NPHS1

NPHS1，別稱CNF、NPHN，是腎小球濾過屏障結構中的關鍵蛋白，位于足細胞裂孔隔膜。該蛋白在維持腎小球濾過功能中起著至關重要的作用，其缺陷與芬蘭型先天性腎病綜合癥相關，導致蛋白質大量泄漏至尿液中，引發蛋白尿。NPHS1蛋白的表達和功能異常，不僅影響腎小球的正常過濾功能，還可能對心血管系統的發展造成影響。目前，針對NPHS1相關疾病的藥物研發主要集中在抗-Nephrin抗體，這類抗體在動物模型中顯示出誘導蛋白尿和腎病綜合征的潛力。然而，在人體內的應用仍需謹慎評估，因為抗-Nephrin抗體可能成為腎移植后復發的發病機制之一。此外，對Nephrin蛋白不同表位和抗體的相互作用機制研究，以及開發針對抗-Nephrin抗體陽性患者的特異性藥物，是未來研究的重點方向。

熱銷產品

Recombinant Human Nephrin (NPHS1), partial (CSB-EP015988HU)

驗證數據

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

NPHS1 Antibody (CSB-PA015988LA01HU)

驗證數據

WPositive WB detected in: JK whole cell lysate30μg), U251 whole cell lysate(30μg), MCF7 whole cell lysate(30μg), 293T whole cell lysate(30μg)
All lanes: NPHS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135,131 kDa
Observed band size: 135 kDa
Exposure time：120s

IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Human Nephrin ELISA Kit (CSB-E09886h)

驗證數據

貨號：CSB-E09886h

規格：96T/48T

靈敏度：0.39 ng/ml

檢測范圍：1.56 ng/ml-100ng/ml

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

NPHS1 Antibody

NPHS1 Protein

NPHS1 ELISA Kit

NPHS1 Antibodies

NPHS1 for Homo sapiens (Human)

產品貨號	產品名稱	種屬反應性	應用類型
CSB-PA015988LA01HU	NPHS1 Antibody	Human	ELISA, WB, IHC
CSB-PA015988LB01HU	NPHS1 Antibody, HRP conjugated	Human	ELISA
CSB-PA015988LD01HU	NPHS1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA020148	NPHS1 Antibody	Human,Mouse,Rat	IHC, ELISA
CSB-PA909441	NPHS1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA967591	NPHS1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC