Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase ROK1 (ROK1), partial

中文名稱：

釀酒酵母ROK1重組蛋白
貨號(hào)：

CSB-YP338325SVG
說明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

釀酒酵母ROK1重組蛋白
貨號(hào)：

CSB-EP338325SVG
說明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

釀酒酵母ROK1重組蛋白
貨號(hào)：

CSB-EP338325SVG-B
說明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母ROK1重組蛋白
貨號(hào)：

CSB-BP338325SVG
說明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

釀酒酵母ROK1重組蛋白
貨號(hào)：

CSB-MP338325SVG
說明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

ROK1
Uniprot No.：

P45818
別名：

ROK1; YGL171W; G1651; ATP-dependent RNA helicase ROK1; EC 3.6.4.13; Rescuer of KEM1 protein 1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.
基因功能參考文獻(xiàn)：
1. Data show that ATP-bound DEAD-box protein Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 protein binding to 40S ribosomes. PMID: 27280440
2. Rrp5 provides specificity to the otherwise nonspecific biochemical activities of the Rok1 DEAD-domain. PMID: 23630256
3. Immunoprecipitation assays demonstrate the association of Rok1p with 35S pre-rRNA in pre-ribosomes. PMID: 15523097
4. Rok1 was required specifically for the release of snR30. PMID: 18833290
亞細(xì)胞定位：

Nucleus, nucleolus.
蛋白家族：

DEAD box helicase family, DDX52/ROK1 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:YGL171W

STRING: 4932.YGL171W