Recombinant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylase (pobA)

中文名稱：

銅綠假單胞菌pobA重組蛋白
貨號：

CSB-YP324066EZX
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

銅綠假單胞菌pobA重組蛋白
貨號：

CSB-EP324066EZX
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

銅綠假單胞菌pobA重組蛋白
貨號：

CSB-EP324066EZX-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

銅綠假單胞菌pobA重組蛋白
貨號：

CSB-BP324066EZX
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

銅綠假單胞菌pobA重組蛋白
貨號：

CSB-MP324066EZX
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pobA
Uniprot No.：

P20586
別名：

pobA; PA0247; p-hydroxybenzoate hydroxylase; PHBH; EC 1.14.13.2; 4-hydroxybenzoate 3-monooxygenase
種屬：

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
蛋白長度：

full length protein
表達區域：

1-394
氨基酸序列

MKTQVAIIGA GPSGLLLGQL LHKAGIDNVI LERQTPDYVL GRIRAGVLEQ GMVDLLREAG VDRRMARDGL VHEGVEIAFA GQRRRIDLKR LSGGKTVTVY GQTEVTRDLM EAREACGATT VYQAAEVRLH DLQGERPYVT FERDGERLRL DCDYIAGCDG FHGISRQSIP AERLKVFERV YPFGWLGLLA DTPPVSHELI YANHPRGFAL CSQRSATRSR YYVQVPLSEK VEDWSDERFW TELKARLPSE VAEKLVTGPS LEKSIAPLRS FVVEPMQHGR LFLAGDAAHI VPPTGAKGLN LAASDVSTLY RLLLKAYREG RGELLERYSA ICLRRIWKAE RFSWWMTSVL HRFPDTDAFS QRIQQTELEY YLGSEAGLAT IAENYVGLPY EEIE
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the incorporation of an atom of dioxygen into p-hydroxybenzoate (p-OHB) to form 3,4-dihydroxybenzoate (3,4DOHB). The reaction occurs in two parts: reduction of the flavin adenine dinucleotide (FAD) in the enzyme by reduced nicotinamide adenine dinucleotide phosphate (NADPH) in response to binding p-hydroxybenzoate to the enzyme and oxidation of reduced FAD with oxygen to form a hydroperoxide, which then oxygenates p-hydroxybenzoate.
基因功能參考文獻：
1. The hydrogen-bond network in p-hydroxybenzoate hydroxylase works to enhance both the hydroxylation reaction and the process that completes the catalytic cycle. PMID: 15568817
2. The effect of the Ala45Gly mutation on the structure and function of p-hydroxybenzoate hydroxylase (PHBH) illustrates the importance of the si side loop in the conformational state of PHBH and, consequently, the function of the enzyme. PMID: 15924424
3. kinetic analysis of proton-linked flavin conformational changes in p-hydroxybenzoate hydroxylase PMID: 16201756
蛋白家族：

Aromatic-ring hydroxylase family
數據庫鏈接：

KEGG: pae:PA0247

STRING: 208964.PA0247