Recombinant Rat Neuroendocrine convertase 1 (Pcsk1)

中文名稱：

大鼠Pcsk1重組蛋白
品名簡稱：

Recombinant Rat Pcsk1 protein
貨號：

CSB-YP017640RA
說明書：

MSDS Datasheet
規(guī)格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Pcsk1
Uniprot No.：

P28840
別名：

Pcsk1; Bdp; Nec-1; Nec1Neuroendocrine convertase 1; NEC 1; EC 3.4.21.93; Prohormone convertase 1; Proprotein convertase 1; PC1
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

73.2kDa
表達(dá)區(qū)域：

111-752aa
氨基酸序列

SVPRDSALNLFNDPMWNQQWYLQDTRMTASLPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRNVPEKKECIIKDNNFEPRALKANGEVIVEIPTRACEGQENAINSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGTWTLKVTDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVNVVEEKPTQNSLNGNLLVPKNSSSSSVEDRRDEQVQGAPSKAMLRLLQSAFSKNTPSKQSSKIPSAKLSVPYEGLYEALEKLNKPSQLEDSEDSLYSDYVDVFYNTKPYKHRDDRLLQALMDILNEKN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
基因功能參考文獻(xiàn)：
1. macrophages from PC1/3 KO mice and rat PC1/3-KD NR8383 macrophages secreted more pro-inflammatory cytokines such as TNF-alpha, IL6, IL1alpha and CXCL2. PMID: 26778167
2. PC1/3 as an important regulator of vesicle trafficking and secretion in macrophages. PMID: 23637853
3. Chronic ethanol exposure decreases prohormone convertase (pc1/3) immunoreactivity in brain. PMID: 23050949
4. catalytic and P domains contribute to the folding and thermodynamic stability of the convertases through reciprocal hydrophobic interactions (prohormone convertase 3 [pc3/pc1]) PMID: 12721373
5. The prohormone convertase (PC) enzyme PC1 together with PC2 and PC5 may represent a redundant system to ensure production of biologically active cholecystokinin in rat brain. PMID: 14608596
6. proGHRH is initially cleave by furin at preproGHRH29-30, followed by a second cleavage at preproGHRH74 primarily by PC1 to generate GHRH and GHRH-RP peptides, respectively. PMID: 15146101
7. Leptin regulates hypothalamic PC1 expression and requires prohormone processing into mature peptides. PMID: 15286802
8. Prohormone-convertase 1 processing enhances post-Golgi sorting of prothyrotropin-releasing hormone-derived peptides PMID: 16204236
9. the prohormone convertase cleavage site within the alpha-helix mediates sorting of the neuronal and endocrine polypeptide VGF into the regulated secretory pathway PMID: 16221685
10. The observed decrease in CCK seen with these RNAi treatments further supports a role for PC1 in CCK processing in these cells PMID: 16274843
11. Agouti-related protein is posttranslationally cleaved by proprotein convertase 1 to generate agouti-related protein (AGRP)83-132. PMID: 16384863
12. These data indicate that the transmembrane domain of PC3 plays a key role in sorting the enzyme to the regulated secretory pathway. PMID: 17240044
13. Ontogeny of ghrelin, obestatin, preproghrelin, and prohormone convertases in rat pancreas and stomach. PMID: 18784614
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亞細(xì)胞定位：

Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules.
蛋白家族：

Peptidase S8 family, Furin subfamily
數(shù)據(jù)庫鏈接：

KEGG: rno:25204

STRING: 10116.ENSRNOP00000015185

UniGene: Rn.11384