Recombinant Mouse NAD (+) hydrolase SARM1 (Sarm1), partial

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中文名稱：

小鼠Sarm1重組蛋白
貨號(hào)：

CSB-EP747471MO
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Sarm1
Uniprot No.：

Q6PDS3
別名：

(NADase SARM1)(Sterile alpha and TIR motif-containing protein 1)
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
來源：

E.coli
分子量：

40.7 kDa
表達(dá)區(qū)域：

409-705aa
氨基酸序列

VASWKEAEVQTWLQQIGFSQYCENFREQQVDGDLLLRLTDEELQTDLGMKSSITRKRFFRELTELKTFASYATCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIRLGVHRTRILSAAREMLHSPLPCTGGKLSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVIAARNFVLVLSAGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQALPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRPSQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
基因功能參考文獻(xiàn)：
1. Sarm1 deletion, but not Wld(S), confers lifelong rescue in a mouse model of severe axonopathy. PMID: 28978465
2. This study extends the role of Sarm1 to axon degeneration seen in peripheral neuropathies and identifies it as a likely target for therapeutic development. PMID: 28485482
3. SARM1 deletion restrains non-alcoholic fatty liver disease induced by high fat diet (HFD) in mice. PMID: 29454967
4. we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644
5. Using steady-state and flux analysis of NAD(+) metabolites in healthy and injured mouse dorsal root ganglion axons, we find that rather than altering NAD(+) synthesis, NMNAT1 instead blocks the injury-induced, SARM1-dependent NAD(+) consumption that is central to axon degeneration. PMID: 27735788
6. demonstrate that the NADase activity of full-length SARM1 is required in axons to promote axonal NAD+ depletion and axonal degeneration after injury. PMID: 28334607
7. Genetic deletion of SARM1 decreases axonal degeneration in a mouse model of neuropathy. PMID: 27797810
8. SARM is a potential regulator of sepsis-induced splenocyte apoptosis. PMID: 27590237
9. Sarm1(-/-)mice developed fewer Beta-amyloid precursor protein aggregates in axons of corpus callosum after traumatic brain injury. PMID: 26912636
10. SARM1-induced depletion of NAD(+) may explain the potent axon protection in Wallerian degeneration slow (Wld(s)) mutant mice. PMID: 25908823
11. The findings suggest that Sarm1 regulates social behaviors and cognition. PMID: 24321214
12. knock-outs display altered inflammatory cytokine expression pattern in the brain neurons PMID: 23751821
13. Therefore, Sarm1 functions downstream of ROS to induce neuronal cell death and axon degeneration during oxidative stress. PMID: 25009267
14. this study identifies a new role for SARM in CCL5 expression in macrophages. PMID: 24711619
15. Wild-type LPS is able to upregulate SARM and to prevent SIRPalpha downregulation. PMID: 23836818
16. These results indicate that SARM plays an integral role in the dismantling of injured axons PMID: 23946415
17. data demonstrate SARM plays a role in neurodegeneration during viral CNS infection; in addition, SARM plays a positive role in cytokine production; data suggest SARM is crucial for CNS injury and cytokine production in the CNS and may provide a link between neurodegeneration and the innate immune response PMID: 23749635
18. Results presented in this study, for the first time, show that KA-mediated upregulation of SARM1 protein promotes Wallerian-like degeneration of retinal ganglion cells and their axons. PMID: 23518770
19. severed mouse Sarm1 null axons exhibit long-term survival both in vivo and in vitro, indicating Sarm1 prodegenerative signaling is conserved in mammals; results provide direct evidence that axons actively promote their own destruction after injury and identify dSarm/Sarm1 as a member of an ancient axon death signaling pathway PMID: 22678360
20. The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856
21. Burkholderia pseudomallei modulates macrophage defense mechanisms by upregulating SARM, thus leading to the suppression of IFN-beta and iNOS needed for bacterial elimination PMID: 21555400
22. Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphogenesis. PMID: 21555464
23. SARM functions to restrict viral infection and neuronal injury in a brain region-specific manner, possibly by modulating the activation of resident CNS inflammatory cells. PMID: 19587044
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亞細(xì)胞定位：

Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
組織特異性：

Widely expressed in the brain and neurons (at protein level). Expressed in photoreceptor cells of the neural retina.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:237868

UniGene: Mm.210332