Recombinant Mouse Unconventional myosin-VIIa (Myo7a), partial

中文名稱：

小鼠Myo7a重組蛋白
貨號(hào)：

CSB-YP015352MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Myo7a重組蛋白
貨號(hào)：

CSB-EP015352MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Myo7a重組蛋白
貨號(hào)：

CSB-EP015352MO-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Myo7a重組蛋白
貨號(hào)：

CSB-BP015352MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Myo7a重組蛋白
貨號(hào)：

CSB-MP015352MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Myo7a
Uniprot No.：

P97479
別名：

Myo7a; Myo7; Unconventional myosin-VIIa
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.
基因功能參考文獻(xiàn)：
1. Many of the genes found in the genetic network, pathways, and gene ontology categories of Myo7a are related to either deafness or blindness in Usher syndrome mouse model. PMID: 29430167
2. Here, the authors show that exophilin-8 accumulates granules in the cortical F-actin network not by direct interaction with myosin-Va, but by indirect interaction with a specific form of myosin-VIIa through its previously unknown binding partner, RIM-BP2. PMID: 28673385
3. MYO7A and PDZD7 interact in tissue-culture cells, and co-localize to the ankle-link region of stereocilia in wild-type but not Myo7a mutant mice. PMID: 27525485
4. MYO7A binds to and impinges on CASPASE-8, revealing a new regulatory axis affecting RIPK1>CASPASE-8 signaling. Results expose a conserved role for unconventional myosins in transducing caspase-dependent regulation of kinases. PMID: 26960254
5. Data show that myosin7a (Myo7a; sh1) deficiency causes severe retinal dysfunctions in albino sh1-/- mice. PMID: 23991031
6. The family was found to segregate novel mutations of two different genes: myosin VIIA (MYO7A), and phosphodiesterase 6B, which causes nonsyndromic retinitis pigmentosa. PMID: 23882135
7. the importance of MYO7A for the development and maintenance of bundle function PMID: 22381527
8. the results support a role for MYO7A in the translocation of RPE65, illustrating the involvement of a molecular motor in the spatiotemporal organization of the retinoid cycle in vision. PMID: 21493626
9. the myosin VIIa is a "slow", monomeric molecular motor with a duty ratio of 0.6. PMID: 21212272
10. crystal structure of MYO7A MyTH4-FERM domains in complex with the central domain (CEN) of Sans at 2.8 angstrom resolution; MyTH4-FERM/CEN complex structure provides mechanistic explanations for known deafness-causing mutations in MYO7A MyTH4-FERM PMID: 21311020
11. Cadherin-23, myosin VIIa and harmonin form a ternary complex and interact with phospholipids. PMID: 20639393
12. MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear PMID: 19774077
13. The carboxy-terminal FERM domain of myosin VIIA is critical for melanosome transport in retinal pigment epithelial cells. PMID: 20016096
14. participates in anchoring and holding membrane-bound elements to the actin core of the stereocilium PMID: 11753415
15. Cdh23 and Myo7a are both required for establishing and/or maintaining the proper organisation of the stereocilia bundle and that they do not genetically interact to affect this process nor to cause age-related hearing loss. PMID: 12121736
16. Slac2-c interacts with Rab27, actin, myosin Va and this protein PMID: 12221080
17. the shaping of the hair bundle relies on a functional unit composed of myosin VIIa, harmonin b and cadherin 23 that is essential to ensure the cohesion of the stereocilia PMID: 12485990
18. Noise-induced hearing loss in 11-12-week-old mice heterozygous for a null allele of Myo7a ( Myo7a(4626SB)) is not significantly different from wild-type littermates. PMID: 14648237
19. A Myo7a mutation cosegregates with stereocilia defects and low-frequency hearing impairment. PMID: 15389316
20. Slac2-c acts as a functional myosin VIIa receptor and the Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates the transport of retinal melanosomes in pigment epithelium cells PMID: 15927964
21. Protocadherin 15 and myosin 7a cooperate to regulate the development and function of the mechanically sensitive hair bundle. PMID: 16481439
22. The present results provide evidence from live retinal pigment epithelium cells that the RAB27A-MYRIP-MYO7A complex functions in melanosome motility. PMID: 17352418
23. Shroom2 and ZO-1 form a tight-junction-associated scaffolding complex, possibly linked to myosin VIIa, that bridges the junctional membrane to the underlying cytoskeleton, thereby contributing to the stabilization of these junctions. PMID: 17666436
24. Mosaic complementation demonstrates a regulatory role for Myo7a in actin dynamics of stereocilia. PMID: 18160714
25. The data indicate that melanosomes in the retinal pigment epithelium and choroid are the dominant source of NIR-autofluorescence from the posterior region of the eye with ushers syndrome due to MYO7A mutation. PMID: 19324852
26. A MYO7A-MYRIP-RAB27A complex is needed for apical melanosome tethering and regulates transport in RPE (retinal pigment epithelium) cells. MYRIP recruitment to the apical RPE is independent of RAB27A or melanosomes. PMID: 17352418
27. Functional replacement with human MYO7A using lentiviral vectors rescues defects in opsin transport, melanosome transport and degradation of rod outer segment membranes in the retina's of shaker1 mice null for Myo7a PMID: 17268537
28. myosin VIIa is required for the correct transport and degradation of ingested photoreceptor outer segment membranes by the RPE (retinal pigmented epithelium) PMID: 12743369
29. myosin VIIa and Rab27a regulate the transport and tethering of RPE (retinal pigmented epithelium) melanosomes PMID: 15572405
30. Shown to be a functional mechanoenzyme with actin activated ATPase activity as well as being a calcium sensitive calmodulin binding protein. PMID: 11839794
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相關(guān)疾病：

Defects in Myo7a are the cause of the shaker-1 (sh-1) phenotype which affects only the inner ear. Sh-1 homozygote mutants show hyperactivity, head tossing and circling due to vestibular dysfunction, together with typical neuroepithelial-type cochlear defects involving dysfunction and progressive degeneration of the organ of Corti.
亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell junction, synapse.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
組織特異性：

Detected in mechanosensory stereocilia of cochlea hair cells (at protein level). Expressed in the retina, cochlea, kidney and liver.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:17921

STRING: 10090.ENSMUSP00000102745

UniGene: Mm.1403