CDK4
CDK4，即細(xì)胞周期蛋白依賴(lài)性激酶4，別名P15INK4B結(jié)合伴侶、P16INK4A結(jié)合伴侶、CDKN2A/p16INK4a互作伴侶等。它是一種由CDK4基因編碼的絲氨酸/蘇氨酸激酶，屬于細(xì)胞周期調(diào)控蛋白家族成員。CDK4在細(xì)胞周期的G1期向S期轉(zhuǎn)換中發(fā)揮關(guān)鍵作用，主要通過(guò)與周期蛋白D（cyclin D）形成復(fù)合體，磷酸化視網(wǎng)膜母細(xì)胞瘤蛋白（Rb），從而促進(jìn)Rb蛋白的去磷酸化，釋放E2F家族轉(zhuǎn)錄因子，推動(dòng)細(xì)胞周期進(jìn)程。CDK4在生物學(xué)上的意義非常廣泛，它參與調(diào)控細(xì)胞周期、細(xì)胞增殖、分化和衰老等重要生物學(xué)過(guò)程。CDK4的活性受到多種因素的精細(xì)調(diào)控，包括周期蛋白的合成、降解以及CDK抑制因子（CKIs）的抑制作用。CDK4的功能異常與多種疾病有關(guān)，尤其是與腫瘤的發(fā)生發(fā)展密切相關(guān)。例如，在多種腫瘤中觀察到CDK4的過(guò)表達(dá)或Rb蛋白功能喪失，導(dǎo)致細(xì)胞周期失控和腫瘤細(xì)胞的無(wú)限制增殖。因此，CDK4是腫瘤治療中的一個(gè)潛在靶點(diǎn)，針對(duì)CDK4的小分子抑制劑正在被開(kāi)發(fā)用于治療某些類(lèi)型的癌癥。
熱銷(xiāo)產(chǎn)品
Recombinant Human Cyclin-dependent kinase 4 (CDK4) (CSB-EP005065HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CDK4 Recombinant Monoclonal Antibody (CSB-RA965615A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: CDK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa
Immunoprecipitating CDK4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA965615A0HU in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)
Lane 2:CSB-RA965615A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)
CDK4 Antibodies
CDK4 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-PA005065GA01HU | CDK4 Antibody | Human,Mouse,Rat | ELISA,WB,IF |
| CSB-PA11659D0Rb | CDK4 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA11659B0Rb | CDK4 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA11659C0Rb | CDK4 Antibody, FITC conjugated | Human | |
| CSB-PA11659A0Rb | CDK4 Antibody | Human | ELISA, IHC |
| CSB-PA275368 | CDK4 Antibody | Human | ELISA,WB,IHC |
| CSB-PA005118 | CDK4 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA597701 | CDK4 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-RA965615A0HU | CDK4 Recombinant Monoclonal Antibody | Human | ELISA, WB, IP |
| CSB-RA697694A0HU | CDK4 Recombinant Monoclonal Antibody | Human | ELISA, WB |
CDK4 Proteins
CDK4 Proteins for Sus scrofa (Pig)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065PI CSB-EP005065PI CSB-BP005065PI CSB-MP005065PI CSB-EP005065PI-B |
Recombinant Pig Cyclin-dependent kinase 4 (CDK4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDK4 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065HU CSB-MP005065HU CSB-EP005065HU-B |
Recombinant Human Cyclin-dependent kinase 4 (CDK4) | Yeast Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP005065HU | Recombinant Human Cyclin-dependent kinase 4 (CDK4) | E.coli |
CDK4 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065MO CSB-EP005065MO CSB-BP005065MO CSB-MP005065MO CSB-EP005065MO-B |
Recombinant Mouse Cyclin-dependent kinase 4 (Cdk4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDK4 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065RA CSB-EP005065RA CSB-BP005065RA CSB-MP005065RA CSB-EP005065RA-B |
Recombinant Rat Cyclin-dependent kinase 4 (Cdk4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDK4 Proteins for Xenopus laevis (African clawed frog)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065XBE CSB-EP005065XBE CSB-BP005065XBE CSB-MP005065XBE CSB-EP005065XBE-B |
Recombinant Xenopus laevis Cyclin-dependent kinase 4 (cdk4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDK4 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP005065BO CSB-EP005065BO CSB-BP005065BO CSB-MP005065BO CSB-EP005065BO-B |
Recombinant Bovine Cyclin-dependent kinase 4 (CDK4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDK4 Proteins for Homo sapiens(Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-BP005065HU | Recombinant Human Cyclin-dependent kinase4 (CDK4) | Baculovirus |
CDK4 ELISA Kit
CDK4 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 樣本類(lèi)型 | 靈敏度 |
|---|---|---|---|
| CSB-E09174h | Human Cyclin-dependent kinase 4,CDK-4 ELISA Kit | serum, plasma | 0.039 ng/mL |
