Recombinant Mouse Vasodilator-stimulated phosphoprotein (Vasp)

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中文名稱：

小鼠Vasp重組蛋白
貨號：

CSB-EP025797MO
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Vasp
Uniprot No.：

P70460
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

45.5 kDa
表達(dá)區(qū)域：

2-375aa
氨基酸序列

SETVICSSRATVMLYDDSNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIIRGVKYNQATPIFHQWRDARQVWGLNFGSKEDAIQFATGMANALEALEGGGPPPAPAPPAWSAQNGPSPEELEQQKRQPEHMERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPSSGVSGAGHGAGAAPPPAPPLPTAQGPNSGGSGAPGLAAAIAGAKLRKVSKQEEASGGPLAPKAENSRSTGGGLMEEMNAMLARRRKATQVGEKPPKDESASEESEARLPAQSEPVRRPWEKNSTTLPRMKSSSSVTTSEAHPSTPCSSDDSDLERVKQELLEEVRKELQKMKEEIIEVFVQELRKRGSP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
基因功能參考文獻(xiàn)：
1. Myocardial ischemic preconditioning-induced VASP phosphorylation in platelets is a protective mechanism against the deleterious effects of ischemia. PMID: 29344719
2. VASP selectively mediate activated T-cell trafficking by promoting the diapedesis step of transendothelial migration in a alpha4 integrin-dependent manner. PMID: 28320969
3. We identified a phosphorylation-dependent mechanism that regulates selective recruitment of these effectors to Lamellipodin: Abl-mediated Lamellipodin phosphorylation promotes its association with both Scar/WAVE and Ena/VASP, whereas Src-dependent phosphorylation enhances binding to Scar/WAVE but not to Ena/VASP PMID: 26996666
4. Thus, unlike host proteins characterized in Shigella pathogenesis that promote bacterial spread, VASP and EVL function to limit it. PMID: 26358985
5. Data show that the hypoxia inducible factor 1 alpha subunit (HIF-1alpha) protein level in lung tissues increased significantly at four hours and eight hours, whereas the vasodilator-stimulated phosphoprotein (VASP) protein level decreased significantly. PMID: 25051011
6. Collectively, our studies highlighted that the CuB-induced actin aggregation and cofilin-actin rod formation was mediated via the Ga13/RhoA/PKA/VASP pathway. PMID: 24691407
7. Ena/VASP regulates mDia2-initiated filopodial morphology, dynamics, and function. PMID: 24989797
8. cancer cells reaching liver sinusoids induced up-regulation of VASP PMID: 24917558
9. Low VASP activation was associated with high fat diet (HFD). Effects of HFD on aortic inflammation and insulin resistance were recapitulated by VASP knockout, implying a role for VASP to constrain inflammatory signaling and maintain insulin sensitivity. PMID: 25117404
10. CDC42 activation inhibits this activity and promotes IRSp53-dependent recruitment and clustering of VASP to drive actin assembly. PMID: 24076653
11. VASP physically interacted with IRSp53 in NIH-Src cells and was essential for podosome formation. PMID: 23555988
12. A VASP to Rac to soluble guanylyl cyclase negative feedback loop limited cGMP production, thereby regulating adipogenesis and energy homeostasis. PMID: 22932701
13. The results of this study suggested that that PI(3,4)P2, Lpd, and Ena/VASP are involved in the process movement of multipolar migrating cells. PMID: 22915108
14. study demonstrate that endothelial VASP holds significant importance for endothelial barrier properties during hypoxia PMID: 21607702
15. studies identified VASP and VASP phosphorylation as crucial target for future hepatoprotective strategies PMID: 22216296
16. Data indicate that VASP is a critical downstream mediator of the anti-inflammatory effects induced by the NO/cGMP pathway. Targeted deletion of VASP predisposes to Kupffer cell inflammation. PMID: 21911751
17. phosphorylation of VASP by AMPK occurs at a novel site, serine 322, and phosphorylation at this site alters actin filament binding. PMID: 21945940
18. VASP binding to actin, elevated Rac activity, and elevated formation of actin free barbed ends, thus restoring normal beta(2) integrin function. PMID: 21795685
19. Growth of VASP expressing melanomas was retarded in VASP(-/-) versus wild-type animals PMID: 21762694
20. The phosphorylation of VASP performs a key function for the formation of platelet-neutrophil complexes that is crucially important for the extent of myocardial ischemia-reperfusion injury. PMID: 21606399
21. VASP is the critical regulator of BBB maintenance during acute ischemic stroke PMID: 21151938
