Recombinant Mouse Nuclear factor NF-kappa-B p100 subunit (Nfkb2), partial
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中文名稱：小鼠Nfkb2重組蛋白
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貨號：CSB-EP896226MO-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Nfkb2; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2) [Cleaved into: Nuclear factor NF-kappa-B p52 subunit]
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種屬：Mus musculus (Mouse)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
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基因功能參考文獻：
- in vivo bioluminescence imaging in a transgenic model of inflammation showed that luciferase activity coincided with endogenous p52/p100 Nfkb2 expression PMID: 29680659
- our studies for the first time establish p100 as a key tumor suppressor of bladder cancer growth PMID: 27095572
- hyperresponsive promoter engaged the RelA:p52 dimer generated during costimulation of macrophages through TLR4 and LTbetaR to trigger synthesis of IkappaBalpha at late time points, which prevented the late-acting RelA cross-talk response. PMID: 27923915
- results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
- Results suggest that the RelB/NF-kappaB2 pathway regulates T cell migration to autoimmune targets in Sjogren syndrome through TGFbeta/TGFbetaR-dependent regulation of CXCL12/CXCR4 signaling. PMID: 28804991
- MKK4 activates non-canonical NFkappaB signaling by promoting NFkappaB2-p100 processing. PMID: 28733031
- the aberrant proliferative capacity of Brca1(-/-) luminal progenitor cells is linked to the replication-associated DNA damage response, where proliferation of mammary progenitors is perpetuated by damage-induced, autologous NF-kappaB signaling. PMID: 27292187
- RelB is processed by CO2 in a manner dependent on a key C-terminal domain located in its transactivation domain. Loss of the RelB transactivation domain alters NF-kappaB-dependent transcriptional activity, and loss of p100 alters sensitivity of RelB to CO2 PMID: 28507099
- we identify in this study a critical role for the combined activity of the RELB and NF-kappaB2 subunits in B cell homeostasis that cannot be compensated for by the canonical NF-kappaB pathway under physiological conditions. PMID: 26851215
- Mechanistically the LncRNA-HIT siRNA treatments impacted pro-chondrogenic gene expression by reducing H3K27ac or p100 activity, confirming that LncRNA-HIT is essential for chondrogenic differentiation in the limb mesenchyme PMID: 26633036
- Nfkb2 connected lymphotoxin signal within the intestinal niche in reinforcing epithelial innate inflammatory RelA/NF-kappaB response to Citrobacter rodentium infection, while Nfkb2(-/-) mice succumbed to gut infections owing to stromal defects. PMID: 25905673
- Data indicate a coupled increase in the expression of renal, relA, NF-kB2, and p53 genes and proteins during folic acid induced acute kidney injury (AKI). PMID: 25559736
- Loss of c-REL but not NF-kappaB2 prevents autoimmune disease driven by FasL mutation. PMID: 25361085
- NF-kappaB2/p52 is necessary for the development of colitis, whilst c-Rel-mediated signalling regulates colonic epithelial cell turnover following DNA damage. PMID: 25727407
- Nuclear translocation of c-Rel was enhanced in p100-deficient cells. p100, and not the processed p52 form, associated with c-Rel in the steady state and dissociated immediately after lipopolysaccharide stimulation in wild-type dendritic cells PMID: 25305492
- The unique ability of p100/IkappaBdelta to stably interact with all NF-kappaB subunits by forming kappaBsomes demonstrates its importance in sequestering NF-kappaB subunits and releasing them as dictated by specific stimuli for developmental programs. PMID: 25349408
- that p52 binds to the promoter of the GM-CSF-encoding gene (Csf2) and cooperates with c-Rel in the transactivation of this target gene. PMID: 24899500
- NF-kappaB p65/p52 signaling mediated the effects of GDNF on Bcl-2 and Bcl-w expressions PMID: 23590664
- Heterozygous mutations in NFKB2 cause a unique form of early-onset CVID that also presents with central adrenal insufficiency. PMID: 24140114
- p100 deficiency alone was insufficient for full induction of genes regulated by the alternative NF-kappaB pathway. PMID: 22880094
- RelB/NF-kappaB2 signaling positively and intrinsically regulates hematopoietic stem/progenitor cell self-renewal. PMID: 22290873
- the alternative the NF-kappaB pathway via the processing of p52 from p100 negatively regulates osteoblastic differentiation and bone formation by modifying bone morphogenetic protein activity. PMID: 22282470
