Recombinant Mouse L-dopachrome tautomerase (Dct), partial

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中文名稱：

Recombinant Mouse L-dopachrome tautomerase(Dct),partial
貨號(hào)：

CSB-EP006562MO1
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Dct
Uniprot No.：

P29812
別名：

Dct; Tyrp-2; Tyrp2; L-dopachrome tautomerase; DCT; DT; EC 5.3.3.12; DOPAchrome conversion factor; DOPAchrome isomerase; DOPAchrome oxidoreductase; L-dopachrome Delta-isomerase; SLATY locus protein; Tyrosinase-related protein 2; TRP-2; TRP2
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
來(lái)源：

E.coli
分子量：

69.7kDa
表達(dá)區(qū)域：

24-472aa
氨基酸序列

QFPRVCMTLDGVLNKECCPPLGPEATNICGFLEGRGQCAEVQTDTRPWSGPYILRNQDDREQWPRKFFNRTCKCTGNFAGYNCGGCKFGWTGPDCNRKKPAILRRNIHSLTAQEREQFLGALDLAKKSIHPDYVITTQHWLGLLGPNGTQPQIANCSVYDFFVWLHYYSVRDTLLGPGRPYKAIDFSHQGPAFVTWHRYHLLWLERELQRLTGNESFALPYWNFATGKNECDVCTDELLGAARQDDPTLISRNSRFSTWEIVCDSLDDYNRRVTLCNGTYEGLLRRNKVGRNNEKLPTLKNVQDCLSLQKFDSPPFFQNSTFSFRNALEGFDKADGTLDSQVMNLHNLAHSFLNGTNALPHSAANDPVFVVLHSFTDAIFDEWLKRNNPSTDAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTAEQLGYNYAVDLSEEEAPVWSTTLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-SUMO-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

Recombinant Mouse L-dopachrome tautomerase (Dct)是一種重組的鼠源性L-多巴胺酚酮酶。Dct酶是一種重要的催化酶，在黑色素合成過(guò)程中起著關(guān)鍵的作用。它能夠?qū)-多巴銅離子催化轉(zhuǎn)化成多巴胺酮，并參與后續(xù)的黑色素合成反應(yīng)。由于Dct在黑色素合成中的重要作用，因此對(duì)其進(jìn)行研究有助于我們更好地理解黑色素生成的機(jī)制，以及相關(guān)的生理和病理過(guò)程。此外，Dct也被認(rèn)為可能與其他重要的生物過(guò)程相關(guān)，如細(xì)胞增殖和細(xì)胞分化等。
華美生物的重組的鼠源性Dct酶由大腸桿菌表達(dá)，表達(dá)區(qū)域?yàn)?4-472aa，具有良好的穩(wěn)定性和純度，可以被廣泛應(yīng)用于基礎(chǔ)科研及生物醫(yī)學(xué)研究中，如藥物開(kāi)發(fā)、疾病診斷和基因工程等領(lǐng)域。
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
基因功能參考文獻(xiàn)：
1. soyasaponin Ag inhibited TRP2 expression in a dosedependent manner. Therefore, the depigmenting effect of soyasaponin Ag may be due to the inhibition of tyrosinase expression or the enhancement of tyrosinase degradation. PMID: 28713957
2. the slaty mutant melanocytes seem to be more sensitive to ultraviolet rays and oxidative stress than black melanocytes. PMID: 26451690
3. A novel murine model of vitiligo is established by sequential prime/boost immunizations into the hind footpad and tail dermis with tyrosinase-related protein 2 (TRP2)-180 (SVYDFFVWL) peptide. PMID: 23711243
4. study indicates that tumor antigen (TRP2) expressed in MCMV induces a strong and long-lasting anti-melanoma effect through an antibody-dependent mechanism PMID: 23811402
5. Vaccine formulations with synthetic long peptides for treating B16F10 skin melanoma fails to elicit strong CD8 T cell responses to self-differentiation antigens TRP-2 and gp100. PMID: 23203930
6. level of gene targeting was not improved by the DSB induction, indicating a limited capacity of I-SceI to mediate homologous recombination at the Dct locus PMID: 22761925
7. Recombinant dopachrome tautomerase was expressed and purified, without its carboxy-terminal transmembrane region. Analysis of dopachrome tautomerase tryptic peptides by MALDI-TOF/TOF determined N-glycosylation as a primary post-translational modification. PMID: 20386969
8. Dopachrome tautomerase inactivation elevates the level of ROS, increases the numbers of sunburn cells and apoptotic cells, and decreases the amount of eumelanin in the epidermis upon exposure to chronic UVA radiation PMID: 20123016
9. the TRP-2 polypeptide folds in the endoplasmic reticulum (ER) in the presence of calnexin, until it reaches a dithiothreitol-resistant conformation enabling its ER exit to the Golgi PMID: 12719423
10. Results describe the generation of a knockout of the dopachrome tautomerase gene in mice with effects restricted to pigment production and coat color. PMID: 15060160
11. We found that ionophore monensin (Mon) and the quaternary amine chloroquine (CQ) discriminate between the traffic routes of TRP-2 and TRP-1 PMID: 15707965
12. The enzymatic activity of Dct may play a role in determining whether the eumelanin or pheomelanin pathway is preferred for pigment biosynthesis. PMID: 15960609
13. These results suggest that the slaty mutation affects both eumelanin and pheomelanin synthesis in developmental stage-specific and skin site-specific manners. PMID: 16584806
14. Slaty mutation blocks the melanosome maturation at stage III and affects the melanosome morphology (elliptical or spherical) in a developmental stage-specific manner. PMID: 17516460
15. Eumelanin and pheomelanin may accumulate with difficulty in slaty-mutated melanocytes and be easily released from them during skin development. L-Tyr may stimulate this release. PMID: 17551240
16. These results suggest that the slaty mutation inhibits the development of elliptical stage IV melanosomes and that L-Tyr restores the development of elliptical stage IV melanosomes PMID: 17867832
17. Cytotoxicity toward targets loaded with a K(b)-restricted tyrosinase-related protein 2-derived peptide correlated with depigmentation in a mouse model of autoimmune vitiligo. PMID: 18337834
18. mice lacking DCT displayed normal cardiac development but an increased susceptibility to atrial arrhythmias PMID: 19855129
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相關(guān)疾病：

The slaty mutation in Tyrp2 leads to a decrease of DT activity and a consequent change in the pigmentation of the mice to a dark gray/brown eumelanin. The slaty-2j mutation has a similar phenotype, the slaty-lt (light) mutation has a more severe effect and is semidominant; its phenotype may be a result of the failure of the enzyme to be correctly targeted to its normal location on the inner face of the melanosomal membrane.
亞細(xì)胞定位：

Melanosome membrane; Single-pass type I membrane protein. Melanosome.
蛋白家族：

Tyrosinase family
組織特異性：

Melanocytes and retinal pigmented epithelium (at protein level).
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:13190

STRING: 10090.ENSMUSP00000022725

UniGene: Mm.19987