CD274

CD274，即細胞程序性死亡-配體1，別名PD-L1、PDCD1 ligand 1、hPD-L1。CD274編碼的蛋白質(zhì)是一種分子量為40kDa的I型跨膜蛋白，屬于CD分子和B7家族成員。其作用機制涉及與PD-1受體結(jié)合后，傳導抑制性的信號，減低CD8+T細胞的增生，并可能通過調(diào)節(jié)Bcl-2基因控制淋巴結(jié)中抗原特異性T細胞的聚積。CD274在免疫系統(tǒng)的抑制過程中可能發(fā)揮著重要作用，例如在妊娠、組織移植、自體免疫疾病以及其他疾病如肝炎中。正常情況下，免疫系統(tǒng)對外來抗原產(chǎn)生反應(yīng)，引發(fā)抗原特異性CD8+T細胞的增殖，而PD-1與CD274結(jié)合傳遞抑制信號，從而抑制CD8+T細胞的增殖。CD274的表達在多種類型的人類惡性腫瘤中具有預(yù)后意義，并在腫瘤微環(huán)境中通過抑制細胞毒性T細胞的活性為腫瘤細胞提供免疫逃逸。

熱銷產(chǎn)品

Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial (Active) (CSB-MP878942HU1)

驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC₅₀ of human PD-L1 protein is 1.252-1.653 ng/mL.

The purity of CD274 was greater than 90% as determined by SEC-HPLC.

CD274 Recombinant Monoclonal Antibody (CSB-RA878942MA1HU)

驗證數(shù)據(jù)

IHC image of CSB-RA878942MA1HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing Hela cells surface stained with CSB-RA878942MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

CD274 Antibody

CD274 Protein

CD274 ELISA Kit

CD274 Antibodies

CD274 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA004911GA01HU	CD274 Antibody	Human,Mouse,Rat	WB, IP, IHC, IF, FC, ELISA
CSB-PA878942ESR1HU	CD274 Antibody	Human	ELISA, IHC
CSB-MA878942A0m	PD-L1 Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC
CSB-MA878942A1m	PD-L1 Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC
CSB-RA977797A0HU	CD274 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC
CSB-RA878942MA1HU	CD274 Recombinant Monoclonal Antibody	Human	ELISA, IHC, FC

CD274 Proteins

CD274 Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP878942HU1 CSB-EP878942HU1 CSB-BP878942HU1 CSB-EP878942HU1-B	Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-MP878942HU1	Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial (Active)	Mammalian cell
CSB-EP878942HU2	Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial	E.coli
CSB-EP878942HU1(M)	Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial ( (K136A))	E.coli
CSB-YP878942HU1(M)c7	Recombinant Human Programmed cell death 1 ligand 1 (CD274) (K136A), partial	Yeast

CD274 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP880615MO CSB-EP880615MO CSB-BP880615MO CSB-MP880615MO CSB-EP880615MO-B	Recombinant Mouse Programmed cell death 1 ligand 1 (Cd274), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli