Recombinant Rat H (+)/Cl (-) exchange transporter 3, partial

中文名稱：

Recombinant Rat H(+)/Cl(-) exchange transporter 3 ,partial
貨號：

CSB-YP345556RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat H(+)/Cl(-) exchange transporter 3 ,partial
貨號：

CSB-EP345556RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat H(+)/Cl(-) exchange transporter 3 ,partial
貨號：

CSB-EP345556RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat H(+)/Cl(-) exchange transporter 3 ,partial
貨號：

CSB-BP345556RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat H(+)/Cl(-) exchange transporter 3 ,partial
貨號：

CSB-MP345556RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Clcn3
Uniprot No.：

P51792
別名：

Clcn3H(+)/Cl(-) exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.; Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
基因功能參考文獻：
1. Galactosemia caused the osmotic stress in lenses; it also markedly leads to the upregulations of aldose reductase, P-glycoprotein, and Clcn3 in lens epithelial cells, together resulting in obvious osmotic expansion in vitro and in vivo. PMID: 29527534
2. Results have shown that the expression of ClC-3 was reduced in hypertrophic H9c2 cells, primary rat neonatal cardiomyocytes and myocardium of C57/BL/6 mice and that ClC-3 played an important role in beta-adrenergic cardiac hypertrophy. PMID: 29158167
3. Data suggest that ClC3/Clcn3 expression is up-regulated by mechanical stimulation (persistent static compression here) in osteoblastic cell line and appears to participate in mechanically sensitive osteogenesis and gene expression regulation. PMID: 26436462
4. Tyrosine 284 phosphorylation in the ClC-3 channel targeted by Src kinase is an important molecular mechanism for ClC-3 channel activation. PMID: 23536605
5. Data suggest that Clcn3 activity and chloride ion movement due to hypotonic solution are more prominent in smooth muscle cell from femoral artery than from femoral vein; regulation of Clcn3 activity via tyrosine phosphorylation is prominent in veins. PMID: 23181279
6. Our results demonstrated for the first time that rat bone marrow endothelial progenitor cells expressed volume-sensitive chloride currents and the corresponding gene and protein Clcn3. PMID: 22168445
7. ClC-3 has protective effects on H2O2-induced apoptosis of basilar artery smooth muscle cells through influencing the mitochondrial signaling pathway. PMID: 21373935
8. ClC-3 mRNA and protein expression levels were significantly higher in aortic smooth muscle and brain tissues of diabetic rats, but significantly decreased in kidney medulla tissue, relative to non-DM controls PMID: 20519092
9. Downregulation of ClC-3 channel with ClC-3 antisense transfection reduced static pressure-evoked cell proliferation and cell cycle transition. PMID: 19910686
10. ClC-3 is strongly expressed in the apical membrane of the caput epididymidis and the vas deferens and is less abundant in cells of the body and cauda epididymidis. PMID: 12475763
11. Upregulation of ClC-3 is an adaptive response of inflamed pulmonary artery, which enhances the viability of pulmonary artery smooth muscle cells against reactive oxygen species. PMID: 15723096
12. By increasing postsynaptic potentials in a Cl(-) dependent fashion, CLC-3 channels regulate neuronal excitability postsynaptically in immature neurons. PMID: 17046694
13. PAF induces intracellular acidosis via activation of the Ca(2+)-dependent Cl(-) channel ClC-3, resulting in apoptosis of intestinal epithelial cells. PMID: 18339705
14. The present study was to investigate the role of ClC-3 in cell cycle progression/distribution and the underlying mechanisms of proliferation. PMID: 18823498
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亞細胞定位：

[Isoform 1]: Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.; [Isoform 2]: Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Chloride channel (TC 2.A.49) family, ClC-3/CLCN3 subfamily
組織特異性：

Abundant in brain, especially in the olfactory bulb, hippocampus, and cerebellum. A moderate expression is seen in the lung, kidney and adrenal gland.
數據庫鏈接：

KEGG: rno:84360

STRING: 10116.ENSRNOP00000045523

UniGene: Rn.4175