- The p52/RelB noncanonical pathway is critical to sustain CXCL12/SDF1 production in order for cells to migrate toward chromatin architectural protein HMGB1. PMID: 22287708
- Nuclear factor-kappaB2/p100 promotes endometrial carcinoma cell survival under hypoxia in a HIF-1alpha independent manner. PMID: 21537326
- findings demonstrate the physiological relevance of the NF-kappaB2 p100 precursor protein in limiting the potentially detrimental effects of constitutive NF-kappaB2 signaling in lymphocytes PMID: 20363924
- Mice that were deficient in the alternative NF-kappaB pathway subunit p52 showed an impaired T-cell response against lymphocytic choriomeningitis virus infection. PMID: 20565293
- Results show that TNF limits RANKL- and TNF-induced osteoclast formation in vitro and in vivo by increasing NF-kappaB p100 protein accumulation in osteoclast precursors. PMID: 19770515
- Nfkb2 knockout mice develop chronic trichuriasis associated with elevated production of antigen-specific IFN-gamma but can recover IL-13 responses and resistance to infection if IL-12 responses are blocked. PMID: 12370384
- ligation of CD40 on transfected 293 cells is shown to trigger p52 production by stimulating p100 ubiquitylation and subsequent proteasome-mediated proteolysis PMID: 12374738
- mice injected with BAFF-neutralizing BR3-Fc protein showed reduced basal NF-kappaB2 activation PMID: 12387744
- NF-kappa B2 p100 has I kappa B-independent apoptotic activity and a unique tumour suppressor role PMID: 12389034
- role in Peyer's patch development along with RelB and p50, focusing on lymphotoxin and tumor necrosis factor signal transduction PMID: 12505990
- RelB has an effect on p100 processing, which is possibly regulated in a signal-dependent manner PMID: 12874295
- controls osteoclatogenesis PMID: 12939342
- NF-kappaB2 represses RelB in dendritic cells (DC) by a regulatory pathway that may function to prevent DC hyperactivation, with critical consequences for the regulation of adaptive immune responses. PMID: 14707043
- Results identify a novel type of NF-kappaB-binding site in IkappaB kinase alpha-dependent chemokine gene promoters that is preferentially recognized by RelB:p52 dimers. PMID: 15470505
- IkappaB kinase alpha has an essential role in the constitutive processing of NF-kappaB2 p100 PMID: 15677466
- RelB/p52 complexes have roles in mammary gland development and carcinogenesis PMID: 16260626
- CD14-mediated LPS signaling pathway may play a role in the regulation of NF-kappaB alternative splicing in the lungs after injury PMID: 16480837
- Akt plays a role in activation of basal and induced non-canonical NF-kappaB activity PMID: 16613850
- NF-kappaB2 (p100) limits nuclear translocation of NF-kappaB1-RelA and thereby functions as a regulatory 'brake' for the activation of naive T cells. PMID: 16732290
- NF-kappaB2 downstream of lymphotoxin beta receptor plays an important role in the regulation of central tolerance in an autoimmune regulator-dependent manner PMID: 17039258
- findings identify a physiological function of NF-kappaB2 in the development of medullary thymic epithelial cells and, thus, the control of self-tolerance induction PMID: 17046818
- Our genetic evidence confirms that p100 is required and sufficient as a fourth IkappaB protein for noncanonical NF-kappaB signaling downstream of NIK and IKK1 PMID: 17254973
- Increased p100/p52 disrupts normal mammary gland ductal development by a mechanism which may contribute to human breast cancer. PMID: 17261585
- activation of NF-kappaB2/p100 plays a crucial role in the Tax1-mediated transformation of T cells PMID: 17715223
- production of NF-kappaB2 p52 is not tumorigenic but predisposes mice to inflammatory autoimmune disease by repressing Bim expression PMID: 18281283
- inhibition of TRAF3 results in coordinated activation of both the p50-dependent and p52-dependent NF-kappaB activation pathways PMID: 18292232
- tightly controlled p100 processing and RelB activation is essential for normal B lymphopoiesis and lymphoid/myeloid lineage decision in bone marrow PMID: 18505785
- RIG-1 - MAVS interacts with cytoplasmic 100-kDa NF-kappa B2 complexes via a novel retinoic acid-inducible gene-I - NF- kappa B-inducing kinase signaling pathway PMID: 18550535
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亞細胞定位：Nucleus. Cytoplasm.
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組織特異性：Highly expressed in lymph nodes and thymus.
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