Recombinant Rotavirus X Outer capsid protein VP4, partial

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中文名稱：

Recombinant Rotavirus X Outer capsid protein VP4,partial
貨號：

CSB-YP434929RIR
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

N/A
Uniprot No.：

A9Q1L0
別名：

; Outer capsid protein VP4; Hemagglutinin) [Cleaved into: Outer capsid protein VP8*; Outer capsid protein VP5*]
種屬：

Rotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
蛋白長度：

Partial
來源：

Yeast
分子量：

30.8kDa
表達區域：

1-249aa
氨基酸序列

MSLRSLLITTEAVGETTQTSDHQTSFSTRTYNEINDRPSLRVEKDGEKAYCFKNLDPVRYDTRMGEYPFDYGGQSTENNQLQFDLFTKDLMADTDIGLSDDVRDDLKRQIKEYYQQGYRAIFLIRPQNQEQQYIASYSSTNLNFTSQLSVGVNLSVLNKIQENKLHIYSTQPHIPSVGCEMITKIFRTDVDNENSLINYSVPVTVTISVTKATFEDTFVWNQNNDYPNMNYKDLIPAVTKNSIYHDVKR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Recombinant Rotavirus X Outer capsid protein VP4 protein was made through genetic engineering. By putting the Outer capsid protein VP4 gene into the genetic material of Yeast cell, the Yeast could be used as factories or producers to make the desired Outer capsid protein VP4 protein for research uses. The expression region of this protein is at 1-249aa. N-terminal 6xHis tag was used in the expression process. The purity is 85%+ determined by SDS-PAGE.
VP4 is a small (7.5 kDa), linear, hydrophobic peptide that resides on each triangular facet of the capsid interior. VP4 and its precursor VP0 are myristoylated at the NH2 terminus upon virus assembly. Receptor binding induces conformational changes in the virus, including translocation of VP4 and the NH2-terminal region of VP1 to the exterior viral surface. VP1 tethers the particle to the membrane, while myristoylated (Myr) VP4 (MyrVP4) forms a multimeric membrane pore, allowing extrusion of viral RNA genome into the cytoplasm. VP4 is directly involved in mediating membrane penetration during cell entry. Studies utilizing recombinant His-tagged VP4 have shown that VP4 interacts with membranes to make them permeable by formation of multimeric, size-selective membrane pores with properties consistent with the transport of viral genome through the membrane.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. It is subsequently lost, together with VP7, following virus entry into the host cell. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.; Forms the spike 'foot' and 'body' and acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. During entry, the part of VP5* that protrudes from the virus folds back on itself and reorganizes from a local dimer to a trimer. This reorganization may be linked to membrane penetration.; Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. It is the viral hemagglutinin and an important target of neutralizing antibodies.
亞細胞定位：

[Outer capsid protein VP4]: Virion. Host rough endoplasmic reticulum. Host cell membrane. Host endoplasmic reticulum-Golgi intermediate compartment.
蛋白家族：

Rotavirus VP4 family