Recombinant Rat Serine/threonine-protein kinase mTOR (Mtor), partial

中文名稱：

大鼠Mtor重組蛋白
貨號：

CSB-YP008968RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Mtor重組蛋白
貨號：

CSB-EP008968RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Mtor重組蛋白
貨號：

CSB-EP008968RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Mtor重組蛋白
貨號：

CSB-BP008968RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Mtor重組蛋白
貨號：

CSB-MP008968RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mtor
Uniprot No.：

P42346
別名：

Mtor; Frap1; Raft1; Serine/threonine-protein kinase mTOR; EC 2.7.11.1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin target protein 1; RAPT1
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. This also includes mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3: in the presence of nutrients, mediates phosphorylation of TFEB and TFE3, promoting their cytosolic retention and inactivation. Upon starvation or lysosomal stress, inhibition of mTORC1 induces dephosphorylation and nuclear translocation of TFEB and TFE3, promoting their transcription factor activity. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions. Prevents autophagy by mediating phosphorylation of AMBRA1, thereby inhibiting AMBRA1 ability to mediate ubiquitination of ULK1 and interaction between AMBRA1 and PPP2CA. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-421'. Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms. Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks. Phosphorylates SQSTM1, promoting interaction between SQSTM1 and KEAP1 and subsequent inactivation of the BCR(KEAP1) complex.
基因功能參考文獻：
1. modulation of Ser758 ULK1 phosphorylation following LRRK2 inhibition is decoupled from the repression of autophagosome biogenesis and independent of mTOR complex 1 activity. PMID: 29563162
2. Inhibition of the mTOR pathway has cardioprotective effects on myocardial dysfunction during sepsis induced by cecal ligation and puncture, which is partly mediated through autophagy PMID: 30050390
3. Dysregulation of PI3K-Akt-mTOR pathway in the brain of streptozotocin-induced type 2 diabetes mellitus rats. PMID: 30041644
4. Activation of the PI3K/Akt/mTOR pathway contributed to the inhibitory effect of hesperidin on excessive autophagy. PMID: 30066841
5. the protective effects of liraglutide may be associated with increased mTOR expression via activation of the AMPK and PI3K/Akt signaling pathways. PMID: 29916537
6. mTOR is a direct interacting partner of Trx1 in the heart. Trx1 protects cardiomyocytes against oxidative stress by reducing mTOR at Cys-1483, thereby preserving the activity of mTOR and inhibiting cell death. PMID: 28939765
7. Cardiac progenitor cellderived exosomes promoted H9C2 cell growth via the activation of Akt/mTOR. PMID: 29786755
8. these findings suggested that resveratrol promoted functional recovery and inhibited neuroinflammation through the activation of autophagy mediated by the AMPK/mTOR pathway following spinal cord injury. PMID: 29956767
9. It's overactivation acts as a signaling hub for the formation of tau hyperphosphorylation, which contributes to the development of diabetes-associated cognitive deficit PMID: 29674181
10. The PI3K/mTOR/AKT pathway is known to regulate cell growth, survival, and proliferation and has more recently been shown to be a central mediator of HCC cell survival to heat stress. PMID: 29737948
11. that BDNF activates mTOR to up-regulate NR2B expression, which is required for inflammatory pain-related aversion in the rostral anterior cingulate cortex of rats PMID: 29353374
12. Intestinal injury of Intrauterine growth retardation fetuses caused by maternal food restriction might be due to enhanced apoptosis and autophagy via the mTOR signaling pathway. PMID: 28855439
13. Melatonin played an important and protective role in CMECs by inhibiting autophagy against IRI via the AMPK/mTOR system. PMID: 29975938
14. These results suggest that synaptic activity-derived signaling pathways act in combination with mTORC1-dependent translational control mechanisms to ultimately regulate changes in spine morphology. PMID: 29084578
15. TGFB1-induced chondrogenesis of bone marrow mesenchymal stem cells is promoted by low-intensity pulsed ultrasound through the integrin-mTOR signaling pathway. PMID: 29237506
16. characterized the phosphoproteome that is regulated by insulin as well as its key downstream kinases including Akt, mTORC1, and S6K. PMID: 28689409
17. Sevoflurane ameliorates myocardial injury by affecting the phosphorylation states of the proteins in PI3K/Akt/mTOR signaling pathway. PMID: 28198521
18. the results from this study not only establish that rapamycin-sensitive mTOR plays an important role in the RE-induced activation of protein synthesis and the induction of hypertrophy, but they also demonstrate that additional (rapamycin-sensitive mTOR-independent) mechanisms contribute to these fundamentally important events. PMID: 27502839
19. Our data provide the first evidence for a critical sequential role for mTOR-Notch3-PDGF-beta receptor signaling in the pathogenesis of chronic neonatal pulmonary hypertension. PMID: 28759157
20. Abeta increases the expression of mTOR and p-mTOR at the site of Ser2448, and the stimulation of Abeta is likely to depend on sirtuin 1, PPARgamma, and PGC-1beta pathway in regulating mTOR expression. PMID: 27631411
21. Inhibition of PI3K/AKT/mTOR signaling pathway could promote the autophagy. PMID: 28320092
22. PF inhibited autophagy at least partly through inhibiting RAGE and upregulating the level of p-mTOR to against AGEs-induced mesangial cell dysfunction. PMID: 28320103
