Recombinant Mouse Hyaluronidase PH-20 (Spam1), partial

中文名稱：

小鼠Spam1重組蛋白
貨號：

CSB-YP022471MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Spam1重組蛋白
貨號：

CSB-EP022471MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Spam1重組蛋白
貨號：

CSB-EP022471MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Spam1重組蛋白
貨號：

CSB-BP022471MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Spam1重組蛋白
貨號：

CSB-MP022471MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Spam1
Uniprot No.：

P48794
別名：

Spam1; Ph20; Spam; Hyaluronidase PH-20; Hyal-PH20; EC 3.2.1.35; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
表達區域：

-
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
基因功能參考文獻：
1. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
2. ACR or PRSS21 or both may function cooperatively with SPAM1 in sperm/cumulus penetration PMID: 22362218
3. The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase SPAM1 may not be required for fertilization, at least in the mouse. PMID: 19926939
4. Entrapment of spontaneous Spam1 mutations, owing to recombination suppression near the Robertsonion translocation junctions, is proposed as the major underlying defect of the sperm dysfunction. PMID: 11845284
5. sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg PMID: 12065596
6. Epididymal Spam1 is secreted predominantly as insoluble particles. Majority of epididymal Spam1 is released with its lipid anchor, a form in which it can bind to sperm. PMID: 12514083
7. Spam1 is a multifunctional protein and that it is less restricted in its expression than previously reported PMID: 12672666
8. Sperm adhesion molecule 1 (SPAM1) is distributed in the nonciliated epithelial cells. Efferent ducts have the most intense staining. Spam1 was also present in the accessory organs, the prostate, and seminal vesicles and its fluid. PMID: 15175239
9. Testicular compartmentalization of Spam1 RNA provides strong support for the lack of sharing hypothesis, and represents a possible mechanism of Spam1-associated transmission ratio distortion . PMID: 16092963
10. Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit hyaluronidase activity at neutral pH. PMID: 16925524
11. SPAM1 is implicated in fluid reabsorption and urine concentration PMID: 17446714
12. First documentation of vesicular interaction with the sperm plasma membrane provides a model for the possible transfer mechanism of SPAM1 and other glycosyl phosphatidylinositol (GPI)-linked proteins to the sperm surface in reproductive fluids. PMID: 18384048
13. SPAM1 may function in sperm entry into the cumulus and sperm penetration through the cumulus matrix. PMID: 19605784
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Glycosyl hydrolase 56 family
數據庫鏈接：

KEGG: mmu:20690

STRING: 10090.ENSMUSP00000031693

UniGene: Mm.4688