E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
功能:
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9. Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
基因功能參考文獻:
data therefore reveal a role for NMIIA and NMIIB as negative regulators of proliferation in the mammary epithelium. PMID: 28821574
A novel mechanism linking epicardial formation and epicardial function to the activity of the cytoplasmic motor protein NMIIB. PMID: 28687623
Positional cloning and candidate gene analysis revealed that the EHC phenotype results from a point mutation in a splice donor site of the Myh10 gene, which encodes NMHC IIB. Our studies on the EHC mutant demonstrate a requirement for NMHC IIB in epicardial function and coronary vessel formation, highlighting the importance of this protein in cardiac development and ultimately, embryonic survival. PMID: 29084269
Data show that deletion of myosin heavy chain II-A (Myh9)/myosin heavy chain II-B (Myh10) caused severe hydroureter/hydronephrosis at birth. PMID: 28478097
cytokinesis failure in megakaryocytes is the consequence of both the absence of NMIIB and a low RhoA activity that impairs NMIIA localization at the cleavage furrow through increased actin turnover. PMID: 27737892
The experimental and in silico results suggest two divergent evolutionary pathways: NMIIA and NMIIB evolved to possess S100A4-dependent and -independent regulations, respectively. PMID: 27029887
The novel finding that calponin 2 regulates myosin-dependent CTF in non-muscle cells demonstrates a mechanism for controlling cell motility-based functions PMID: 27733037
Study suggests that NMIIB remains constitutively active in support of methamphetamine associations, continuing to drive actin polymerization, such that memories associated with methamphetamine can be selectively targeted without the need for retrieval days to weeks after learning PMID: 26239291
A central biophysical role for NMIIB in nuclear translocation during 3D invasive migration. PMID: 26261182
We have discovered that NMIIB is the major isoform regulating Schaffer collateral inputs, and that this regulation is restricted to early postnatal development. PMID: 25931194
regulates morphogenesis of immature nephrons PMID: 25168025
These results supported the hypothesis that, like NMHC-IIA, NMHC-IIB associated with herpes simplex virus 1 gB and mediated herpes simplex virus 1 entry. PMID: 25428876
Point mutation in Myh10 causes major defects in heart development and ventral body wall closure. PMID: 24825879
The C-terminal domain of MYH10 is responsible for the specific localization of myosin II in megakaryocyte contractile ring. PMID: 25185263
The results of this study propose a pivotal role of NM II in cytoskeleton organization during microglial activation. PMID: 25150163
This study reveals divergent functions for the 2 Myo10 isoforms in controlling both the direction of migration and neuronal morphogenesis during radial cortical neuronal migration. PMID: 23300110
The data validate the function of NMII as that of a negative regulator of OL myelination in vivo and provide a novel target for promoting myelin repair in conditions such as multiple sclerosis. PMID: 24470341
emerin functions with myosin IIB to polarize actin flow and nuclear movement in fibroblasts, suggesting a novel function for the nuclear envelope in organizing directional actin flow and cytoplasmic polarity. PMID: 24152738
These results indicate that MIIB-driven tension or actin dynamics regulate the major pathway for synaptic vesicle retrieval PMID: 24107946
NMII plays a role in T-cell polarity and is regulated by upstream signals. PMID: 23818610
NMIIB is required for IRE1-alpha aggregation and foci formation under endoplasmic reticulum stress PMID: 23237951
In spite of the presence of multiple non-muscle myosin II isoforms, we demonstrate that a single member, NMIIB, plays an essential and non-redundant role in cytokinesis during meiotic cell divisions of the male germline. PMID: 22820068
RUNX1-mediated silencing of MYH10 is required for the switch from mitosis to endomitosis, linking polyploidization with megakaryocyte differentiation. PMID: 22395608
that the major roles of NM II in vertebrate cell cytokinesis are to bind and cross-link actin filaments and to exert tension on actin during contractile ring constriction PMID: 22393000
MHCIIB deficiency causes imbalances in signalling pathways that are responsible for cell polarity determination. PMID: 21785947
Data show that CXCL13/CXCR5 facilitated antigen encounter and BCR signaling by promoting membrane ruffling and LFA-1-supported adhesion require a functional actin cytoskeleton and the activity of the motor protein non-muscle myosin II (NM-II). PMID: 21659539
myosin IIA and IIB are differentially expressed and localized and have clearly different functions in hepatic stellate cells PMID: 20932596
Ozz-E3 as the ubiquitin ligase complex that interacts with and regulates myosin within its fully assembled cytoskeletal structure PMID: 20352047
The migration defect in Cln3(-/-) results, in part, from the loss of the CLN3-myosin-IIB interaction. PMID: 20850431
Loss of myosin II, VI or VIIa function had no significant effects on rates of endocytic vesicle movement in bone marrow-derived dendritic cells.[myo2a or Non muscle myosin II] PMID: 17615572
Results highlight the functions of the N-terminal motor and C-terminal rod domains of NM II and their different roles in cell-cell and cell-matrix adhesion. PMID: 20679233
The mRNA levels for myosin IIb increased significantly between 5 and 7 weeks in developing postnatal mice. PMID: 20166551
cell volume perturbations are associated with changes in myosin IIB distribution. PMID: 12061817
the forward advance of the growth cone is myosin II dependent and involves multiple myosin II isoforms. PMID: 12584251
Myosin IIB is involved not in propelling but in directing the cell movement, by coordinating protrusive activities and stabilizing the cell polarity PMID: 14699073
NMHC II-B is particularly important for normal migration of distinct groups of neurons during mouse brain development PMID: 15034141
we suggest that cyclic myosin II-B assembly and contraction in lamellipodia power 3D fibre movements. PMID: 15654332
Both myosin IIA and myosin IIB are localized in nerve terminals. PMID: 16012337
the presence of different alternatively 5'-spliced smooth muscle-myosin heavy chain (SM-MHC) isoforms does not dominate the different contractile features of physiologically loaded renal afferent or efferent arterioles PMID: 16316347
Deletion of the B1 alternative exon, together with reduced expression of myosin II-B, results in abnormal migration and consequent protrusion of facial neurons into the fourth ventricle. PMID: 16481398
Data suggest that non-muscle myosin 2B is important for bipolar shape formation and adhesion owing to its preferential interaction with membrane-associated actin, and the role myosin 2B in retraction prevents over-elongation of myoblasts PMID: 16895968
Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation. PMID: 17389233
These studies show that the scaffolding properties of NM II-B play an important role in cell adhesion, thereby preventing hydrocephalus during mouse brain development. PMID: 17429076
Replacement of Myh2a with myosin IIB rescues brain from hydrocephalus and manttains integrity of the spinal canal. PMID: 17519229
downregulation of myosin II-B, the major myosin isoform in neurons, is able to increase Abeta deposition, concomitantly altering the subcellular localization of APP PMID: 17727819
The results show that NMHC IIB knockdown led to decreased N-RAP levels through proteasome-mediated degradation. PMID: 18615632
Expression of full-length human NMHC-IIA and -IIB in 10 T1/2 cells demonstrated that flectin antibody recognizes both isoforms PMID: 18697221
The response of dorsal root ganglion (DRG) neurons to Semaphorin 3A gradients can be divided into two steps: growth cone collapse and retraction. Collapse is inhibited by overexpression of myosin IIA or growth on high substrate-bound laminin-1. PMID: 19109430
myosin II, directly regulates cellular patterning and alignment within the cochlear sensory epithelium. PMID: 19439495
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亞細胞定位:
Cell projection, lamellipodium.
蛋白家族:
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
組織特異性:
In newborn kidney, expressed in the mesenchyme and ureteric buds.