Recombinant Mouse Protein piccolo (Pclo), partial

中文名稱：

小鼠Pclo重組蛋白
貨號：

CSB-YP886191MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Pclo重組蛋白
貨號：

CSB-EP886191MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Pclo重組蛋白
貨號：

CSB-EP886191MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Pclo重組蛋白
貨號：

CSB-BP886191MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Pclo重組蛋白
貨號：

CSB-MP886191MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Pclo
Uniprot No.：

Q9QYX7
別名：

Pclo; Acz; Protein piccolo; Aczonin; Brain-derived HLMN protein; Multidomain presynaptic cytomatrix protein
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released. After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts. At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release. Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy. Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import.
基因功能參考文獻：
1. Piccolo puncta sandwiched Bassoon puncta and aligned in a Piccolo-Bassoon-Piccolo structure in adult neuromuscular junctions (NMJs). P/Q-type voltage-gated calcium channel (VGCC) puncta colocalized with Bassoon puncta. The P/Q-type VGCC and Bassoon protein levels decreased significantly in NMJs from aged mouse. In contrast, the Piccolo levels in NMJs from aged mice were comparable to levels in adult mice. PMID: 27321892
2. a function of Piccolino at ribbon synapses of sensory neurons PMID: 23936420
3. a novel role for Bassoon and Piccolo as critical regulators of presynaptic ubiquitination and proteostasis. PMID: 23403927
4. Piccolo is an important regulator of presynaptic F-actin, functioning to coordinate its activity-dependent assembly and thereby modulating neurotransmitter release. PMID: 21976510
5. piccolo and bassoon play a redundant role in synaptic vesicle clustering in nerve terminals without directly participating in neurotransmitter release. PMID: 20332206
6. Piccolo plays a pivotal role in synaptic plasticity in area CA1 and in hippocampus-dependent learning in mice; the extracellular levels of glutamate in the hippocampus under stimulated conditions are controlled by Piccolo. PMID: 19766155
7. binds to cAMP-GEFII and forms both homodimer and heterodimer with Rim2 in a Ca(2+)-dependent manner, whereas Rim2 alone does not form the homodimer PMID: 12401793
8. These results suggest that Piccolo is a components of neuromuscular junction synapses in the peripheral nervous system. PMID: 17435594
9. Piccolo C2A domain may act as a cytoskeletal regulator for plasmalemmal DAT internalization, which may underlie its contributions in behavioral plasticity. PMID: 18195717
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亞細胞定位：

Cell junction, synapse, presynaptic active zone.
組織特異性：

Highly expressed in brain. Moderately expressed in pituitary gland and pancreatic islets. Low levels found in stomach.
數據庫鏈接：

KEGG: mmu:26875

STRING: 10090.ENSMUSP00000030691

UniGene: Mm.146275