PDCD1

PDCD1，即程序性細(xì)胞死亡蛋白1，也稱為PD1、CD279、hSLE1，由人類PDCD1基因編碼，該基因位于2號染色體的q37.3位置。PDCD1蛋白是一種重要的免疫抑制受體，屬于免疫球蛋白超家族成員，主要在激活的T細(xì)胞和B細(xì)胞上表達(dá)
其作用機(jī)制涉及與配體PD-L1和PD-L2的結(jié)合，啟動(dòng)T細(xì)胞的程序性死亡，從而抑制T細(xì)胞的活化和增殖。
PDCD1在維持外周T、B細(xì)胞耐受中發(fā)揮重要作用，其增強(qiáng)信號可以下調(diào)已活化的T、B細(xì)胞在外周組織中的應(yīng)答。PDCD1的異常表達(dá)與多種自身免疫疾病、感染性疾病和腫瘤相關(guān)，是免疫治療的重要靶點(diǎn)。

熱銷產(chǎn)品

Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active) (CSB-MP619964HU1)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
①M(fèi)easured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC₅₀ of human PD-1 protein is 6.087-7.854 ng/ml.

Activity
②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC₅₀ of human PD-1 protein is 9.713-12.39 ng/ml.

The purity of PDCD1 was greater than 95% as determined by SEC-HPLC

PDCD1 Recombinant Monoclonal Antibody (CSB-RA240597A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: PD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 kDa

IHC image of CSB-RA240597A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

PDCD1 Antibody

PDCD1 Protein

PDCD1 ELISA Kit

PDCD1 Antibodies

PDCD1 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA017664GA01HU	PDCD1 Antibody	Human	ELISA,WB,IHC
CSB-PA483633	PDCD1 Antibody	Human	ELISA,IHC
CSB-PA619964LA01HU	PDCD1 Antibody	Human, Mouse	ELISA, WB, IHC, IF
CSB-PA619964LD01HU	PDCD1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA619964LC01HU	PDCD1 Antibody, FITC conjugated	Human
CSB-PA619964LB01HU	PDCD1 Antibody, HRP conjugated	Human	ELISA
CSB-RA240597A0HU	PDCD1 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC
CSB-MA619964A0m	PD-1 Monoclonal Antibody	Human	ELISA, WB
CSB-MA619964A1m	PD-1 Monoclonal Antibody	Human	ELISA, WB
CSB-RA619964MA1HU	PD-1 Recombinant Monoclonal Antibody	Human	ELISA

PDCD1 Proteins

PDCD1 Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP619964HU CSB-BP619964HU CSB-MP619964HU CSB-EP619964HU-B	Recombinant Human Programmed cell death protein 1 (PDCD1), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP619964HU	Recombinant Human Programmed cell death protein 1 (PDCD1), partial	E.coli
CSB-MP619964HU1	Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active)	Mammalian cell