Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase DHH1 (DHH1), partial

中文名稱：

釀酒酵母DHH1重組蛋白
貨號：

CSB-YP327987SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母DHH1重組蛋白
貨號：

CSB-EP327987SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母DHH1重組蛋白
貨號：

CSB-EP327987SVG-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母DHH1重組蛋白
貨號：

CSB-BP327987SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母DHH1重組蛋白
貨號：

CSB-MP327987SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

DHH1
Uniprot No.：

P39517
別名：

DHH1; YDL160CATP-dependent RNA helicase DHH1; EC 3.6.4.13; DExD/H-box helicase 1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

ATP-dependent RNA helicase involved in mRNA turnover, and more specifically in mRNA decapping by activating the decapping enzyme DCP1. Is involved in G1/S DNA-damage checkpoint recovery, probably through the regulation of the translational status of a subset of mRNAs. May also have a role in translation and mRNA nuclear export. Required for sporulation. Blocks autophagy in nutrient-rich conditions by, at least partly, binding and repressing the expression of a set of ATG genes, including ATG3, ATG7, ATG8, ATG19, ATG20, ATG22 and SNX4/ATG24.
基因功能參考文獻：
1. All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site; second, they are directly bound by Dhh1 with a specific binding distribution; and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step PMID: 27821408
2. Here, the authors show that in budding yeast, mutations in the DEAD-box ATPase Dhh1 that prevent ATP hydrolysis, or that affect the interaction between Dhh1 and Not1, the central scaffold of the CCR4-NOT complex and an activator of the Dhh1 ATPase, prevent processing bodies disassembly in vivo. PMID: 27692063
3. findings show that the mutations in ATPase A and B motif (K96R, D195A) affected the growth at non-permissive temperature, the mating processes including the Ste12 expression, and the P-body accumulation PMID: 28455591
4. These results suggest that after localization to the bud tip, a portion of the localized ASH1 mRNA becomes translationally inactive because of binding of Dhh1 and Puf6 to the 5'- and 3'-UTRs of ASH1 mRNA. PMID: 28450395
5. Data indicate that RNA helicase RCK family member Dhh1 and the mRNA decay pathway coordinately repress the autophagy gene expression. PMID: 26098573
6. Dhh1 activates decapping in response to impaired ribosome elongation. PMID: 22719226
7. Pull-down experiments reveal that the intracellular binding partners of Dhh1 change as cells undergo the diauxic growth shift. PMID: 21712243
8. Results place Dhh1 at the interface of translation and decay controlling whether an mRNA is translated, stored, or decayed. PMID: 21844211
9. Pbp1 and Dhh1 over-expression inhibits cell growth, and for Dhh1, leads to the accumulation of stress granules PMID: 20368989
10. DHH1 has a role in G1/S DNA-damage checkpoint recovery PMID: 15166134
11. The Dhh1p is as functioning as translational repressors and facilitators of P body formation. PMID: 16179257
12. Caf20 and Dhh1 control filamentation by regulating STE12 translation. PMID: 17041186
13. We propose that Dhh1 and Caf20 regulate the Ste12 protein expression and the Ste12 protein level is associated with P-body formation during mating. PMID: 18182159
顯示更多

收起更多
亞細胞定位：

Cytoplasm, P-body.
蛋白家族：

DEAD box helicase family, DDX6/DHH1 subfamily
數(shù)據(jù)庫鏈接：

KEGG: sce:YDL160C

STRING: 4932.YDL160C