Recombinant Mouse Retinal dehydrogenase 2 (Aldh1a2)

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中文名稱：

Recombinant Mouse Retinal dehydrogenase 2(Aldh1a2)
貨號(hào)：

CSB-EP720287MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Aldh1a2
Uniprot No.：

Q62148
別名：

(RALDH 2)(RalDH2)(Aldehyde dehydrogenase family 1 member A2)(Retinaldehyde-specific dehydrogenase type 2)(RALDH(II)
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

E.coli
分子量：

62.6 kDa
表達(dá)區(qū)域：

1-518aa
氨基酸序列

MTSSEIAMPGEVKADPAALMASLQLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVCNPATGEQVCEVQEADKVDIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRATLATMESLNGGKPFLQAFYIDLQGVIKTLRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFTWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEAGFPPGVVNILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVKRSVERAKRRIVGSPFDPTTEQGPQIDKKQYNKVLELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALMVSSAMQAGTVWINCYNALNAQSPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Converts retinaldehyde to retinoic acid. Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Lacks activity with benzaldehyde, acetaldehyde and octanal. Displays complete lack of activity with citral.
基因功能參考文獻(xiàn)：
1. data do not support the hypothesis that retinal itself represses weight gain and adipogenesis independently of RA. Instead, the data indicate that Raldh1 functions as a retinal and atRA-independent promoter of adiposity during adolescence, and enhances adiposity through pre-adipocyte cell autonomous actions PMID: 29095919
2. these studies identify Notch signaling in dendritic cells as a crucial balancer of Th17/iTreg, which depends on the direct regulation of Aldh1a2 transcription in dendritic cells PMID: 28779023
3. ALDH2 mutation displays an inverse correlation of coronary collateral vessel formation in patients. PMID: 26315408
4. Data suggest that retinoic acid and GM-CSF-induced retinal dehydrogenase 2 (RALDH2) expression in dendritic cells requires cooperative binding of transcription factor Sp1 via the RA receptor/retinoid X receptor complex to the Aldh1a2 promoter. PMID: 24788806
5. Defects in interdigital programmed cell death and digit separation in Hoxa13 mutant mice may be caused in part by reduced levels of RA signaling stemming from a loss in the direct regulation of Aldh1a2 PMID: 23553814
6. Raldh1 and Raldh3 influence enteric nervous system structure and function and heterozygosity for Raldh2 causes ENS defects PMID: 23806210
7. ALDH1A2 expression was highest in ALDH(very-br) cells, intermediate in ALDH(dim) cells, and lowest in ALDH(br) cells. PMID: 23484127
8. Upregulation of retinal dehydrogenase 2 in alternatively activated macrophages during retinoid-dependent type-2 immunity to helminth infection in mice. PMID: 22927819
9. rendered Fgfr2IIIb(-/-) embryos haploinsufficient for the Raldh2 and examined these embryos for the incidence and severity of duodenal atresia PMID: 23021139
10. In Fgfr2IIIb-/- mouse embryos, a reduction of Raldh2 expression is observed within the duodenal region that is forming the atresia. PMID: 21492869
11. regulation of fat depots through the concerted action of Aldh1 enzymes establishes retinoic acid-dependent tandem regulation of transcription factors ZFP423 and PPARgamma in a depot-specific manner PMID: 21436255
12. Analysis of Ret, Gata3 or Raldh2 mutant mice at birth reveals hydronephrosis and defective ureter maturation, abnormalities that our results suggest are caused, at least in part, by delayed insertion of the nephric duct. PMID: 21521737
13. Wt1 controls retinoic acid signalling in embryonic epicardium through transcriptional activation of Raldh2. PMID: 21343363
14. The heart phenotypes of Raldh2 mutants are very similar and are characterized by a prominent defect in ventricular compact zone growth. PMID: 21138976
15. These findings suggest that an increasing expression of Raldh3 deregulates the balanced mechanisms of insulin and glucagon secretion in the pancreatic islets and may induce beta-cell dysfunction leading to the development of type 2 diabetes. PMID: 20833146