23. Results suggest that mammalian target of rapamycin (mTOR) may act via transcription factor AP-1 (AP-1) to regulate vascular endothelial growth factor (VEGF) expression. PMID: 27458160
24. Taken together, we provide evidence that UC-MSCs therapy can successfully alleviate IC in a preclinical animal Model and cell model by alleviating inflammation, promoting proliferation and inhibiting apoptosis. PMID: 29137981
25. Results suggest that mammalian target of rapamycin (mTOR) signaling is sensitive to exercise, but subtle differences exist depending on exercise controllability. Increases in mTOR signaling could contribute to the beneficial effects of exercise on cognitive function and mental health. PMID: 28130174
26. the CAMKK2-MTOR pathway has a role in store-operated calcium entry-activated autophagy protection of endothelial progenitor cell proliferation in ox-LDL exposure PMID: 27791458
27. Intraplantar injection of formalin was associated with significant activation of mTOR, as well as its downstream effector p70 ribosomal S6 protein (p70S6K) in the anterior cingulate cortex. The inhibition of mTOR activation with rapamycin disrupted pain-related aversion; however, this inhibition did not affect formalin-induced spontaneous nociceptive behaviors in rats. PMID: 27163752
28. Our findings provide evidence of sustained activation of autophagy in RV remodeling of MCT induced PH model, while pro-autophagic signaling pathways varied depending on the phase. PMID: 28340591
29. Silk fibroin peptide (SF16) hydrogel promotes PC12 growth/adhesion via mTOR/cadherin signaling pathway. PMID: 28213972
30. Celastrol attenuates cadmium chloride-induced neuronal apoptosis via inhibition of Ca(2+) -CaMKII-dependent Akt/mTOR signaling pathway. PMID: 27891586
31. Probucol induces autophagy by inhibiting the mTOR signaling pathway and inhibiting neural cell apoptosis after spinal cord injury. PMID: 27223630
32. Caloric restriction (CR) and metformin treatment activated AMPK-mTOR signaling in the kidneys of old rats. PMID: 28147330
33. This study demonstrates that one of the critical mechanisms underlying curcumin inhibiting heat-induced apoptosis is through suppressing NADPH Oxidase 2 and activating the Akt/mTOR signaling pathway in bronchial epithelial cells. PMID: 28478460
34. Collectively, our findings suggest for the first time the possible protective mechanism of neferine, which is crucial for its further development as a potential therapeutic agent for cisplatin-induced nephrotoxicity. PMID: 28161641
35. these evidence suggested that PU restored the impaired autophagic flux in Pb-treated rPT cells partly by activating autophagy via AMPK/mTOR-mediated signaling pathway. PMID: 27762461
36. Mammalian target of rapamycin complex 2 regulates inflammatory response to stress PMID: 22899279
37. The functional relationship between LKB1 and mTOR in regulating adipogenesis PMID: 27235551
38. Findings suggested that the rapamycin and AZD8055 could attenuate the development of early brain injury in this subarachnoid hemorrhage model, possibly through inhibiting the activation of microglia by mTOR pathway PMID: 27423593
39. In conclusion, miRNA-221 exerts cytoprotective effects in hypoxia-reoxygenation injury in association with alterations in autophagic cell injury. Mir-221 may constitute is a novel therapeutic target in the treatment of cardiac I/R injury. PMID: 27105917
40. Demonstrate a potential role for cardiac mTOR in the pathophysiology of cirrhotic cardiomyopathy. PMID: 27217700
41. n-3 Fatty acids modulate the mRNA expression of the Nlrp3 inflammasome and Mtor in the liver of rats fed with high-fat or high-fat/fructose diets. PMID: 27367537
42. Suggest that 4-nonylphenol stimulates Sertoli cell autophagy and inhibits mTOR-p70S6K/4EBP1 activity through AMPK activation. PMID: 28041982
43. Macrophage migration inhibitory factor exerts a permissive role in protecting bone marrowderived mesenchymal stem cells from apoptosis by regulation of autophagy via the AMPK/mTOR signaling pathway PMID: 26847932
44. mTOR plays an important role in spermatogenesis by regulating p70s6k activation; 4e-bp1 is either directly or indirectly regulated by PI3K PMID: 26706460
45. mTOR kinase inhibitor pp242 causes mitophagy terminated by apoptotic cell death in E1A-Ras transformed cells. PMID: 26636543
46. Suggest miR-96 could promote myocardial hypertrophy by inhibiting mTOR activity. PMID: 26823769
47. aging does not result in increased mTOR signaling in most tissues and suggest that rapamycin does not promote lifespan by reversing or blunting such an effect PMID: 26695882
48. Data suggest that reactive oxygen species induce apoptosis in osteoblasts via in-activation of Akt (protein kinase B) and down-regulation Mtor (mammalian target of rapamycin) signaling; thus, oxidative stress may be instrumental in bone resorption. PMID: 23788393
49. Data provide evidence for the role of mammalian target of rapamycin (mTOR) in the rostral ventromedial medulla in nerve injury-induced neuropathic pain, indicating a novel mechanism of mTOR inhibitor-induced analgesia. PMID: 26770837
50. Study suggests that activation of the mTOR pathway is essential for the effects of corticosterone on synaptic trapping of AMPARs and, possibly as a consequence, fearful memory formation PMID: 26572647
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亞細胞定位：

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm. Nucleus, PML body. Lysosome membrane. Cytoplasmic vesicle, phagosome.
蛋白家族：

PI3/PI4-kinase family
數據庫鏈接：

KEGG: rno:56718

STRING: 10116.ENSRNOP00000014167

UniGene: Rn.11008