Recombinant Rhodobacter sphaeroides Malyl-CoA lyase (mcl1)

中文名稱：

球形紅桿菌mcl1重組蛋白
貨號：

CSB-YP667039RLF
規格：
來源：

Yeast
其他：

中文名稱：

球形紅桿菌mcl1重組蛋白
貨號：

CSB-EP667039RLF
規格：
來源：

E.coli
其他：

中文名稱：

球形紅桿菌mcl1重組蛋白
貨號：

CSB-EP667039RLF-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

球形紅桿菌mcl1重組蛋白
貨號：

CSB-BP667039RLF
規格：
來源：

Baculovirus
其他：

中文名稱：

球形紅桿菌mcl1重組蛋白
貨號：

CSB-MP667039RLF
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

mcl1
Uniprot No.：

Q3J5L6
別名：

mcl1; RHOS4_03500; RSP_1771L-malyl-CoA/beta-methylmalyl-CoA lyase; EC 4.1.3.24,; 3S)-malyl-CoA/beta-methylmalyl-CoA lyase,; S)-citramalyl-CoA lyase; EC 4.1.3.25
種屬：

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-318
氨基酸序列

SFRLQPAPP ARPNRCQLFG PGSRPALFEK MAASAADVIN LDLEDSVAPD DKAQARANII EAINGLDWGR KYLSVRINGL DTPFWYRDVV DLLEQAGDRL DQIMIPKVGC AADVYAVDAL VTAIERAKGR TKPLSFEVII ESAAGIAHVE EIAASSPRLQ AMSLGAADFA ASMGMQTTGI GGTQENYYML HDGQKHWSDP WHWAQAAIVA ACRTHGILPV DGPFGDFSDD EGFRAQARRS ATLGMVGKWA IHPKQVALAN EVFTPSETAV TEAREILAAM DAAKARGEGA TVYKGRLVDI ASIKQAEVIV RQAEMISA
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in the ethylmalonyl-CoA pathway for acetate assimilation. Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to yield beta-methylmalyl-CoA. It is also able to catalyze the cleavage of (S)-citramalyl-CoA to yield acetyl-CoA and pyruvate, although this reaction is not involved in the ethylmalonyl-CoA pathway.
基因功能參考文獻：
1. study reports the crystal structures of Malyl-CoA lyase (MCL) from 2 different phylogenetic subgroups in apo-and substrate-bound forms; both the C. aurantiacus and the R. sphaeroides MCL contain elaborations on the canonical beta8/alpha8 TIM barrel fold and form hexameric assemblies PMID: 24206647
蛋白家族：

HpcH/HpaI aldolase family
數據庫鏈接：

KEGG: rsp:RSP_1771

STRING: 272943.RSP_1771