16. Drastic down-regulation of Aldh1a2 observed at both E16 and E18; RT-PCR revealed post-natal reduction in expression (Aldh1a2, 1/13). Results suggest down-regulation of gene is important factor in normal odontogenesis in dental papillae. PMID: 20448247
17. limited to the digit-interdigit junction rather than being expressed throughout the interdigital zone PMID: 20034106
18. raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception. PMID: 20081195
19. Raldh2 and Raldh3 are selectively expressed in cortical stroma and in the ureteric bud during kidney development. Renal development depends mainly on Raldh2. PMID: 20040494
20. Three retinaldehyde dehydrogenases (RALDH1, RALDH2 and RALDH3), show differential expression patterns throughout later mouse organogenesis PMID: 11744377
21. Aldh1a2 haploinsufficiency prevents the appearance of spina bifida and rescues the development of posterior structures. PMID: 11953746
22. Novel retinoic acid generating activities in the neural tube and heart identified by conditional rescue of Raldh2 null mutant mice. PMID: 11959834
23. RALDH2 is responsible for most of the patterns of reporter transgene activity in the spinal cord and trunk mesodermal derivatives. Independent pathways of retinoic acid synthesis may also exist. PMID: 12454286
24. RALDH2 plays a crucial role in producing RA (retinoic acid) required for pharyngeal development, and RA is one of the diffusible mesodermal signals that pattern the pharyngeal endoderm. PMID: 12702665
25. In knockout mice, retinoic acid plays a crucial role in mammalian vascular development; it is required to control endothelial cell proliferation and vascular remodeling during vasculogenesis. PMID: 14627725
26. Expression of retinaldehyde dehydrogenase II in the embryonic mouse caudal hindbrain. PMID: 15053971
27. Raldh2 has a role in retinoic acid synthesis for limb bud initiation and then later as a proximodistal signal during apical ectodermal ridge formation PMID: 15069081
28. The expression patterns of aldh1a2 and aldh1a3 retinoic acid synthesizing enzymes at specific follicular sites suggest that they mediate and are regulated by different epithelial proliferation and differentiation signaling pathways. PMID: 15245423
29. catalysis study of retinaldehyde dehydrogenase type II PMID: 15299009
30. Data show that retinaldehyde dehydrogenase (Raldh)2-/- embryos lacking retinoic acid synthesis in the optic vesicle exhibit a failure in retina invagination needed to develop an optic cup. PMID: 15366004
31. Raldh2 has a role in posterior neural transformation through generation of mesodermal retinoic acid PMID: 15652703
32. Mutant embryos display an early embryonic loss of a subset of Lim1+ brachial motoneurons PMID: 15753214
33. Raldh2 and Cyp26 generate shifting boundaries of retionic acid activity, controlling hindbrain segmental gene expression. PMID: 15872003
34. RALDH-2-deficient mice do not develop a dorsal pancreatic bud. PMID: 16026781
35. We have identified the retinoic acid synthesis enzyme Aldh1a2 gene as a principal target of bone morphogenetic protein (BMP) signaling; prochondrogenic BMPs or growth-differentiation factors attenuate Aldh1a2 expression and the retinoid signaling pathway PMID: 16818722
36. Aldh1a2 transcripts and its protein RALDH2 began to increase in the testis at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. PMID: 18478160
37. RARalpha/retinoid X receptor beta (RXRbeta) complex binds to the mouse retinaldehyde dehydrogenase 1 (Raldh1) promoter at a non-consensus retinoic acid response element. PMID: 18992716
38. Restricted expression during ontogenesis may correspond to specific sites of RA synthesis during inner ear morphogenesis. PMID: 11472854
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Aldehyde dehydrogenase family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:19378

STRING: 10090.ENSMUSP00000034723

UniGene: Mm.42016