Recombinant Rat Serine/threonine-protein kinase PLK2 (Plk2)

中文名稱：

大鼠Plk2重組蛋白
貨號：

CSB-YP882590RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Plk2重組蛋白
貨號：

CSB-EP882590RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Plk2重組蛋白
貨號：

CSB-EP882590RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Plk2重組蛋白
貨號：

CSB-BP882590RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Plk2重組蛋白
貨號：

CSB-MP882590RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Plk2
Uniprot No.：

Q9R012
別名：

Plk2; Snk; Serine/threonine-protein kinase PLK2; EC 2.7.11.21; Polo-like kinase 2; PLK-2; Serine/threonine-protein kinase SNK; Serum-inducible kinase
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-682
氨基酸序列

MELLRTITYQ PAAGTKMCEQ ALGKACGGDS KKKRPQQPSE DGQSQAQVTP AAPHHHHHHS HSGPEISRII VDPTTGKRYC RGKVLGKGGF AKCYEMTDLT NNKVYAAKII PHSRVAKPHQ REKIDKEIEL HRILHHKHVV QFYHYFEDKE NIYILLEYCS RRSMAHILKA RKVLTEPEVR YYLRQIVSGL KYLHEQEILH RDLKLGNFFI NEAMELKVGD FGLAARLEPL EHRRRTICGT PNYLSPEVLN KQGHGCESDI WALGCVMYTM LLGRPPFETT NLKETYRCIR EARYTMPSSL LAPAKHLIAS MLSKNPEDRP SLDDIIRHDF FLQGFTPDRL SSSCCHTVPD FHLSSPAKNF FKKAAAALFG GKKDKARYND THNKVSKEDE DIYKLRHDLK KTSITQQPSK HRTDEELQPP PTTFAKSGTS AVENKQQIGD AIRMIVRGTL GSCSSSSECL EDSTMGSVAD TVARVLRGCL ENMPEADCIP KEQLSTSFQW VTKWVDYSNK YGFGYQLSDH TVGVLFNNGA HMSLLPDKKT VHYYAELGQC SVFPATDAPE QFISQVTVLK YFSHYMEENL MDGGDLPSVT DIRRPRLYLL QWLKSDKALM MLFNDGTFQV NFYHDHTKII ICNQNEEYLL TYINEDRIST TFRLTTLLMS GCSLELKHRM EYALNMLLQR CN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.
基因功能參考文獻：
1. Plk2 acts as coordinator of cell proliferation and early lineage commitment in cardiac progenitor cells downstream of Yes-associated protein 1. PMID: 29066438
2. PLK2, functional kinase and polo-box domains were required for interaction with PFV Gag. Fluorescently-tagged PFV Gag, through its chromatin tethering function, selectively relocalized ectopically expressed eGFP-tagged PLK proteins to mitotic chromosomes i PMID: 27579920
3. Prenatal stress increased Snk Polo-like kinase 2 in thr hippocampus of the offspring at adulthood in rats PMID: 23850969
4. In a rat model of Parkinson disease, PLK2 overexpression reduces alpha-syn levels and suppresses alpha-syn-induced toxicity and motor deficits. PMID: 23983262
5. PLK2 inhibition is a tractable CNS pharmacological target that does not cause genotoxicity at doses and exposures that engage the target in the sensory retina. PMID: 23466428
6. Mammalian target of rapamycin complex 1 activation negatively regulates Polo-like kinase 2-mediated homeostatic compensation following neonatal seizures. PMID: 23479645
7. Serum inducible kinase acts downstream of extracellular signal-regulated kinase activation in immature cortical neurons undergoing morphogenesis and is necessary for brain-derived neurotrophic factor -promoted dendrite growth and branching. PMID: 21691298
8. The data of this study demonistrated that heightened synaptic activity, acting through Plk2, leads to homeostatic decreases in surface AMPAR expression via the direct dissociation of NSF from GluA2. PMID: 20802490
9. the SNK-SPAR pathway may possibly play a crucial role in Amyloid beta-induced excitotoxic damage in the central nervous system by regulating synaptic stability. PMID: 20146300
10. induction in hippocampal neurons eliminated SPAR protein, depleted PSD-95 and Bassoon clusters & caused loss of mature dendritic spines; results implicate SNK as a mediator of activity-dependent change in the molecular composition & morphology of synapses PMID: 14576440
11. is a novel target of ERRgamma, and their interaction is crucial for cancer cell proliferation PMID: 17706602
12. Thus, Plk2 function is required to prevent escalating potentiation and maintain synapses in a plastic state during epileptiform activity in hippocampal slice cultures. PMID: 18579731
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, dendrite.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
數據庫鏈接：

KEGG: rno:83722

STRING: 10116.ENSRNOP00000016768

UniGene: Rn.12100