Recombinant Rat Myosin light chain kinase 2, skeletal/cardiac muscle (Mylk2)

中文名稱：

大鼠Mylk2重組蛋白
貨號：

CSB-YP321833RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Mylk2重組蛋白
貨號：

CSB-EP321833RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Mylk2重組蛋白
貨號：

CSB-EP321833RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Mylk2重組蛋白
貨號：

CSB-BP321833RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Mylk2重組蛋白
貨號：

CSB-MP321833RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mylk2
Uniprot No.：

P20689
別名：

Mylk2; Myosin light chain kinase 2; skeletal/cardiac muscle; MLCK2; EC 2.7.11.18
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-610
氨基酸序列

ATENGAVEL GTQSLSTDHP PTDAAGDGSP ASEKEPSLPD TEKDLGPTNT KKDPGAPDPK KNPDPPSLKK TPEAPGPEKK GDSAPASASN QGPSGEGDGG GGPAEGGTGP PAVLPQPTAT ADASIQKLDA TQAPSGNQES GEAKAGKKAA ECREAGRRGS PAFLHSPSCP AIISCSEKTL AMKPLSETTE LIFAGVSETP DPQDPGPAKD EGGTNTLADG KEEAEAGQAE QAKVQGDTSQ RIGFQAVPSE RAEVGQALCL TAKEEDCFQI LDDCPPPPAP FPHRIVELRT GNVSSEFSMN SKEALGGGKF GAVCTCTERS TGLKLAAKVI KKQTPKDKEM VLLEIEVMNQ LNHRNLIQLY SAIETSHEII LFMEYIEGGE LFERIVDEDY QLTEVDTMVF VRQICDGILF MHKMRVLHLD LKPENILCVN TTGHLVKIID FGLARRYNPN EKLKVNFGTP EFLSPEVVNY DQISDKTDMW SLGVITYMLL SGLSPFLGDD DTETLNNVLS ANWYFDEETF EAVSDEAKDF VSNLITKDQS ARMSAEQCLA HPWLNNLAEK AKRCNRRLKS QILLKKYLMK RRWKKNFIAV SAANRFKKIS SSGALMALGV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
基因功能參考文獻：
1. MLCK regulates Schwann cell cytoskeletal organization, cell differentiation and myelination. PMID: 22100921
2. Rho-kinase and MLC kinase play a role in migratory defect in FAK null cells. PMID: 12105199
3. in rat skeletal muscles an increase of MLC2 phosphorylation is associated with the slow-to-fast transition regardless of whether hypertrophy or atrophy develops. PMID: 12748068
4. role of myosin phosphorylation by myosin light chain kinase (MLCK) in regulating the invasiveness of metastatic cancer cells PMID: 12970723
5. Elk-1 regulates telokin expression PMID: 16260603
6. These data provide novel insight into the regulation of smooth muscle myosin light chain kinase (smMLCK) expression at the molecular level and demonstrate the importance of serum response factor (SRF) in regulating smMLCK promoter activity. PMID: 16822834
7. ML-7 and apocynin blocked hypoxia-dependent phosphorylation of MLC. Our data demonstrate that hypoxia causes a breakdown of the BBB in vitro and in vivo involving ROS and the contractile machinery. PMID: 17419808
8. AG repeats in the spontaneously hypertensive rats promoter increase H-DNA structures, histone modifications, and promoter activity of the smooth muscle myosin light chain kinase, perhaps contributing to vascular disorders in hypertension PMID: 17991897
9. The regulation of smMLCK expression by angiotensin II via Ras signaling is important in the regulation of vascular remodeling and blood pressure PMID: 18511912
10. PKC and MLCK pathways important in coronary endothelial dysfunction elicited by ischemia/reperfusion injury and cytokine release PMID: 18621396
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亞細胞定位：

Cytoplasm.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family
數據庫鏈接：

KEGG: rno:117558

STRING: 10116.ENSRNOP00000011255

UniGene: Rn.81191