BRAP
BRAP，全稱為BRCA1相關(guān)蛋白（BRCA1-associated protein），也被稱為BAP2、IMP或RNF52。該蛋白屬于RING指蛋白家族，具有一個(gè)保守的RING結(jié)構(gòu)域，參與蛋白質(zhì)間的相互作用，尤其是在泛素化和DNA損傷修復(fù)過程中。BRAP蛋白主要在細(xì)胞核內(nèi)表達(dá)，與BRCA1等蛋白結(jié)合，影響腫瘤抑制功能，在維持基因組穩(wěn)定性中起關(guān)鍵作用。
BRAP蛋白的異常表達(dá)與多種疾病相關(guān)，如心血管疾病和自身免疫性疾病。在腫瘤領(lǐng)域，BRAP蛋白的高表達(dá)與基因組不穩(wěn)定性增加有關(guān)，從而提升癌癥風(fēng)險(xiǎn)，如乳腺癌、卵巢癌等。基于BRAP蛋白的特性，研究人員正在探索其作為潛在治療靶點(diǎn)的可能性。目前，已有研究顯示BRAP蛋白在食管癌的侵襲轉(zhuǎn)移中發(fā)揮重要作用，其高表達(dá)能夠促進(jìn)食管癌細(xì)胞的遷移侵襲能力，并降低患者的生存時(shí)間。此外，BRAP蛋白也被認(rèn)為是新冠肺炎抗炎藥物研發(fā)的潛在靶點(diǎn)之一。
熱銷產(chǎn)品
Recombinant Human BRCA1-associated protein (BRAP),Partial (CSB-EP773793HU(M))
驗(yàn)證數(shù)據(jù)
-SDS.jpg)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
BRAP Antibody (CSB-PA773793LA01HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: Mouse Brain tissue lysate, Mouse Heart tissue lysate, Rat Heart tissue lysate,
All lanes: BRAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Western Blot
Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate
All lanes: BRAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
IHC image of CSB-PA773793LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
Immunofluorescence staining of Hela cell with CSB-PA773793LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
BRAP Antibodies
BRAP for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA773793LA01HU | BRAP Antibody | Human, Rat, Mouse | ELISA, WB, IHC, IF |
| CSB-PA773793LB01HU | BRAP Antibody, HRP conjugated | Human | ELISA |
| CSB-PA773793LC01HU | BRAP Antibody, FITC conjugated | Human | |
| CSB-PA773793LD01HU | BRAP Antibody, Biotin conjugated | Human | ELISA |
BRAP Proteins
BRAP Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP773793HU | Recombinant Human BRCA1-associated protein (BRAP) | Yeast |
| CSB-EP773793HU CSB-BP773793HU CSB-MP773793HU CSB-EP773793HU-B |
Recombinant Human BRCA1-associated protein (BRAP) | E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP773793HU(M) | Recombinant Human BRCA1-associated protein (BRAP),Partial | E.coli |
BRAP Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP860396MO CSB-EP860396MO CSB-BP860396MO CSB-MP860396MO CSB-EP860396MO-B |
Recombinant Mouse BRCA1-associated protein (Brap) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
