Recombinant Schizosaccharomyces pombe Division mal foutue 1 protein (mid1), partial

中文名稱：

龐貝裂殖酵母mid1重組蛋白
貨號：

CSB-YP302911SXV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

龐貝裂殖酵母mid1重組蛋白
貨號：

CSB-EP302911SXV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

龐貝裂殖酵母mid1重組蛋白
貨號：

CSB-EP302911SXV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

龐貝裂殖酵母mid1重組蛋白
貨號：

CSB-BP302911SXV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

龐貝裂殖酵母mid1重組蛋白
貨號：

CSB-MP302911SXV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

mid1
Uniprot No.：

P78953
別名：

mid1; dmf1; SPCC4B3.15; Division mal foutue 1 protein
種屬：

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Scaffold protein that anchors the contractile ring (CR) at the cell equator during cytokinesis. At the onset of mitosis, membrane-bound oligomers of mid1 assemble recruitment platforms for cytokinetic ring components at the medial cortex and stabilize the ring position during its compaction. Recruits dephosphorylated myo2, but also rng2, clp1 and cdc15 to nodes and to place cytokinetic nodes around the cell equator the medial cortex to promote the ring assembly in cooperation with F-actin. Necessary to stabilize the mitotic spindle perpendicular to the axis of cell division. Recruits also the cdr2 kinase to the CR.; In the nucleus, binds to the promoter regions of M-G1 transcribed genes to negatively regulate their expression.
基因功能參考文獻：
1. Unlike in wild-type cells, actomyosin rings in cells rewired to divide medially in the absence of Mid1p assemble late in anaphase. The rewiring process affects the ability of the actomyosin ring to track the nucleus upon perturbation of nuclear position. PMID: 25176634
2. Pom1 inhibits Cdr2 interaction with Mid1, reducing its clustering ability. PMID: 24982431
3. An overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells, is provided. PMID: 22888038
4. Orderly assembly of the contractile ring in wild-type cells depends on Mid1p. PMID: 22918943
5. Residues 1-578 of Mid1p assemble node components myosin-II, Rng2p, and Cdc15p, and the insoluble domain facilitates the normal, efficient condensation of nodes into rings. PMID: 22918954
6. Blt1 acts as a scaffolding protein for precursors of the cytokinetic ring, and Blt1 and Mid1 provide overlapping membrane anchors for proper division plane positioning. PMID: 23149940
7. The N-terminus of Mid1 physically interacts with cytokinesis node proteins. PMID: 22427686
8. Clp1p and Mid1p form links between cell cycle progression and gene expression at cytokinesis in fission yeast. PMID: 21512312
9. Mid1p recruits Rng2p to cortical nodes at the cell-division site. PMID: 21376595
10. Live microscopy of cells expressing Cdc12p fluorescent fusion proteins shows for the first time that Cdc12p localizes to a broad band of 30-50 dynamic nodes, where actin filaments are nucleated in random directions. PMID: 19864459
11. Evidence indicates that Clp1/Flp1 must interact with the Mid1 scaffold to ensure the fidelity of Schizosaccharomyces pombe cytokinesis. PMID: 18378776
12. Data show that Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis. PMID: 19056897
13. A mechanism that is independent of cortical nodes is sufficient for the assembly of normal actomyosin rings. PMID: 19075108
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亞細胞定位：

Nucleus. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.
數據庫鏈接：

KEGG: spo:SPCC4B3.15

STRING: 4896.SPCC4B3.15.1