Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase (Cyp17a1)

中文名稱：

Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase(Cyp17a1)
貨號：

CSB-YP006392MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase(Cyp17a1)
貨號：

CSB-EP006392MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase(Cyp17a1)
貨號：

CSB-EP006392MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase(Cyp17a1)
貨號：

CSB-BP006392MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Steroid 17-alpha-hydroxylase/17,20 lyase(Cyp17a1)
貨號：

CSB-MP006392MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Cyp17a1
Uniprot No.：

P27786
別名：

Cyp17a1; Cyp17Steroid 17-alpha-hydroxylase/17,20 lyase; EC 1.14.14.19; 17-alpha-hydroxyprogesterone aldolase; EC 1.14.14.32; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區域：

1-507
氨基酸序列

MWELVGLLLL ILAYFFWPKS KTPNAKFPRS LPFLPLVGSL PFLPRRGHMH ANFFKLQEKY GPIYSLRLGT TTAVIVGHYQ LAREVLVKKG KEFSGRPQMV TLGLLSDQGK GVAFADSSSS WQLHRKLVFS TFSLFRDDQK LEKMICQEAN SLCDLILTYD GESRDLSTLI FKSVINIICT ICFNISFENK DPILTTIQTF TEGIVDVLGH SDLVDIFPWL KIFPNKNLEM IKEHTKIREK TLVEMFEKCK EKFNSESLSS LTDILIQAKM NAENNNTGEG QDPSVFSDKH ILVTVGDIFG AGIETTSSVL NWILAFLVHN PEVKRKIQKE IDQYVGFSRT PSFNDRTHLL MLEATIREVL RIRPVAPLLI PHKANIDSSI GEFAIPKDTH VIINLWALHH DKNEWDQPDR FMPERFLDPT GSHLITPTPS YLPFGAGPRS CIGEALARQE LFIFMALLLQ RFDFDVSDDK QLPCLVGDPK VVFLIDPFKV KITVRQAWKD AQVEVST
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
基因功能參考文獻：
1. through mouse-to-human search and validation, we found that CYP17A1 is overexpressed in hepatocellular carcinomas and it has great potentiality as a noninvasive marker for hepatocellular carcinoma detection PMID: 27339169
2. Demonstration of the physiologic importance of cytochrome b5 activating the 17,20-lyase reaction and the production of 19-carbon sex steroids from 21-carbon precursors in mice. PMID: 26974035
3. TRalpha/T3 directly acts on the P450c17 promoter, which contains putative thyroid response elements, and indirectly acts on the promoter of steroidogenic enzyme genes by modulating Nur77 transactivation on these promoters. PMID: 24725081
4. ata indicate that androgen receptor (AR) regulation on Klk27, Rhox5, Eppin, and Cyp17 genes in adult mouse testis using chromatin immunoprecipitation (ChIP) assays. PMID: 23838579
5. Data suggest that newly synthesized protein(s) are required for cAMP induction of CYP17 and 3-beta-HSD mRNA levels (but not of CYP11A mRNA levels). [REVIEW] PMID: 22217834
6. Data suggest that immune-activation of testicular interstitial macrophages may cause inhibition of P450c17 gene expression in Leydig cells. PMID: 22217835
7. Mamld1 enhances Cyp17a1 expression primarily in Leydig cells and permit to produce a sufficient amount of testosterone for male sex development, independently of the Hes3-related non-canonical Notch signaling PMID: 21559465
8. Fxr-/-Shp-/- mice exhibited cholestasis and liver injury as early as 3 weeks of age with strong induction of cytochrome P450, family 17, subfamily a, polypeptide 1 (Cyp17a1) PMID: 21123943
9. cAMP induces expression of Cyp17 by a PKA-mediated mechanism which is inhibited by MIS signal transduction PMID: 12193547
10. CYP17, in addition to its 17alpha-hydroxylase/17,20-lyase activity, critical in androgen formation, also expresses a secondary activity, squalene monooxygenase (epoxidase). PMID: 15761033
11. CYP17 deletion causes infertility in male. PMID: 15890676
12. These results implicate Src tyrosine kinase in the regulation of CYP17 and thecal androgen secretion. PMID: 18642057
13. P450c17 is expressed in mouse embryonic stem cells, its expression increases upon differentiation to an early embryonic endoderm lineage. PMID: 18832096
14. Results suggest that in the adult mouse brain, dehydroepiandrosterone is formed via a Fe(2+)-sensitive cytochrome P450 17alpha-hydroxylase/17, 20 lyase-independent pathway. PMID: 19500726
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亞細胞定位：

Endoplasmic reticulum membrane. Microsome membrane.
蛋白家族：

Cytochrome P450 family
數據庫鏈接：

KEGG: mmu:13074

STRING: 10090.ENSMUSP00000026012

UniGene: Mm.1262