Recombinant Staphylococcus aureus Enterotoxin type B (entB)

^{In
Stock}

文獻引用 (2)

中文名稱：

金黃色釀膿葡萄球菌entB重組蛋白
品名簡稱：

Recombinant Staphylococcus aureus entB protein
貨號：

CSB-YP360703FKZ
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

entB
Uniprot No.：

P01552
別名：

entBEnterotoxin type B; SEB
種屬：

Staphylococcus aureus
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

30.4kDa
表達區域：

28-266aa
氨基酸序列

ESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTCMYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Full-length of mature Staphylococcus aureus Enterotoxin type B (entB) cDNA carrying an N-terminal 6xHis-tag was expressed in the yeast cells. The resulting protein is the recombinant fusion protein consisting of 28-266aa of Staphylococcus aureus entB. The SDS-PAGE showed an about 28-33 kDa molecular mass of this protein and determined its purity of over 90%. In-stock entB proteins are offered now. In addition to producing specific anti-entB antibodies, this recombinant entB protein may also find uses in the microbiology.

Staphylococcus aureus-secreted exotoxin entB is one of the most potent bacterial superantigens (SAgs) that exerts toxic effects upon the immune system, inducing cytokine release and eliciting inflammation. EntB mainly targets T-cell receptors (TCRs) on the T cells and major histocompatibility complex (MHC) class II molecules on Ag-presenting cells (APCs), forming a ternary complex between specific Vβ chains and MHC class II molecules through cross-link. It is water-soluble, heat unstable, and resistant to the proteolytic enzyme, including trypsin, papain, and pepsin. And it also possesses emetic activity. EntB can cause multi-organ system failure and death at low concentrations. The involvement of entB in the pathogenesis of some diseases such as atopic dermatitis, asthma, and chronic rhinitis, has been documented.
Datasheet & COA：

Please contact us to get it.

引用文獻

High-sensitivity lateral flow immunoassay strip based on surface-enhanced raman scattering and detection method using the same Jaebum CHOO, et al,/,2019
Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B Hwang J. et al,Nanoscale,2016

產品評價

靶點詳情

功能：

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of proinflammatory cytokines. Causes also the intoxication staphylococcal food poisoning syndrome.
亞細胞定位：

Secreted.
蛋白家族：

Staphylococcal/streptococcal toxin family