Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A (ATXA)

中文名稱(chēng)：

Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A(ATXA)
貨號(hào)：

CSB-YP360428VFO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A(ATXA)
貨號(hào)：

CSB-EP360428VFO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A(ATXA)
貨號(hào)：

CSB-EP360428VFO-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A(ATXA)
貨號(hào)：

CSB-BP360428VFO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin A(ATXA)
貨號(hào)：

CSB-MP360428VFO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

P00626
別名：

Basic phospholipase A2 ammodytoxin A; AtxA; svPLA2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase
種屬：

Vipera ammodytes ammodytes (Western sand viper)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

17-138
氨基酸序列

SLLE FGMMILGETG KNPLTSYSFY GCYCGVGGKG TPKDATDRCC FVHDCCYGNL PDCSPKTDRY KYHRENGAIV CGKGTSCENR ICECDRAAAI CFRKNLKTYN YIYRNYPDFL CKKESEKC
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Snake venom phospholipase A2 (PLA2) that acts as a presynaptic neurotoxin, an inhibitor of blood coagulation, and has been found to bind with high affinity to intracellular proteins. The response of indirectly stimulated neuromuscular preparations to ammodytoxin (Atx) is triphasic. The first phase, the transient inhibition of the acetylcholine (ACh) release, starts soon after the addition of Atx and lasts for several minutes. This phase is probably independent of Atx enzymatic activity. The effect may be due to the specific binding of the toxin to presynaptic receptors. These receptors, called N-type receptors, are still unidentified. It is noteworthy that a neuronal isoform of the M-type PLA2 receptor (R180) has been identified as a high-affinity receptor for Atx in neuronal plasma membranes. It was demonstrated however that this receptor is not essential for expression of neurotoxicity by Atx. The second phase corresponds to an augmentation of neurotransmitter release. A peak is reached 10-20 min after exposure of the preparation to Atx and is followed by a gradual reduction. In this phase, the enzymatic activity of Atx of the mammalian is not significant. It is speculated that the increased release of neurotransmitter in this phase is induced by the interference of Atx with voltage-gated potassium channels. Measurements of ionic currents showed however that voltage-gated potassium channels are not affected by Atx. The third phase of the response of neuromuscular preparations to Atx, which corresponds to a complete and irreversible paralysis, is clearly dependent on the hydrolytic activity of the toxin. In addition to its presynaptic neurotoxicity, Atx shows an anticoagulant activity by binding with high affinity to activated coagulation factor X (F10) thus inhibiting the formation of the prothrombinase complex (FX/FV) and its activity (IC(50) is 20 nM). Surprisingly, Atx was discovered to bind intracellular proteins such as calmodulin (CaM) (IC(50) is 6 nM), 14-3-3 proteins gamma (YWHAG) and epsilon (YWHAE) (by similarity with AtxC), as well as R25 (by similarity with AtxC), a mitochondrial integral membrane protein found in cerebral cortex. These findings raised a doubt about the dogma of the exclusively extracellular action of PLA2s, defended by the potential instability of these molecules in the reducing environment of the eukaryotic cytosol coupled with their possible inability to act as enzymes in this cellular compartment, due to too low concentration of calcium ions. This hypothesis was challenged efficiently by demonstrating the internalization of AtxA into a culture cells, but still remains to be directly demonstrated in vivo. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
亞細(xì)胞定位：

Secreted. Host cytoplasm, host cytosol.
蛋白家族：

Phospholipase A2 family, Group II subfamily, D49 sub-subfamily
組織特異性：

Expressed by the venom gland.