Recombinant Mouse Keratin, type I cytoskeletal 14 (Krt14)

中文名稱(chēng)：

小鼠Krt14重組蛋白
貨號(hào)：

CSB-YP713987MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

小鼠Krt14重組蛋白
貨號(hào)：

CSB-EP713987MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

小鼠Krt14重組蛋白
貨號(hào)：

CSB-EP713987MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

小鼠Krt14重組蛋白
貨號(hào)：

CSB-BP713987MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

小鼠Krt14重組蛋白
貨號(hào)：

CSB-MP713987MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Krt14
Uniprot No.：

Q61781
別名：

Krt14; Krt1-14; Keratin; type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-484
氨基酸序列

MATCSRQFTS SSSMKGSCGI GGGSSRMSSI LAGGSCRAPS TYGGMSVTSS RFSSGGACGI GGGYGGSFSS SSFGGGLGSG FGGRFDGFGG GFGGGLGGGF GGGLGGGLGG GIGDGLLVGS EKVTMQNLND RLATYLDKVR ALEEANTELE VKIRDWYQRQ RPTEIKDYSP YFKTIEDLKS KILAATVDNA NVLLQIDNAR LAADDFRTKF ETEQSLRMSV EADINGLRRV LDELTLARAD LEMQIESLKE ELAYLKKNHE EEMASMRGQV GGDVNVEMDA APGVDLSRIL NEMRDQYEKM AEKNRKDAEE WFFSKTEELN REVATNSELV QSGKSEISEL RRTMQNLEIE LQSQLSMKAS LENNLEETKG RYCMQLAQIQ EMIGSVEEQL AQLRCEMEQQ NQEYKILLDV KTRLEQEIAT YRRLLEGEDA HLSSSQFSSS SQFSSGSQSS RDVTSTNRQI RTKVMDVHDG KVVSTHEQVL RTKN
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
基因功能參考文獻(xiàn)：
1. Cumulatively, these data indicate that altered CK14 (high) and CK18 (low) expression is perhaps an early event in bladder cancer tumorigenesis in females at least and is characteristic of both urothelial superficial pre-neoplastic and neoplastic lesions. PMID: 29350066
2. KRT14 is expressed in a subpopulation of bladder basal cells with pivotal role in regeneration and tumorigenesis. PMID: 27320313
3. CDK1, Aurora-B, and Rho-kinase phosphorylate keratin 5/14. PMID: 29518391
4. RA regulates Krt5 and Krt14 expression independently of stem cell character in developing salivary epithelium. The stem cell gene Kit is regulated inversely from Krt5/Krt14 by RA signaling. PMID: 27884045
5. Analysis of K14 variants with single or multiple substitutions of cysteine residues points to a spatial and temporal hierarchy in how Cys-4/Cys-40 and Cys-367 regulate keratin assembly in vitro and filament dynamics in live keratinocytes PMID: 26216883
6. Disulfide bonding is a novel and important mechanism regulating the assembly, intracellular organization, and dynamics of K14-containing intermediate filaments in skin keratinocytes. PMID: 25869667
7. K14-VEGF transgenic mouse a valuable model to study novel immunotherapies for psoriasis PMID: 21210341
8. autoantibodies in Scurfy mice and patients with IPEX target keratin 14 PMID: 20147963
9. nipple connective tissue and its development: insights from the keratin 14-PTHrP mouse PMID: 12049768
10. The keratin-14 IL-4 transgenic (Tg) mouse model of atopic dermatitis (AD) is characterized by skin infiltration of T cells, early up-regulation of T(h)2 cytokines and late surge of T(h)1 cytokines. PMID: 16735375
11. These studies provide a potential mechanism by which deltaNp63 directly governs the expression of K14 in a keratinocyte-specific manner. PMID: 17159913
12. Study describes the expression profile of claudins and keratin markers in the accelerated epidermal permeability barrier formation of K14-CaSR transgenic mice during development as compared to the wild type from E12.5 to newborn stages. PMID: 17182288
13. DeltaNp63 alone can restore the expression of the basal keratins and reinitiate the failed epidermal differentiation program in the skin of p63 null animals. PMID: 19461998
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亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Intermediate filament family
組織特異性：

Expressed in the corneal epithelium (at protein level). Expressed in the epithelial basal layer in the tail epidermis. Expressed in the parabasal cell row, basal cell layer, and suprabasal epithelial layer of the tongue.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:16664

STRING: 10090.ENSMUSP00000007272

UniGene: Mm.439898