Recombinant Mouse Pleiotrophin (Ptn)

中文名稱：

小鼠Ptn重組蛋白
貨號：

CSB-YP019009MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Ptn重組蛋白
貨號：

CSB-EP019009MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Ptn重組蛋白
貨號：

CSB-EP019009MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ptn重組蛋白
貨號：

CSB-BP019009MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ptn重組蛋白
貨號：

CSB-MP019009MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ptn
Uniprot No.：

P63089
別名：

Ptn; Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neutrophic factor; HBNF; Osteoblast-specific factor 1; OSF-1
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

33-168
氨基酸序列

GKKEKPEK KVKKSDCGEW QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK YQFQAWGECD LNTALKTRTG SLKRALHNAD CQKTVTISKP CGKLTKPKPQ AESKKKKKEG KKQEKMLD
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK or AFAP1L2 in order to activate the PI3K-AKT pathway. Through PTPRZ1 binding controls oligodendrocyte precursor cell differentiation by enhancing the phosphorylation of AFAP1L2 in order to activate the PI3K-AKT pathway. Forms a complex with PTPRZ1 and integrin alpha-V/beta-3 (ITGAV:ITGB3) that stimulates endothelial cell migration through SRC dephosphorylation and activation that consequently leads to ITGB3 'Tyr-773' phosphorylation. In adult hippocampus promotes dendritic arborization, spine development, and functional integration and connectivity of newborn granule neurons through ALK by activating AKT signaling pathway. Binds GPC2 and chondroitin sulfate proteoglycans (CSPGs) at the neuron surface, leading to abrogation of binding between PTPRS and CSPGs and neurite outgrowth promotion. Binds SDC3 and mediates bone formation by recruiting and attaching osteoblasts/osteoblast precursors to the sites for new bone deposition. Binds ALK and promotes cell survival and cell proliferation through MAPK pathway activation. Inhibits proliferation and enhances differentiation of neural stem cells by inhibiting FGF2-induced fibroblast growth factor receptor signaling pathway. Mediates regulatory mechanisms in normal hemostasis and in hematopoietic regeneration and in maintaining the balance of myeloid and lymphoid regeneration. In addition may play a role in the female reproductive system, auditory response and the progesterone-induced decidualization pathway.
基因功能參考文獻：
1. Molecular crosstalk between tumour-associated macrophages and glioma stem cells through PTN-PTPRZ1 paracrine signalling supports glioblastoma malignant growth. PMID: 28569747
2. PTN expression in glioma cells is associated with chromosome 7 gain. PTN enhances PDGFB-induced gliomagenesis by stimulating proliferation of neural progenitor cells. PMID: 27806344
3. The data demonstrate that PTN overexpression in the brain blocks the conditioning effects of amphetamine and enhances the characteristic striatal dopaminergic denervation caused by this drug PMID: 27642078
4. Ptn may play a vital role in the progesterone-induced decidualization pathway via C/EBPB-cyclic AMP-Hand2 signaling. PMID: 28657144
5. The results of this study suggested that regulation of the PTN signaling pathways may constitute new therapeutic opportunities particularly in those neurological disorders characterized by increased PTN cerebral levels and neuroinflammation. PMID: 28259175
6. these data indicate that PTN-PTPRZ-A signaling controls the timing of oligodendrocyte precursor cell differentiation in vivo, in which the CS moiety of PTPRZ receptors maintains them in a monomeric active state until its ligand binding. PMID: 27445335
7. findings link pleiotrophin abundance in gliomas with survival in humans and mice, and show that pleiotrophin promotes glioma progression through increased VEGF deposition and vascular abnormalization. PMID: 26645582
8. antagonizes Brd2 promoting cell differentiation associated with the neural tube: spinal cord neurogenesis and neural crest migration PMID: 24695857
9. PTN-mediated hematopoietic regeneration occurs in a RAS-dependent manner. PMID: 25250571
10. the absence of PTN in vivo is associated with disruption of specific cognitive and affective processes PMID: 25000129
11. The results of this study indicated that PTN may be a novel genetic factor of importance in alcohol use disorders. PMID: 25073406
12. data suggest that the spinal antinociceptive effects caused by stimulation of alpha2-adrenoceptors are differentially regulated by endogenous expression of PTN PMID: 23891830
13. PTN overexpression exerts various effects on sarcolemma ion channel expression/function and resting cytosolic calcium concentrations in soleus and extensor digitorum longus muscles. PMID: 24015201
14. Vivarium-housed Wt controls had higher levels of active TGF-beta1 and IL-2 in inguinal lymph nodes relative to PTN-Tg mice. PMID: 23874826
15. Pleiotrophin exerts a neuroprotective role against amphetaine-induced neurotoxicity in striatum. PMID: 23459167
16. the data demonstrate that maintenance of amphetamine-induced conditioning effects depend on the endogenous expression of PTN. PMID: 23178526
