G3BP1
G3BP1，全稱Ras-GTPase-activating protein SH3 domain-binding protein 1，別名G3BP、HDH-VIII，是一種在細胞應激反應中發揮重要作用的蛋白質。它參與應激顆粒的組裝，并能夠調節細胞的鐵死亡過程。G3BP1在多種惡性腫瘤組織中表達上調，例如乳腺癌、結腸癌、頭頸部癌等，其高表達與腫瘤細胞的增殖、侵襲和轉移密切相關。此外，G3BP1還在宮頸癌、膀胱癌等腫瘤的發生發展中發揮重要作用，并可能成為腫瘤治療的潛在靶點。目前，針對G3BP1的藥物研發正在積極開展，例如設計合成靶向G3BP1的PROTAC分子，以降解G3BP1蛋白，從而抑制腫瘤細胞的生長和轉移。此外，G3BP1也被發現與溶酶體自噬活性相關，參與調節細胞的鐵死亡過程，為治療椎間盤退變等疾病提供了新的思路。
熱銷產品
Recombinant Human Ras GTPase-activating protein-binding protein 1 (G3BP1) (CSB-EP613389HU(A4)a0)
驗證數據
a0-SDS.jpg)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
G3BP1 Recombinant Monoclonal Antibody (CSB-RA583845A0HU)
驗證數據
Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), JURKAT whole cell lysate(30μg), K562 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: G3BP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time：1min
IHC image of CSB-RA583845A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA583845A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA583845A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
G3BP1 Antibodies
G3BP1 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA009116GA01HU | G3BP1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA613389ESR1HU | G3BP1 Antibody | Human | ELISA, WB, IHC |
| CSB-PA908450 | Phospho-G3BP1 (Ser232) Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA057449 | G3BP1 (Ab-232) Antibody | Human | ELISA,WB,IHC |
| CSB-PA000531 | Phospho-G3BP1 (S232) Antibody | Human,Mouse | WB, IHC, ELISA |
| CSB-PA002599 | G3BP1 Antibody | Human,Mouse,Monkey | WB, IHC, ELISA |
| CSB-RA583845A0HU | G3BP1 Recombinant Monoclonal Antibody | Human, Mouse | ELISA, IF, FC |
G3BP1 Proteins
G3BP1 Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009116MO CSB-EP009116MO CSB-BP009116MO CSB-MP009116MO CSB-EP009116MO-B |
Recombinant Mouse Ras GTPase-activating protein-binding protein 1 (G3bp1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
G3BP1 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP613389HU CSB-EP613389HU CSB-BP613389HU CSB-MP613389HU CSB-EP613389HU-B |
Recombinant Human Ras GTPase-activating protein-binding protein 1 (G3BP1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP613389HU(A4)a0 | Recombinant Human Ras GTPase-activating protein-binding protein 1 (G3BP1) | E.coli |
G3BP1 Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP654171BO CSB-EP654171BO CSB-BP654171BO CSB-MP654171BO CSB-EP654171BO-B |
Recombinant Bovine Ras GTPase-activating protein-binding protein 1 (G3BP) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
G3BP1 Proteins for Pongo abelii (Sumatran orangutan)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP712947PYX CSB-EP712947PYX CSB-BP712947PYX CSB-MP712947PYX CSB-EP712947PYX-B |
Recombinant Pongo abelii Ras GTPase-activating protein-binding protein 1 (G3BP1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
