Recombinant Rat Nuclear receptor subfamily 1 group D member 1 (Nr1d1)

中文名稱：

大鼠Nr1d1重組蛋白
貨號(hào)：

CSB-YP730905RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

大鼠Nr1d1重組蛋白
貨號(hào)：

CSB-EP730905RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

大鼠Nr1d1重組蛋白
貨號(hào)：

CSB-EP730905RA-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Nr1d1重組蛋白
貨號(hào)：

CSB-BP730905RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

大鼠Nr1d1重組蛋白
貨號(hào)：

CSB-MP730905RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nr1d1
Uniprot No.：

Q63503
別名：

Nr1d1; Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-615
氨基酸序列

MTTLDSNNNT GGVITYIGSS GSSPSRTSPE SLYSDSSNGS FQSLTQGCPT YFPPSPTGSL TQDPARSFGT VPPSLSDDSS PSSASSSSSS SSSSFYNGSP PGSLQVAMED SSRVSPSKGT SNITKLNGMV LLCKVCGDVA SGFHYGVHAC EGCKGFFRRS IQQNIQYKRC LKNENCSIVR INRNRCQQCR FKKCLSVGMS RDAVRFGRIP KREKQRMLAE MQNAMNLANN QLSSLCPLET SPAPHPTSGS VGPSPPPAPA PTPLVGFSQF PQQLTPPRSP SPEPTVEDVI SQVARAHREI FTYAHDKLGT SPGNFNANHA SGSPPATTPQ CWESQGCPST PNDNNLLAAQ RHNEALNGLR QGPSSYPPTW PSGPAHHSCH QPNSNGHRLC PTHVYSAPEG KAPANGLRQG NTKNVLLACP MNMYPHGRSG RTVQEIWEDF SMSFTPAVRE VVEFAKHIPG FRDLSQHDQV TLLKAGTFEV LMVRFASLFN VKDQTVMFLS RTTYSLQELG AMGMGDLLNA MFDFSEKLNS LALTEEELGL FTAVVLVSAD RSGMENSASV EQLQKTLLRA LRALVLKNRP SETSRFTKLL LKLPDLRTLN NMHSEKLLSF RVDAQ
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and regulates the levels of its ligand heme by repressing the expression of PPARGC1A, a potent inducer of heme synthesis. Regulates lipid metabolism by repressing the expression of APOC3 and by influencing the activity of sterol response element binding proteins (SREBPs); represses INSIG2 which interferes with the proteolytic activation of SREBPs which in turn govern the rhythmic expression of enzymes with key functions in sterol and fatty acid synthesis. Regulates gluconeogenesis via repression of G6PC1 and PEPCK and adipocyte differentiation via repression of PPARG. Regulates glucagon release in pancreatic alpha-cells via the AMPK-NAMPT-SIRT1 pathway and the proliferation, glucose-induced insulin secretion and expression of key lipogenic genes in pancreatic-beta cells. Positively regulates bile acid synthesis by increasing hepatic expression of CYP7A1 via repression of NR0B2 and NFIL3 which are negative regulators of CYP7A1. Modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy; controls mitochondrial biogenesis and respiration by interfering with the STK11-PRKAA1/2-SIRT1-PPARGC1A signaling pathway. Represses the expression of SERPINE1/PAI1, an important modulator of cardiovascular disease and the expression of inflammatory cytokines and chemokines in macrophages. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). Plays a role in the circadian regulation of body temperature and negatively regulates thermogenic transcriptional programs in brown adipose tissue (BAT); imposes a circadian oscillation in BAT activity, increasing body temperature when awake and depressing thermogenesis during sleep. In concert with NR2E3, regulates transcriptional networks critical for photoreceptor development and function. In addition to its activity as a repressor, can also act as a transcriptional activator. In the ovarian granulosa cells acts as a transcriptional activator of STAR which plays a role in steroid biosynthesis. In collaboration with SP1, activates GJA1 transcription in a heme-independent manner. Represses the transcription of CYP2B10, CYP4A10 and CYP4A14. Represses the transcription of CES2. Represses and regulates the circadian expression of TSHB in a NCOR1-dependent manner. Negatively regulates the protein stability of NR3C1 and influences the time-dependent subcellular distribution of NR3C1, thereby affecting its transcriptional regulatory activity. Plays a critical role in the circadian control of neutrophilic inflammation in the lung; under resting, non-stress conditions, acts as a rhythmic repressor to limit inflammatory activity whereas in the presence of inflammatory triggers undergoes ubiquitin-mediated degradation thereby relieving inhibition of the inflammatory response. Plays a key role in the circadian regulation of microglial activation and neuroinflammation; suppresses microglial activation through the NF-kappaB pathway in the central nervous system. Plays a role in the regulation of the diurnal rhythms of lipid and protein metabolism in the skeletal muscle via transcriptional repression of genes controlling lipid and amino acid metabolism in the muscle.
基因功能參考文獻(xiàn)：
1. Bone morphogenetic protein gene expression is inhibited by REV-ERBalpha which acts as a transcriptional silencer. PMID: 25588874
2. Data (including data from studies in transgenic rats) suggest attenuation of circadian clock system, especially Rev-erb-alpha, contributes to induction of prostaglandin G/H synthase 2 in endometrial stromal cells during decidualization/placentation. PMID: 25648833
3. REV-ERBalpha plays an important role in ventilation induced lung injury (VILI) and inflammation, and circadian rhythm disorder inflammation response may be a novel pathogenesis of VILI. PMID: 24355794
4. Rev-erbalpha is a novel regulator of hepatic stellate cell transdifferentation PMID: 24497272
5. type 2 diabetes amelioration in diabetic GK rats was associated with improved circadian Rev-erbalpha expression, in a comparison between the surgically treated and untreated GK rats. PMID: 23637135
6. these data are the first to show the Rev-erbalpha function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erbalpha may coordinate circadian rhythm and metabolism in rat ovaries. PMID: 22425774
7. N-nitroso-N-methylurea disrupted and dietary methylselenocysteine significantly increased the expression of RevErbaAlpha in mammary glands of Fischer 344 rats PMID: 20424134
8. ligand modulation of REV-ERBalpha function resets the peripheral circadian clock in a phasic manner PMID: 18946026
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亞細(xì)胞定位：

Nucleus. Cytoplasm. Cell projection, dendrite. Cell projection, dendritic spine.
蛋白家族：

Nuclear hormone receptor family, NR1 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:252917

STRING: 10116.ENSRNOP00000012537

UniGene: Rn.29848