) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA040151 | Phospho-RAF1 (Y341) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA040152 | Phospho-RAF1 (S296) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA040153 | Phospho-RAF1 (S621) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
| CSB-PA040154 | Phospho-RAF1 (T269) Antibody | Human,Mouse,Rat | IHC, ELISA |
| CSB-PA040155 | Phospho-RAF1 (S289) Antibody | Human,Mouse,Rat | IHC, ELISA |
| CSB-PA040156 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA040157 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA040158 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA040160 | RAF1 Antibody | Human,Mouse,Rat | IHC, ELISA |
| CSB-PA080220 | RAF1 Antibody | Human | WB, IHC |
| CSB-PA010547 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA694669 | RAF1 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-RA019284A621phHU | Phospho-RAF1 (S621) Recombinant Monoclonal Antibody | Human | ELISA, WB, IF |
| CSB-RA019284A259phHU | Phospho-RAF1 (S259) Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF |
| CSB-RA019284A43phHU | Phospho-RAF1 (S43) Recombinant Monoclonal Antibody | Human | ELISA, IF |
RAF1 for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA950764 | Raf1 Antibody | Rat | ELISA,WB |
RAF1 Proteins
RAF1 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP019284RA CSB-EP019284RA CSB-BP019284RA CSB-MP019284RA CSB-EP019284RA-B |
Recombinant Rat RAF proto-oncogene serine/threonine-protein kinase (Raf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP019284HU CSB-BP019284HU CSB-MP019284HU CSB-EP019284HU-B |
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP019284HU | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | E.coli |
| CSB-EP019284HUc7 | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | E.coli |
RAF1 Proteins for Gallus gallus (Chicken)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP019284CH CSB-EP019284CH CSB-BP019284CH CSB-MP019284CH CSB-EP019284CH-B |
Recombinant Chicken RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP019284BO CSB-EP019284BO CSB-BP019284BO CSB-MP019284BO CSB-EP019284BO-B |
Recombinant Bovine RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Xenopus laevis (African clawed frog)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP019284XBE CSB-EP019284XBE CSB-BP019284XBE CSB-MP019284XBE CSB-EP019284XBE-B |
Recombinant Xenopus laevis RAF proto-oncogene serine/threonine-protein kinase (raf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP524907SXV CSB-EP524907SXV CSB-BP524907SXV CSB-MP524907SXV CSB-EP524907SXV-B |
Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Pongo abelii (Sumatran orangutan)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP722278PYX CSB-EP722278PYX CSB-BP722278PYX CSB-MP722278PYX CSB-EP722278PYX-B |
Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP859572MO CSB-EP859572MO CSB-BP859572MO CSB-MP859572MO CSB-EP859572MO-B |
Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
