Recombinant Mouse Serine protease inhibitor Kazal-type 1 (Spink1)

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Stock}

中文名稱：

小鼠Spink1重組蛋白
貨號：

CSB-YP362579MO
說明書：

MSDS Datasheet
規格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Spink1
Uniprot No.：

P09036
別名：

Spink1; Spink3; Serine protease inhibitor Kazal-type 1; P12; Prostatic secretory glycoprotein; Serine protease inhibitor Kazal-type 3
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

8.1kDa
表達區域：

24-80aa
氨基酸序列

AKVTGKEASCHDAVAGCPRIYDPVCGTDGITYANECVLCFENRKRIEPVLIRKGGPC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The recombinant mouse Spink1 protein is a fusion protein consists of the mouse Spink1 protein (24-80aa) partnered with the N-terminal 6xHis tag. It was produced in the yeast. This recombinant Spink1 protein's purity is greater than 90% determined by SDS-PAGE. After electrophoresis, there is a 12 kDa protein band presented on the gel.
SPINK1 is a secreted protein known as a protease inhibitor of trypsin in the pancreas. However, emerging evidence shows its function in promoting cancer progression in various types of cancer. It was first discovered in bovine pancreas extracts. SPINK1 is also known as tumor-associated trypsin inhibitor (TATI), was further isolated from the urine of ovarian cancer patients by another research group. SPINK1 and TATI were later characterized as identical molecules. The human SPINK1 gene encodes an mRNA that is spliced into three transcript variants, which can be translated into a 79-amino acid peptide, including a 23-amino-acid signal peptide. Interestingly, a SPINK1 interaction with epidermal growth factor receptor (EGFR) in rat liver hepatocyte line of BRL-3A in vitro has been indicated. SPINK1 reprograms the expression profile of prostate cancer cells, leading to prominent epithelial–endothelial transition (EET), a phenotypic switch mediated by EGFR signaling. Presently, the interaction of SPINK1 has attracted attention for its potentially pivotal biological functions, especially in modulating cancer progression.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.; In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
基因功能參考文獻：
1. It is a trypsin-specific inhibitor in the pancreas. PMID: 24521862
2. IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3 PMID: 22042864
3. SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity. PMID: 22228629
4. uterine-gland-derived SPINK3, as a new paracrine modulator, might play an important role in embryo implantation through its influence on stromal decidualization in mice. PMID: 20623140
5. Spink3 may act as a growth factor, leading to cell proliferation and regeneration of tissue structure. PMID: 20660988
6. Results suggest that regulation of Prss1 and Spink3 expression is involved in the susceptibility to experimentally induced pancreatitis. PMID: 20157294
7. Spink3 trypsin inhibitor is required for normal pancreatic development. Data indicate an important relationship between endogenous pancreatic trypsin and Spink3 and the development of pancreatitis. PMID: 20110462
8. SPINK3 mRNA is upregulated in chronic pancreatitis. PMID: 15097871
9. Spink3 may play important roles in proliferation and/or differentiation of various cell types during development. PMID: 18386042
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亞細胞定位：

Secreted.
組織特異性：

In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.
數據庫鏈接：

KEGG: mmu:20730

STRING: 10090.ENSMUSP00000025381

UniGene: Mm.272