BRD4

BRD4，即溴結(jié)構(gòu)域蛋白4，別名BRD4。BRD4是一種屬于溴結(jié)構(gòu)域和超末端結(jié)構(gòu)域（BET）家族的蛋白，主要通過識別乙酰化組蛋白來調(diào)節(jié)基因表達(dá)。BRD4的作用機(jī)制主要涉及其與轉(zhuǎn)錄因子的相互作用。BRD4通過其兩個(gè)溴結(jié)構(gòu)域（BD1和BD2）結(jié)合乙酰化的組蛋白，定位于目標(biāo)基因的啟動(dòng)子或增強(qiáng)子區(qū)域，促進(jìn)相關(guān)基因的轉(zhuǎn)錄。此外，BRD4還通過招募轉(zhuǎn)錄延伸因子和其他調(diào)節(jié)因子，增強(qiáng)RNA聚合酶II的活性，從而促進(jìn)基因的轉(zhuǎn)錄伸長。BRD4在細(xì)胞周期、增殖和凋亡等生理過程中起著關(guān)鍵作用。研究表明，BRD4的異常表達(dá)與多種惡性腫瘤的發(fā)生發(fā)展相關(guān)，包括急性髓系白血病、多發(fā)性骨髓瘤、乳腺癌和膠質(zhì)母細(xì)胞瘤等。BRD4不僅參與腫瘤細(xì)胞的增殖和轉(zhuǎn)移，還在DNA損傷修復(fù)和端粒維持中發(fā)揮重要作用。因此，BRD4成為了癌癥治療的一個(gè)重要靶點(diǎn)。

熱銷產(chǎn)品

Recombinant Human Bromodomain-containing protein 4 (BRD4), partial (CSB-EP002802HU)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

BRD4 Recombinant Monoclonal Antibody (CSB-RA152430A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: BRD4 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 153, 81, 89 kDa
Observed band size: 153 kDa

IHC image of CSB-RA152430A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA152430A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

BRD4 Antibody

BRD4 Protein

BRD4 ELISA Kit

BRD4 Antibodies

BRD4 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA577233	BRD4 Antibody	Human,Mouse	ELISA,WB
CSB-PA249846	BRD4 Antibody	Human,Mouse	ELISA,WB
CSB-PA002802LA01HU	BRD4 Antibody	Human	ELISA, IF
CSB-PA002802LD01HU	BRD4 Antibody, Biotin conjugated	Human	ELISA
CSB-RA152430A0HU	BRD4 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC

BRD4 Proteins

BRD4 Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP002802HU CSB-BP002802HU CSB-MP002802HU CSB-EP002802HU-B	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP002802HU	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	E.coli
CSB-EP002802HU3	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	E.coli
CSB-EP002802HU2	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	E.coli
CSB-YP002802HU3	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	Yeast
CSB-YP002802HU3b0	Recombinant Human Bromodomain-containing protein 4 (BRD4), partial	Yeast

BRD4 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP887677MO CSB-EP887677MO CSB-BP887677MO CSB-MP887677MO CSB-EP887677MO-B	Recombinant Mouse Bromodomain-containing protein 4 (Brd4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli