VSV-G-Tag Monoclonal Antibody
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中文名稱：VSV-G-Tag鼠單克隆抗體
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貨號：CSB-MA000161
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規(guī)格：￥1090
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圖片：
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IP antibody use: 5µg VSV-G Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with VSV-G-tag fusion protein
Lane 3: IP (untransfted 293 + anti-VSV-G mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-VSV-G mAb + Protein G agarose)
Lane 6: IP (transfected 293 + Protein G)
Lane 7: Recombinant protein (E.coli) -
IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G)
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1µg VSV-G fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA000161 VSV-G-Tag單克隆抗體是針對水皰性口炎病毒糖蛋白標(biāo)簽（VSV-G-Tag）研發(fā)的高特異性科研試劑，適用于病毒載體構(gòu)建、基因治療及分子生物學(xué)研究領(lǐng)域。VSV-G作為病毒包膜的關(guān)鍵組分，廣泛用于重組慢病毒、狂犬病毒等載體的包裝系統(tǒng)，其標(biāo)簽特性可高效追蹤外源基因表達(dá)及病毒顆粒的組裝過程。該抗體采用雜交瘤技術(shù)制備，經(jīng)ELISA、Western Blot、免疫熒光（IF）和免疫沉淀（IP）等多平臺驗證，能穩(wěn)定識別天然蛋白與重組表達(dá)的VSV-G-Tag，物種兼容性覆蓋哺乳動物、昆蟲等多種表達(dá)系統(tǒng)。研究人員可將其應(yīng)用于病毒載體滴度檢測、融合蛋白表達(dá)驗證、宿主細(xì)胞相互作用分析等實驗，尤其在活細(xì)胞成像、蛋白定位及復(fù)合物分離等場景表現(xiàn)優(yōu)異。作為高性價比的科研工具，本產(chǎn)品為基因遞送系統(tǒng)開發(fā)、病毒學(xué)機(jī)制研究及生物標(biāo)記物篩選提供可靠支持，相關(guān)關(guān)鍵詞包括VSV-G抗體、病毒載體檢測、重組蛋白分析、免疫沉淀試劑。
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：Synthetic Peptide
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標(biāo)記方式：Non-conjugated
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抗體亞型：IgG
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克隆號：14H6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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