Recombinant Mouse Transcriptional enhancer factor TEF-3 (Tead4), partial

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中文名稱(chēng)：

小鼠Tead4重組蛋白
品名簡(jiǎn)稱(chēng)：

Recombinant Mouse Tead4 protein, partial
貨號(hào)：

CSB-EP737076MO2b1
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Tead4
Uniprot No.：

Q62296
別名：

Tead4; Tcf13r1; Tef3; Tefr1; Transcriptional enhancer factor TEF-3; ETF-related factor 2; ETFR-2; TEA domain family member 4; TEAD-4; TEF-1-related factor 1; TEF-1-related factor FR-19; RTEF-1
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
來(lái)源：

E.coli
分子量：

33.0 kDa
表達(dá)區(qū)域：

210-427aa
氨基酸序列

RSIASSKLWMLEFSAFLERQQDPDTYNKHLFVHISQSSPSYSDPYLETVDIRQIYDKFPEKKGGLKELFERGPSNAFFLVKFWADLNTNIDDEGSAFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYLYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. Might play a role in the embryonic development of skeletal muscle.
基因功能參考文獻(xiàn)：
1. TEAD4 mediated the interaction between YAP1 and c-JUN. YAP1 and the YAP1-TEADs complex have roles in regulating osteoclastogenesis and related gene expression. PMID: 29432919
2. Based on the similarity to a related family member, and in vitro transcription/translation product(s), the authors propose that translation initiates from a non-AUG (AUA or AUU) start codon. PMID: 8920920
3. AP-1- and TEAD4-associated cis-regulatory elements form hubs for multiple signalling-responsive transcription factors and define the cistrome that regulates vascular and hematopoietic development by extrinsic signals. PMID: 27802171
4. Dual-luciferase reporter gene analysis showed that RTEF-1 is a direct target of mir-125a-5p, which regulates angiogenesis by repressing RTEF-1 expression and modulating eNOS and VEGF expression. PMID: 25059272
5. TEAD4 establishes the energy homeostasis essential for blastocoel formation. PMID: 23903192
6. These results show that RTEF-1-stimulated IGFBP-1 expression may be central to the mechanism by which RTEF-1 attenuates blood glucose levels. PMID: 22843786
7. Vgll1 interacts with TEAD4 in a manner similar to the transcription coactivators, as well as oncogenes YAP and TAZ, despite having a varied primary sequence. Vgll1 has the potential to promote cancer progression. PMID: 22632831
8. endothelial-specific RTEF-1 overexpressing mice had enhanced angiogenic sprouting and vascular structure remodeling, resulting in the formation of a denser and more highly interconnected superficial capillary plexus PMID: 22652601
9. Data suggest that altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specification. PMID: 22529382
10. TEAD factors directly induce Myogenin, CDKN1A and Caveolin 3 expression to promote myoblast differentiation. PMID: 21701496
11. Gata3 and Cdx2 can act in parallel pathways downstream of Tead4 to induce the expression of common and independent targets in the trophoblast lineage, whereas Oct4 is required for continued repression of trophoblast fate in the embryonic lineage PMID: 20081188
12. Novel RTEF-1 transcripts are present within human ocular vascular endothelial cells and mouse neural retina during normal and retinopathy of prematurity development, and alternatively spliced products are produced under hyperoxic and hypoxic conditions PMID: 17652751
13. RTEF-1 regulated smooth muscle alpha-actin transcription in myofibroblasts, but not in differentiated smooth muscle PMID: 17823374
14. Tead4 is the earliest gene shown to be uniquely required for specification of the trophectoderm lineage PMID: 17913785
15. Tead4 has a distinct role from Tead1 and Tead2 in trophectoderm specification of pre-implantation embryos; it is an early transcription factor required for specification and development of the trophectoderm lineage, which includes expression of Cdx2. PMID: 18083014
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亞細(xì)胞定位：

Nucleus.
組織特異性：

Preferentially expressed in lung and in skeletal muscle.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:21679

STRING: 10090.ENSMUSP00000006311

UniGene: Mm.14774