17. We conclude that PTN delays the maturation of the mammary gland by maintaining mammary epithelial cells in a progenitor phenotype and by inhibiting their differentiation during mammary gland development. PMID: 23077670
18. PTN is a secreted component of the bone marrow vascular niche that regulates hematopoietic stem cell self-renewal and retention in vivo. PMID: 23084748
19. Data suggest a physiological role for HARP in modulating (i.e., antagonizing) endogenous anticoagulant activity of heparin-like material in the vasculature. PMID: 22672269
20. alterations of the bone microarchitecture that occurred in wild type and pleiotrophin-transgenic mice exposed to a near-zero gravity on the International Space Station PMID: 22438896
21. PTN is widely expressed during tooth development and also in the adult mouse molar and incisor. PMID: 22382872
22. this study shows that PTN expression is upregulated in a mouse model of peritoneal fibrosis and is present in human peritoneal tissues and in peritoneal dialysate effluent. PMID: 21881556
23. MiR-143 down regulates PTN expression through interaction with a target site of miR-143 in the coding region of mouse PTN. PMID: 21945314
24. Gene inactivation of Pleiotrophin results in enhanced amphetamine neurotoxicity in the striatum. PMID: 20620199
25. The data suggest an important role of PTN to limit amphetamine-induced neurotoxic and rewarding effects. PMID: 20192945
26. regulates expansion and regeneration of hematopoietic stem cells PMID: 20305662
27. Mice overexpressing HB-GAM learned faster in mazes and showed decreased anxiety, while HB-GAM knockouts showed opposite behavior. HB-GAM may suppress LTP in hippocampus and influence learning-related behavior. PMID: 12093164
28. role of anaplastic lymphoma kinase and pleiotrophin signaling in apoptosis PMID: 12107166
29. Pleiotrophin mRNA is strongly induced in the brain by day 7 after injury, which correlates with the expression pattern of syndecan-3 mRNA. PMID: 12112370
30. we propose that PTN is an accessory signaling molecule, which is involved in a variety of processes in bone formation PMID: 12412809
31. roles during in vivo bone formation and remodeling, probably acting as a co-factor or accessory protein that modulates the effects of primary signaling molecules PMID: 12413943
32. HB-GAM functions as an endogenous inhibitor of FGF-2 in stem cell proliferation in the developing cortex. PMID: 15121180
33. PTN mRNA is highly expressed in neural stem (progenitor) cells of mouse ventral mesencephalon. PTN promotes the production of DAergic neurons from embryonic-stem-cell-derived nestin-positive cells. PMID: 15180956
34. The mRNA transcripts of PTN and its receptors were detected in neurosphere, suggesting that pleiotrophin signaling systems are present in the neural stem cells and are involved in the modulation of fate of neural stem cells. PMID: 15351507
35. These findings suggest that Ptn gene expression has a critical role in determining the levels of expression of the enzymes of catecholamine biosynthesis in aorta. PMID: 15369781
36. Ptn has the potential to critically regulate the downstream activities of angiotensin II, through the regulation of its synthesis by ACE and its receptor mediated functions through regulation of both the AT1 and AT2 receptors PMID: 15485659
37. Ptn-deficient mice do not display detectable defects in endochondral and intramembranous ossification and intramembranous ossification or bone remodeling. PMID: 15589206
38. Together, these results demonstrate that influence of CNTF family of cytokines on the differentiation of late retinal progenitor cell population is partially mediated by the release of Ptn. PMID: 16914133
39. Female infertility in mice may result from deficiency in Ptn. PMID: 17121547
40. Knocking down the expression of PTN using siRNA resulted in an increase in phospho-GSK-3beta expression, and the accumulation of nuclear beta-catenin, which are critical downstream signaling proteins for both the PTN and Wnt signaling pathways. PMID: 17239862
41. Pleiotrophin is a neurotrophic factor for spinal motor neurons PMID: 17360581
42. Inhibition of the mitogenic, angiogenic, and tumorigenic activities of Ptn by a synthetic peptide corresponding to its C-thrombospondin repeat-I domain. PMID: 17607711
43. Pleiotrophin is secreted from fibroblasts and has an antiapoptotic effect in these cells and in embryonic stem cells. PMID: 17823238
44. Data show that N-syndecan, the receptor for the extracellular matrix component heparin-binding growth-associated molecule, is required for survival of primary sensory neurons. PMID: 18766019
45. The results suggest that HB-GAM is an osteocyte-derived factor that could participate in mediating the osteogenic effects of mechanical loading on bone. PMID: 19442624
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亞細胞定位：

Secreted.
蛋白家族：

Pleiotrophin family
組織特異性：

Osteoblast and brain. Expressed in the follicular epithelium and granulosa cells of the ovary. Strongly expressed in the uterus of newborn mice, and the degree of expression decreased in one-week-old mice, although the expression continues even in the ute
數據庫鏈接：

KEGG: mmu:19242

STRING: 10090.ENSMUSP00000099073

UniGene: Mm.279690