CANX
鈣連接蛋白（Calnexin，別名IP90）是一種廣泛表達(dá)于內(nèi)質(zhì)網(wǎng)的分子伴侶蛋白，主要負(fù)責(zé)協(xié)助新生糖蛋白的正確折疊與質(zhì)量控制。CANX通過識別未完全折疊蛋白的糖基化修飾，與鈣網(wǎng)蛋白協(xié)同形成"折疊檢查系統(tǒng)"，阻止錯誤折疊蛋白分泌或促進(jìn)其進(jìn)入降解途徑。該蛋白在維持內(nèi)質(zhì)網(wǎng)穩(wěn)態(tài)中發(fā)揮關(guān)鍵作用，其功能異常與阿爾茨海默病、帕金森病等神經(jīng)退行性疾病密切相關(guān)，錯誤折疊蛋白的積累會觸發(fā)細(xì)胞凋亡通路。在腫瘤領(lǐng)域，某些癌細(xì)胞通過上調(diào)鈣連接蛋白增強(qiáng)應(yīng)激耐受性，促進(jìn)轉(zhuǎn)移和耐藥性形成。CANX還參與調(diào)控免疫細(xì)胞表面MHC-I類分子的組裝，影響抗原呈遞過程。針對其相關(guān)通路開發(fā)的分子伴侶抑制劑已進(jìn)入臨床前研究，如選擇性抑制內(nèi)質(zhì)網(wǎng)應(yīng)激通路的小分子化合物，在乳腺癌和骨髓瘤模型中顯示出增強(qiáng)化療敏感性的潛力。基于其多重生物學(xué)功能，鈣連接蛋白已成為神經(jīng)疾病和腫瘤治療的新興靶點。
熱銷產(chǎn)品
CANX Monoclonal Antibody (CSB-MA004485A1m)
驗證數(shù)據(jù)
Western Blot
Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
All lanes: CANX antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 68, 72, 56 kDa
Observed band size: 90 KDa
Exposure time：1min
Immunofluorescence staining of Hela cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of HepG2 cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay Peak curve showing Hela cells stained with CSB-MA004485A1m (red line) at 1:140. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
CANX Antibodies
CANX for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA004485GA01HU | CANX Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA000488 | Phospho-CANX (S583) Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
| CSB-PA001175 | CANX Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
| CSB-PA080219 | CANX Antibody | Human,Mouse,Rat | WB, IHC |
| CSB-PA106469 | CANX Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA798312 | CANX Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA00899A0Rb | CANX Antibody | Human | ELISA, WB, IHC, IF |
| CSB-MA004485A0m | CANX Monoclonal Antibody | Human | ELISA, WB, FC |
| CSB-MA004485A1m | CANX Monoclonal Antibody | Human | ELISA, WB, IF, FC |
CANX Proteins
CANX Proteins for Canis familiaris (Dog) (Canis lupus familiaris)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004485DO1 CSB-EP004485DO1 CSB-BP004485DO1 CSB-MP004485DO1 CSB-EP004485DO1-B |
Recombinant Dog Calnexin (CANX), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004485DO | Recombinant Dog Calnexin (CANX) | in vitro E.coli expression system |
CANX Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004485MO1 CSB-EP004485MO1 CSB-BP004485MO1 CSB-MP004485MO1 CSB-EP004485MO1-B |
Recombinant Mouse Calnexin (Canx), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004485MO | Recombinant Mouse Calnexin (Canx) | in vitro E.coli expression system |
CANX Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004485HU1 CSB-EP004485HU1 CSB-BP004485HU1 CSB-MP004485HU1 CSB-EP004485HU1-B |
Recombinant Human Calnexin (CANX), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004485HU | Recombinant Human Calnexin (CANX) | in vitro E.coli expression system |
CANX Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004485RA1 CSB-EP004485RA1 CSB-BP004485RA1 CSB-MP004485RA1 CSB-EP004485RA1-B |
Recombinant Rat Calnexin (Canx), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004485RA | Recombinant Rat Calnexin (Canx) | in vitro E.coli expression system |
CANX Proteins for Pongo abelii (Sumatran orangutan)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004485PYX CSB-EP004485PYX CSB-BP004485PYX CSB-MP004485PYX CSB-EP004485PYX-B |
Recombinant Pongo abelii Calnexin (CANX), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
