Recombinant Mouse Exostosin-1 (Ext1)

in vitro E.coli expression system

中文名稱(chēng)：

小鼠Ext1重組蛋白
貨號(hào)：

CSB-CF007899MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Ext1
Uniprot No.：

P97464
別名：

Ext1; Exostosin-1; Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Multiple exostoses protein 1 homolog
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-746
氨基酸序列

MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFPDALRPFFPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Required for the exosomal release of SDCBP, CD63 and syndecan.
基因功能參考文獻(xiàn)：
1. reactivating SHH signaling in mutant lungs rescued the tip dilation phenotype and attenuated FGF signaling. Importantly, the reduced SHH signaling activity did not appear to be caused by decreased Shh expression or protein stability; instead, biologically active form of SHH proteins were reduced in both the Ext1 mutant epithelium and surrounding wild type mesenchymal cells. PMID: 28859094
2. crucial for outflow tract formation in distinct progenitor cells and modulates FGF signaling during early heart development PMID: 26295701
3. Normal heparin sulfate levels and Ext1 activity in the stromal microenvironment and hematopoietic stem cells are required for the transplant engraftment following non-myeloablative conditioning. PMID: 25976459
4. Heterozygous loss of function of EXT1 and EXT2 results in a decreased arteriolar endothelial glycocalyx but improved flow mediated vasodilation. PMID: 25468659
5. Ext genes and heparan sulfate are needed to establish and maintain perichondrium's phenotype and border function. PMID: 23458899
6. Levels of Ext1 and HPSE influence the motility of FBJ osteosarcoma cells. PMID: 23054193
7. stromal Ext1-levels modulate tumor cell proliferation and affect the interstitial fluid pressure in a 3-D spheroid model. PMID: 22848466
8. The study indicates that formation of stereotypic exostoses requires a significant, but not complete, loss of Ext expression. PMID: 21310272
9. local Ext1 expression and heparan sulfate production are needed to maintain the phenotype and function of joint-forming cells. PMID: 21185280
10. Ext1 has a role in regulation of bone morphogenic protein signaling and skeletal defects PMID: 20404326
11. inactivation of Ext1 in a small fraction of chondrocytes is sufficient for the development of osteochondromas and other skeletal defects associated with multiple hereditary exostoses PMID: 20534475
12. for a model of multiple osteochondromas, we created the chimeric tissue genotype of somatic loss of heterozygosity, by conditionally inactivating Ext1 in chondrocytes PMID: 20080592
13. loss of heterozygosity is required for osteochondroma formation and a proliferating chondrocyte is suggested as its cell of origin PMID: 20133829
14. Induction of EXT1 knockdown in vivo dramatically suppresses the growth of bone marrow localized myeloma. PMID: 19965677
15. Data suggest that the length of the heparan sulfate (HS) chains is a critical determinant of HS-protein interactions and emphasize the essential role of EXT1 in providing specific binding sites for growth factors and extracellular matrix proteins. PMID: 19850926
16. EXT1 peaks during early postnatal period in the cerebrum and around birth in the cerebellum. EXT1 was localized primarily in the neuroepithelial cells surrounding the lateral ventricles, the mesencephalic vesicle, and the fourth ventricle. PMID: 12644256
17. Ext1 may have a role in normal mouse development PMID: 15161920
18. Reduced heparan sulfate synthesis in mice carrying a hypomorphic mutation in Ext1 results in an elevated range of Indian hedgehog (Ihh) signaling during embryonic chondrocyte differentiation. PMID: 15177029
19. Defects in EXT1 and the resulting reduction in heparan sulfate lead to enhanced Indian hedgehog diffusion causing an increase in chondrocyte proliferation and delayed hypertrophic differentiation. PMID: 15777636
20. NDST1 competes with EXT1 for binding to EXT2. PMID: 18337501
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亞細(xì)胞定位：

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein.
蛋白家族：

Glycosyltransferase 47 family
組織特異性：

Expressed in maturing chondrocytes.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:14042

STRING: 10090.ENSMUSP00000076505

UniGene: Mm.309395