CEACAM5
CEACAM5，即癌胚抗原細(xì)胞黏附分子5，別名CD66e，由人類(lèi)CEACAM5基因編碼，該基因位于染色體19q13.2，基因長(zhǎng)度約為21kb，由9個(gè)外顯子和3個(gè)非編碼外顯子組成。CEACAM5通過(guò)糖基磷脂酰肌醇（GPI）與膜結(jié)合，胞外共有7個(gè)免疫球蛋白樣結(jié)構(gòu)域，包括一個(gè)可變（IgV）樣結(jié)構(gòu)域和6個(gè)IgC樣結(jié)構(gòu)域（A1-B3）。
CEACAM5在多種生物學(xué)過(guò)程中發(fā)揮作用，包括細(xì)胞黏附、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和免疫調(diào)節(jié)等，并在許多惡性腫瘤細(xì)胞中高表達(dá)，被廣泛用于癌癥的診斷和監(jiān)測(cè)。CEACAM5的表達(dá)異常與多種癌癥的發(fā)生發(fā)展相關(guān)，尤其在結(jié)直腸癌、胃癌、肺癌和胰腺癌中過(guò)表達(dá)，是潛在的癌癥治療靶點(diǎn)。
熱銷(xiāo)產(chǎn)品
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA1HU), the EC50 is 0.8955-1.719 ng/mL.
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CEACAM5 at 2μg/ml can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA4HU). The EC50 is 3.572-4.044 ng/mL.
The purity of CEACAM5 was greater than 95% as determined by SEC-HPLC
CEACAM5 Recombinant Monoclonal Antibody (CSB-RA005165MA3HU)
驗(yàn)證數(shù)據(jù)
Immunofluorescence staining of Hela cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).
Immunofluorescence staining of MCF7 cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).
Overlay Peak curve showing Hela cells surface stained with CSB-RA005165MA3HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was human IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.
CEACAM5 Antibodies
CEACAM5 for H
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-MA000218 | CEACAM5 Monoclonal Antibody | Human | ELISA,IHC |
CEACAM5 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-PA005033 | CEACAM5 Antibody | Human | WB, ELISA |
| CSB-PA160116 | CEACAM5 Antibody | Human | ELISA,WB,IHC |
| CSB-PA229032 | CEACAM5 Antibody | Human | ELISA,WB,IHC |
| CSB-PA109108 | CEACAM5 Antibody | Human | ELISA,IHC |
| CSB-PA097333 | CEACAM5 Antibody | Human | ELISA,IHC |
| CSB-PA005165LA01HU | CEACAM5 Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA005165LB01HU | CEACAM5 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA005165LC01HU | CEACAM5 Antibody, FITC conjugated | Human | |
| CSB-PA005165LD01HU | CEACAM5 Antibody, Biotin conjugated | Human | ELISA |
| CSB-MA995583 | CEACAM5 Monoclonal Antibody | Human | ELISA, IHC |
| CSB-RA005165MA3HU | CEACAM5 Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
| CSB-RA005165MA1HU | CEACAM5 (labetuzumab) Recombinant Monoclonal Antibody | Human | ELISA |
CEACAM5 Proteins
CEACAM5 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005165HU | Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K) | Yeast |
| CSB-MP005165HU | Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K) (Active) | Mammalian cell |
CEACAM5 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005165MO CSB-EP005165MO CSB-BP005165MO CSB-EP005165MO-B |
Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CEACAM5 ELISA Kit
CEACAM5 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 樣本類(lèi)型 | 靈敏度 |
|---|---|---|---|
| CSB-E04767h | Human carcinoembryonic antigen,CEA ELISA Kit | serum, plasma, tissue homogenates | 2 ng/mL |
CEACAM5 ELISA Kit for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 樣本類(lèi)型 | 靈敏度 |
|---|---|---|---|
| CSB-E13925m | Mouse carcinoembryonic antign,CEA ELISA Kit | serum, plasma, tissue homogenates | 15.6 pg/mL |
