Recombinant Mouse T-cell surface glycoprotein CD3 epsilon chain (Cd3e), partial

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中文名稱：

Recombinant Mouse T-cell surface glycoprotein CD3 epsilon chain(Cd3e),partial
貨號(hào)：

CSB-YP004931MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004931MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cd3e.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004931MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cd3e.
其他：

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產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Cd3e
Uniprot No.：

P22646
別名：

Cd3eT-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Extracellular Domain
來(lái)源：

Yeast
分子量：

11.9 kDa
表達(dá)區(qū)域：

23-108aa
氨基酸序列

DAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDFSEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

Recombinant Mouse T-cell surface glycoprotein CD3 epsilon chain (Cd3e)是經(jīng)過(guò)重組制備的小鼠T細(xì)胞表面糖蛋白CD3 epsilon鏈。CD3 epsilon鏈?zhǔn)荰細(xì)胞受體（TCR）復(fù)合物的重要組成部分，負(fù)責(zé)傳遞外界抗原刺激并激活T細(xì)胞。CD3 epsilon鏈通過(guò)與其他CD3亞單位和TCR亞單位相互作用，形成一個(gè)穩(wěn)定的T細(xì)胞表面信號(hào)傳導(dǎo)復(fù)合物。這個(gè)復(fù)合物的形成是T細(xì)胞活化和免疫應(yīng)答的關(guān)鍵步驟。

華美生物重組Cd3e蛋白由酵母表達(dá)系統(tǒng)制備，具有較高的純度和穩(wěn)定性，可用于分子生物學(xué)、免疫學(xué)和免疫治療研究中。通過(guò)研究Cd3e蛋白的結(jié)構(gòu)和功能，科學(xué)家們可以更好地了解TCR復(fù)合物的組裝和信號(hào)傳導(dǎo)機(jī)制，揭示T細(xì)胞的激活和調(diào)節(jié)過(guò)程。Cd3e的研究不僅對(duì)于基礎(chǔ)的免疫學(xué)理解具有重要意義，還在免疫治療領(lǐng)域具有潛在應(yīng)用價(jià)值。通過(guò)調(diào)控Cd3e蛋白的功能，可以影響T細(xì)胞的活化和功能，從而干預(yù)免疫應(yīng)答、治療免疫相關(guān)的疾病。
Datasheet & COA：

Please contact us to get it.

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■ 常見(jiàn)問(wèn)題解答

We are interested in item CSB-YP004931MO. For the current lot, would you please tell me the buffer before the product was lyophilized? What do you recommend for reconstitution?
Would you have this information available to share?

Thanks for your email.
The stock of this protein has just run out. The new lot is already under production and we will rush the preparation.
1. This protein has been delivered in liquid form in the following two buffers before: 1) PBS, pH 7.4, 50% glycerol; 2) 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerol.
2. If you would like Lyophilized Powder, we will add 6% trehalose to help dissolving later. The lead time will be extended for 3 working days. You can reconstitute the lyophilized protein directly with sterile deionized water. The general reconstitution method is as follows
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. You could use it as reference.
Any more questions, feel free to contact us. Nice day.

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靶點(diǎn)詳情

功能：

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
基因功能參考文獻(xiàn)：
1. The data show that CD3epsilon chain couples to Cbl via simultaneous binding of both proteins to Numb, thus mediating TCR degradation PMID: 26507128
2. IL-17-producing CD3(bright) gammadelta T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. PMID: 25385067
3. these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling. PMID: 24638062
4. these results indicate that membrane association of the CD3epsilon signaling domain is required for optimal thymocyte development and peripheral T cell function. PMID: 24899501
5. These results suggest that proteasome-mediated degradation is involved in hypophosphorylated LAT and PLCgamma1 in Dow2-induced anergic T cells. The novel CD3-specific Ab, Dow2, may provide us with a unique tool for inducing immunosuppression PMID: 24595757
6. that Nck recruitment to the TCR is fundamental to mount an efficient T cell response in vivo, and that the Nck-CD3epsilon interaction may represent a target for pharmacological modulation of the immune response. PMID: 24470497
7. Structure of murine CD3epsilon complexed with the mitogenic anti-CD3epsilon antibody 2C11 enabled structural comparisons of antibody-liganded and unliganded forms of CD3epsilon revealing that antibody binding does not induce any substantial rearrangements within CD3epsilon. PMID: 22262845
8. CFTR dysfunction in T cells can lead directly to aberrant immune responses in CD3+ lymphocytes PMID: 20724552
9. analysis of the transgenic integration site in immunodeficient tgepsilon26 human CD3epsilon transgenic mice PMID: 21203507
10. Polymorphisms of the CD3varepsilon ectodomain exist in mice,some of which lead to amino acid substitutions which cause structural changes and affect anti-CD3 antibody binding. PMID: 20638133
11. the stalks of the CD3 proteins may be critical in transmitting part of the activation signal directly through the membrane. PMID: 19956738
12. Results suggest that generation of CD3varepsilon chain isoforms with different N-terminal sequence and pI is a general phenomenon. PMID: 19616027
13. We demonstrate that the intra-cytoplasmic (IC) domain of CD3epsilon plays a critical role in regulating TCR expression on DP thymocytes. PMID: 19819936
14. inability of Nck to bind to the CD3epsilon proline-rich sequence in thymocytes after TCR ligation PMID: 15972658
15. That CD43 costimulation was responsible for elevated cytokine/chemokine activity was confirmed at the transcriptional level by real-time PCR for IFN-gamma and CCL5, and by ELISA for IFN-gamma. PMID: 16246302
16. insulin-specific Tregs producing IL-10, TGF-beta, and IL-4 are enhanced by suppression of CD3epsilon in a mouse model of autoimmune diabetes PMID: 16628253
17. GRK2 is primarily involved in arresting G protein-coupled receptor signals, its interaction with CD3 epsilon may provide a novel means whereby the TCR can negatively regulate signals generated through G protein-coupled receptors. PMID: 17420248
18. FcgammaRIIB, a low-affinity immunoglobulin G Fc receptor, and CD3 are involved in cerebellar functions. PMID: 17502348
19. CD3epsilon-mediated signal transduction pathway is essential for this transformation process PMID: 17507663
20. mouse CD3 epsilon chains N-terminal charged residues have roles in generating isoforms modulating antigen T cell receptor-mediated signals and T cell receptor-CD3 interactions PMID: 17561508
21. CD3epsilon proline-rich sequence amplifies weak TCR signals by promoting synapse formation and CD3epsilon phosphorylation PMID: 18566390
22. have identified distinct roles for individual motifs of CD3epsilon in the preTCR-mediated differentiation and proliferation PMID: 19342663
23. functional role for the CD3 epsilon lipid-binding domain in T cell biology PMID: 19542373
24. importance of the conformational change in CD3epsilon for the activation of T cells PMID: 19671929
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亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:12501

STRING: 10090.ENSMUSP00000099896

UniGene: Mm.210361