22. VASP deficiency leads to a more profound endothelial barrier disruption and delayed recovery after activation of thrombin PAR-1 receptor. PMID: 20945373
23. A link is proposed between vimentin, VASP phosphorylation and actin dynamics that delivers an explanation for the important role of vimentin in controlling endothelial cell morphogenesis. PMID: 20382123
24. Data demonstrate a novel mechanism by which alphavbeta3 integrin acts to locally suppress beta1 integrin activation and regulate VASP and RIAM to control cell adhesion and migration. PMID: 20404115
25. VASP-deficient platelets display normal filopodia extension PMID: 19806269
26. Results suggest that VASP phosphorylation by PKA plays an important role in membrane ruffle formation and chemotaxis via the regulation of focal adhesion formation/maturation. PMID: 19733667
27. IRAK-1 forms a close complex with PKCepsilon as well as VASP, and participates in phorbol 12-myristate 13-acetate-induced phosphorylation of VASP. PMID: 20044140
28. VASP phosphorylation controls remodeling of the actin cytoskeleton. PMID: 19825941
29. These results identify tissue-specific VASP as a central protein in the control of the alveolar-capillary barrier properties during acute lung injury. PMID: 19690214
30. Results reveal a vasodilator-stimulated phosphoprotein (VASP)-dependent modulation of the Rac/PAK pathway and Rac/PAK-regulated processes, like cell motility and polarization. PMID: 12055190
31. function as a cellular regulator of actin dynamics perhaps via initializing actin polymerization PMID: 12372613
32. Ena-VASP proteins may play an important role in intercalated disk function at the interface between cardiac myocytes. PMID: 12933343
33. VASP is involved in down-regulation of platelet adhesion to the vascular wall under both physiologic and pathophysiologic conditions. PMID: 12933589
34. findings indicate that Ena/VASP proteins contribute to the persistence of both speed and directionality of L. monocytogenes movement PMID: 12940993
35. Mena & VASP are needed for viability & the formation of neural-derived organs. Neural tube, craniofacial, spinal nerve, & anterior commissure defects are seen in double but not single M-/- & V-/- mutants, showing both are needed for the above structures. PMID: 15371503
36. Despite an enhanced injury response early on, VASP -/- mice are protected from long-term progression of nephrotoxic nephritis, which is associated with improved renal endothelial cell preservation and regeneration. PMID: 15743999
37. delayed hearing development in VASP-/- mice is supposed to be caused by a delayed formation of actin filaments in the outer pillar head plate indicating the importance of appropriate pillar cell stiffness in cochlear mechanics PMID: 17361081
38. Both recruitment of SH2-Bbeta to Listeria and SH2-Bbeta stimulation of actin-based propulsion require the vasodilator-stimulated phosphoprotein (VASP), which binds ActA at the surfaces of Listeria cells and enhances bacterial actin-based motility. PMID: 17452473
39. VASP has a role during neonatal development of the mammalian cochlea PMID: 17920567
40. Loss of murine Ena/VASP protein, an actin regulatory protein, causes neuronal ectopias, alters intralayer positioning in the cortical plate, and, surprisingly, blocks axon fiber tract formation during corticogenesis. PMID: 17988629
41. VASP at least in part stabilizes endothelial barrier functions by regulating Rac1 activity. PMID: 17989211
42. VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells. PMID: 19118163
43. Data indicate that VASP is required for integrin alpha5beta1-mediated adhesion which stabilizes endothelial barrier properties at least in part by facilitating Rac 1 activation. PMID: 19347869
44. Regulation of VASP phosphorylation in cardiac myocytes: differential regulation by cyclic nucleotides and modulation of protein expression in diabetic and hypertrophic heart. PMID: 19734360
45. VASP phosphorylation at serine239 regulates cytoskeleton remodeling. PMID: 19798690
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亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane.
蛋白家族：

Ena/VASP family
組織特異性：

Highly expressed in thymus and spleen. Lower levels in lung, ovary, placenta and fat.
數(shù)據(jù)庫鏈接：

KEGG: mmu:22323

STRING: 10090.ENSMUSP00000032561

UniGene: Mm.9684