Recombinant Rat Cytochrome P450 1A1 (Cyp1a1)

^{In
Stock}

中文名稱：

Recombinant Rat Cytochrome P450 1A1(Cyp1a1)
貨號：

CSB-EP006395RA
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Cyp1a1
Uniprot No.：

P00185
別名：

(CYP1A1)(CYPIA1)(Cytochrome P450 form 6)(Cytochrome P450-C)(Cytochrome P450-P1)(Cytochrome P450MT2)(Hydroperoxy icosatetraenoate dehydratase)
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

66.7 kDa
表達區域：

2-524aa
氨基酸序列

PSVYGFPAFTSATELLLAVTTFCLGFWVVRVTRTWVPKGLKSPPGPWGLPFIGHVLTLGKNPHLSLTKLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVKQGDDFKGRPDLYSFTLIANGQSMTFNPDSGPLWAARRRLAQNALKSFSIASDPTLASSCYLEEHVSKEAEYLISKFQKLMAEVGHFDPFKYLVVSVANVICAICFGRRYDHDDQELLSIVNLSNEFGEVTGSGYPADFIPILRYLPNSSLDAFKDLNKKFYSFMKKLIKEHYRTFEKGHIRDITDSLIEHCQDRRLDENANVQLSDDKVITIVFDLFGAGFDTITTAISWSLMYLVTNPRIQRKIQEELDTVIGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTIRDTSLNGFYIPKGHCVFVNQWQVNHDQELWGDPNEFRPERFLTSSGTLDKHLSEKVILFGLGKRKCIGETIGRLEVFLFLAILLQQMEFNVSPGEKVDMTPAYGLTLKHARCEHFQVQMRSSGPQHLQA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
基因功能參考文獻：
1. Assessment of the pulmonary CYP1A1 metabolism of mavoglurant (AFQ056) in rat. PMID: 28879796
2. CYP1A1 and CYP1B1 mRNA expression levels were significantly reduced from day 7 and 10, respectively. These results suggested that CpG island hypermethylation of the CYP1A1 and CYP1B1 promoters regulate the low expression of genes involved in the occurrence of isoniazidinduced liver injury PMID: 29115507
3. We showed that the enhanced activity of the CYP1A1 mutant towards TCDD was due to more efficient binding of the substrate in the active site even though the mutated site was over 2.5nm away from the catalytic center PMID: 28780123
4. Data suggest that two inhibitors from grapefruit juice (naringenin and 6',7'-dihydroxybergamottin) block oxidation of 7-ethoxyresorufin via competitive binding to the active site of both human and rat CYP1A1. PMID: 27444380
5. Hexachloronaphthalene was found to be a strong inducer of maternal and fetal CYP1A1 in liver and placenta. PMID: 26403959
6. drugs acting as D2-dopamine receptor antagonists can modify several hormone systems that regulate the expression of CYP1A1, CYP1A2 and CYP1B1, and may affect the toxicity and carcinogenicity outcome of numerous toxicants and pre-carcinogenic substances PMID: 26466350
7. Incense smoke exposure significantly induced the expression of CYP1A1, CYP1A2, and CYP1B1 mRNAs in both lung and liver tissues. PMID: 24557852
8. Differential expression of CYP1A1 and CYP1A2 genes in H4IIE rat hepatoma cells exposed to TCDD and PAHs PMID: 25555259
9. BP-lowering effect of sEH inhibition in I3C-induced Cyp1a1-Ren-2 transgenic rats. PMID: 25224811
10. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed. PMID: 25164943
11. Hypertension fails to disrupt white matter integrity in young or aged Fisher (F44) Cyp1a1Ren2 transgenic rats. PMID: 25407269
12. 5-fold reducing of vitamin content in rat diet lead to significant changes in activity and inducibility of cytochrome P450 of CYP1A and 3A family, which play a key role in the detoxification and metabolism of drugs. PMID: 25300103
13. High-altitude hypoxia decreases the activity and expression of CYP1A1 and NAT2, but increases that of CYP2D6. PMID: 24557547
14. This study aimed to assess cerebrovascular alterations in response to different durations (4 or 6 months) of controlled hypertension in an inducible transgenic rat model of hypertension (Cyp1a1-Ren2) as compared with normotensive litter mate controls. PMID: 24119481
15. Increase of CYP1A1 mRNA level may not only reduce the carcinogenicity of foreign compounds, but may also activate some cardinogens.In case of transporters, an increase of their expression in the body may lead to increased fetoprotection. PMID: 25011215
16. CYP1A1 expression in liver and ovaries is increased following chemical induction, along with microRNAs. PMID: 24727239
17. A metabolite, derived from the action of CYP1A1 and a nitroreduction-reaction process, has a key role in the bioactivation of niclosamide. PMID: 23956140
18. The results suggest that biotin does not influence the CYP1A-mediated metabolism of xenobiotics. PMID: 23984390
19. role of bilirubin, tocopherol and corticosterone as mediators of cold induction of CYP1A in the liver was evaluated; results indicate that these compounds can be involved in cold induction of CYP1A, but none of them is the only mediator in this process PMID: 23658888
20. Severe liver cirrhosis markedly reduces AhR-mediated induction of CYP1A1 in rats by decreasing the transcription of target genes. PMID: 23626760
21. The expression of genes involved in action potential generation and Ca(2+) homeostasis of cardiomyocytes in hypertensive cyp1a1ren-2 transgenic rats, was investigated. PMID: 23060473
22. CYP1A2 enzyme activity was much more markedly increased in Sprague-Dawley than Wistar rats and CYP1A1 activity was induced to similar levels. PMID: 22785257
