Recombinant Mouse Kinesin-1 heavy chain (Kif5b), partial

中文名稱：

小鼠Kif5b重組蛋白
貨號：

CSB-YP737044MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Kif5b重組蛋白
貨號：

CSB-EP737044MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Kif5b重組蛋白
貨號：

CSB-EP737044MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Kif5b重組蛋白
貨號：

CSB-BP737044MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Kif5b重組蛋白
貨號：

CSB-MP737044MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Kif5b
Uniprot No.：

Q61768
別名：

Kif5b; Khcs; Kns1; Kinesin-1 heavy chain; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends. Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells.
基因功能參考文獻(xiàn)：
1. kinesin and microtubules can compensate for defects in myosin-Va and actin-based transport in mammals, but endogenous Kif5b does not have an important role in transport of melanocytes due to its inefficient recruitment to melanosomes PMID: 28490438
2. High KIF5B expression is associated with Lung Metastasis of Breast Cancer. PMID: 29033361
3. It was concluded that selective deletion of Kif5b in adipose tissue exacerbates HFD-induced obesity and its associated metabolic disorders, partly through a decrease in energy expenditure, dysregulation of adipokine secretion, and insulin signaling. PMID: 28242773
4. PI3K-dependent formation of a kinesin-1/Slp3/Rab27b complex is critical for the microtubule-dependent movement of secretory granules required for mast cell degranulation. PMID: 27810912
5. The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. PMID: 27264953
6. results indicate that NCAM is delivered to the cell surface through a kinesin-1-mediated transport mechanism in a PAK1-dependent manner. PMID: 26101351
7. KIF5B is essential for Ostm1 intracellular dispersion. PMID: 26598607
8. Kif5b expression during mouse kidney development PMID: 25885434
9. we showed that the KIF5B-RET fusion gene is oncogenic in lung alveolar epithelial cells in vivo. PMID: 25064355
10. nesprin-dependent recruitment of kinesin-1 to the nuclear envelope through the interaction of a conserved LEWD motif with kinesin light chain might be a general mechanism for cell-type-specific nuclear positioning during development. PMID: 25516977
11. KIF5B is critical in suppressing chromosomal instability at the early stages of female meiotic cell development and mitotic cell division. PMID: 23560038
12. These results suggest that while the KLC binding domain may directly targets Kif5b to the myonuclear membrane, the autoinhibitory peptide may play an indirect role in regulating the kinesin-1-mediated myonuclear positioning PMID: 23402760
13. Kif5b is responsible for the localization of alpha-sarcomeric actin, desmin and nestin in differentiating myoblast cells. PMID: 23293293
14. identification of the microtubule-associated protein ensconsin (Ens)/microtubule-associated protein 7 (MAP7) and kinesin heavy chain (Khc)/Kif5b as essential, evolutionarily conserved regulators of myonuclear positioning in Drosophila and cultured mammalian myotubes PMID: 22425998
15. Nesca directly binds KIF5B, kinesin light-chain and syntaxin-1 PMID: 22404429
16. Anterograde trafficking via kinesin 5B is essential for both receptor recycling from the phagosome and delivery of Rab11-containing membrane stores to effect broad and functional pseudopods during Fcgamma receptor-mediated phagocytosis. PMID: 21149599
17. Impairment of anterograde transport by knockdown of KIF5B or KLC1 delayed stress-granule dissolution. PMID: 19825938
18. data reveal that insulin signaling targets the engagement or initiates the movement of glutamate transporter 4-containing membranes on microtubules via KIF5B through a phosphatidylinositol 3-kinase-independent mechanism. PMID: 12743033
19. TNF produced by activated glial cells in inflammatory or degenerative neurological diseases acts on neurites by acting on the kinesin-tubulin complex and inhibits axonal mitochondria and synaptophysin transport via JNK. PMID: 17068110
20. binding of the kinesin-binding domain of RanBP2 to KIF5B and KIF5C determines mitochondria localization and function PMID: 17887960
21. Daxx and GLUT4 interact with JNK1 through D-sites in their NH(2)-(aa 1-501) and large endofacial loop, respectively. Daxx interacts with kinesin KIF5B through the 6xTPR domain of the kinesin light chain. PMID: 18932217
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Cytolytic granule membrane. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin subfamily
數(shù)據(jù)庫鏈接：

KEGG: mmu:16573

STRING: 10090.ENSMUSP00000025083

UniGene: Mm.223744