23. In the liver, cabbage and sauerkraut juices decreased the activities of CYP1A1 and CYP1A2. PMID: 22173777
24. Organic pollutants from river water could induce the activity of the CYP1A1 gene promoter and induce the expression of CYP1A1 mRNA in H4IIE cells. PMID: 19069647
25. As(III) decreases CYP1A1, CYP1A2, CYP3A23, and CYP3A2 expression in freshly isolated rat primary hepatocytes. PMID: 22159698
26. Data from a model applying fresh smoke (or the vapor phase/particulate matter) to lung slices in 3-day culture confirm up-regulation of stress-response genes (CYP1A1, IL-1beta, and bradykinin B1 receptor) along with damage to alveolar structure. PMID: 22266391
27. Role of cytochromes P450 in metabolism of carcinogenic aristolochic acid I. PMID: 22167220
28. Molecular mechanisms of cold-induced CYP1A activation in rat liver microsomes PMID: 21505853
29. Rat Ahr and Cyp1a1 reached highest expression during gestation days 15 and 18, which might indicate different response to Ahr ligands in placental Cyp1a1 induction during rat gestation. PMID: 21666223
30. These results suggest that maternal consumption of either red raspberry leaf or some of its constituents leads to long-term alterations of CYP activity in female offspring. PMID: 21115944
31. Data show that only rLSEC-PEM-Hepatocyte cultures exhibited increasing CYP1A1/2 and CYP3A activity. PMID: 21103392
32. Amiloride reduces arterial pressure in cyp1a1ren-2 transgenic rats in association with increased renal sodium excretion without affecting renal vascular function PMID: 20683339
33. These studies demonstrated that Danshen aqueous extract affected the metabolism of CYP1A2 substrates through competitive inhibition and altered their clearance. PMID: 20663043
34. Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by recombinant yeast cells expressing either rat CYP1A1 or CYP1A2 was examined. PMID: 12054491
35. Enterocytic CYP1A (CYP1A1) showed a sharp increase at weaning, plateauing at adult levels by 60 days PMID: 12162855
36. CYP1A1 is repressed in rat keratinocytes because of the interactions of two negative regulatory elements PMID: 12200118
37. Differentiation-dependent induction of CYP1A1 in cultured rat small intestinal epithelial cells, colonocytes, and human colon carcinoma cells: basement membrane-mediated apoptosis PMID: 12210751
38. Cyp1a1 is upregulated after treatment with Aroclor in rat liver PMID: 12396271
39. analysis of metabolic pathways involving CYP1A1 and dioxins, and comparison to the human homolog PMID: 12464257
40. Hepatic upregulation of the aryl hydrocarbon receptor may modulate the potential for benzo(a)pyrene toxicity via the activation of cytochrome P450 and subsequent deposition of lipophillic metabolites to developing cerebral cortex and hippocampus. PMID: 12927582
41. Together, these results demonstrate a novel mechanism of CYP1A1 transcriptional repression that does not involve any previously reported negative regulatory elements for CYP1A1. PMID: 14592459
42. nitric oxide plays a pivotal role in the inflammation mediated loss in CYP1A activity in the brain. PMID: 15542068
43. May catalyze the oxidation of thiophene compounds with the simultaneous formation of two reactive intermediates, a thiophene-S-oxide and a thiophene epoxide. PMID: 16137656
44. Data show that maintenance of pulmonary CYP1A1 induction by iNO (20 ppm) + hyperoxia support the hypothesis that this phenomenon may contribute to the protective effects of inhaled nitric oxide against hyperoxic injury. PMID: 16492979
45. The results show that cigarette smoke (CS) and wood smoke (WS) are mutagenic but that WS differs from CS in its inability to induce pulmonary CYP1A1. PMID: 16678472
46. inhibition of benzo(a)pyrene-induced CYP1A1 activity with alpha-tocopherol is probably realized at the posttranslational level PMID: 16758613
47. effects of enteral treatment with benzo[a]pyrene on lymph node CYP 1A1/1A2 induction PMID: 16758626
48. Cancer preventive effect of soy-based diets is mediated in part by reduction in aryl hydrocarbon receptor protein level posttranslationally, reducing procarcinogen-induced CYP1A1 induction and metabolic activation. PMID: 17182795
49. results suggest that CYP1A2 plays a key role in sequence-specific 3-Methylcholanthrene-DNA adduct formation in the CYP1A1 promoter region, leading to attenuation of CYP1A1 gene expression PMID: 17276403
50. lack of NADPH oxidation in the presence of histamine showed that it is not a substrate for CYP1A1. Activity measurements using the O-dealkylation of ethoxyresorufin indicated that HA is a mixed-type inhibitor of CYP1A1 in both microsomes and supersomes. PMID: 17431589
顯示更多

收起更多
亞細胞定位：

[Cytochrome P450 1A1]: Cytoplasm.; [Cytochrome P450MT2A]: Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.; [Cytochrome P450MT2B]: Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
蛋白家族：

Cytochrome P450 family
組織特異性：

Liver.
數據庫鏈接：

KEGG: rno:24296

STRING: 10116.ENSRNOP00000026473

UniGene: Rn.10352